Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,235,088 G→C 8.6% P61P (CCG→CCC) ‡ PP_2834 → D‑galactarate dehydratase/altronate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,235,0880GC 8.6% 176.7 / 6.8 58P61P (CCG→CCC) ‡PP_2834D‑galactarate dehydratase/altronate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (29/24);  new base C (2/3);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

TCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAG  >  NC_002947/3234943‑3235224
                                                                                                                                                 |                                                                                                                                        
tCCGCCTCAACCCGATGGACAACGTCGTCATCGCCCGCCAGGCCCTGCGGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCATCAGGCCACCAGCTCGCCACCGCGCCAGTGGCGCCAGGCCAGCCGtgg                                                                                                                                       >  4:192240/1‑147 (MQ=255)
    ccTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGcc                                                                                                                                   <  1:173969/149‑1 (MQ=255)
        aaCCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTa                                                                                                                               >  6:69242/1‑149 (MQ=255)
        aaCCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAACGGCTGGCCTTGCGccacag                                                                                                                               <  7:58417/149‑5 (MQ=255)
          cccTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGCGGCGCAACGGCTGGCCTTGCGccac                                                                                                                                 >  1:177045/1‑143 (MQ=255)
          cccTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGCGGCGCAACGGCTGGCCTTGCGccac                                                                                                                                 <  2:177045/145‑3 (MQ=255)
             tttGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACggg                                                                                                                           >  5:289351/1‑148 (MQ=255)
               tGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCa                                                                                                                         <  2:8606/148‑1 (MQ=255)
                   cAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGatca                                                                                                                    >  4:16175/1‑149 (MQ=255)
                      cgtcgtCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCg                                                                                                                 >  7:249088/1‑149 (MQ=255)
                        tcgtcATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGa                                                                                                               <  5:76328/149‑1 (MQ=255)
                             aTCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGc                                                                                                          <  5:69242/149‑1 (MQ=255)
                                gcccgccAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGt                                                                                                        <  4:118665/148‑1 (MQ=255)
                                  ccgccAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGtt                                                                                                                                <  2:132532/122‑1 (MQ=255)
                                  ccgccAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGtt                                                                                                                                >  1:132532/1‑122 (MQ=255)
                                    gccAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAg                                                                                                   <  6:141558/149‑1 (MQ=255)
                                       aGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGc                                                                                                 >  1:261764/1‑148 (MQ=255)
                                                    aGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCg                                                                                   <  6:289351/149‑1 (MQ=255)
                                                             ccTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACcatgtg                                                                           >  2:336078/1‑148 (MQ=255)
                                                              cTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAACGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACcatgtgca                                                                         >  8:56932/1‑149 (MQ=255)
                                                                 gATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACcatgtgcatgt                                                                      <  3:16175/149‑1 (MQ=255)
                                                                  aTGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACcatgtgcatgtg                                                                     >  8:224335/1‑149 (MQ=255)
                                                                   tGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCatgtgcatgtgc                                                                    <  7:131678/149‑1 (MQ=255)
                                                                      cGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGcac                                                                  >  6:178975/1‑148 (MQ=255)
                                                                            gATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGc                                                           <  1:336078/149‑1 (MQ=255)
                                                                            gATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGc                                                           <  5:193855/149‑1 (MQ=255)
                                                                                    tGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGc                                                   >  1:202144/1‑149 (MQ=255)
                                                                                    tGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCACCGTTACGGGCAGatcatc                                                                                                                  <  3:149630/86‑1 (MQ=255)
                                                                                    tGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCACCGTTACGGGCAGatcatc                                                                                                                  >  4:149630/1‑86 (MQ=255)
                                                                                          aGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGAtttt                                             <  6:129480/149‑1 (MQ=255)
                                                                                             ccATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCatgtgcatgtgc                                                                    >  3:308968/1‑123 (MQ=255)
                                                                                             ccATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCatgtgcatgtgc                                                                    <  4:308968/123‑1 (MQ=255)
                                                                                               aTCCCTTCAGGCCACGAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCcg                                        >  4:102627/1‑149 (MQ=255)
                                                                                               aTCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCcg                                        >  5:335331/1‑149 (MQ=255)
                                                                                                   cTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGAc                                    >  2:169838/1‑149 (MQ=255)
                                                                                                    ttCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATCTGCACGTTGTGCACATgcacat                                                 <  7:27971/135‑6 (MQ=255)
                                                                                                    ttCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATCTGCACGTTGTGCACATgcacat                                                 >  8:27971/1‑130 (MQ=255)
                                                                                                       aGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTAt                                 <  7:56932/148‑1 (MQ=255)
                                                                                                          ccACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGt                             >  2:219519/1‑149 (MQ=255)
                                                                                                          ccACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGt                             <  2:261764/149‑1 (MQ=255)
                                                                                                            acaAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGa                                                 <  6:133917/127‑1 (MQ=255)
                                                                                                            acaAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGa                                                 >  5:133917/1‑127 (MQ=255)
                                                                                                            acaAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGttt                           <  3:192240/149‑1 (MQ=255)
                                                                                                              aaGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCcgcg                                      <  4:158759/136‑1 (MQ=255)
                                                                                                              aaGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCcgcg                                      >  3:158759/1‑136 (MQ=255)
                                                                                                              aaGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTgg                         >  4:256826/1‑149 (MQ=255)
                                                                                                                 cTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGgtgt                      >  6:258806/1‑149 (MQ=255)
                                                                                                                    gCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTc                     <  5:178975/147‑1 (MQ=255)
                                                                                                                       aCCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATAc                >  2:187527/1‑149 (MQ=255)
                                                                                                                         cGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATAccc              >  5:49909/1‑149 (MQ=255)
                                                                                                                               tgtgGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGcc        >  6:115684/1‑149 (MQ=255)
                                                                                                                                gtgGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCt       <  6:335331/149‑1 (MQ=255)
                                                                                                                                acggCGCAACGGCTGGCCTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGc         >  4:44619/3‑147 (MQ=255)
                                                                                                                                acggCGCAACGGCTGGCCTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGc         <  3:44619/145‑1 (MQ=255)
                                                                                                                                 tgGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTg      >  2:207690/1‑149 (MQ=255)
                                                                                                                                     gcAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAg  >  7:175720/1‑149 (MQ=255)
                                                                                                                                            cAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGAtt                                               >  4:244953/1‑97 (MQ=255)
                                                                                                                                            cAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGAtt                                               <  3:244953/97‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                        
TCCGCCTCAACCCTTTGGACAACGTCGTCATCGCCCGCCAGGCCCTGCTGCCAGGCGTGCGCCTGGATGCCGAGGCGATTACCGTGCGCCAGCCCATCCCTTCAGGCCACAAGCTCGCCACCGAGCCTGTGGCGCAAGGCCAGCCGTTGCGCCGTTACGGGCAGATCATCGGATTTGCGTCCCAGGCGATCGACGCTGGCGACCATGTGCATGTGCACAACGTGCAGATGGGCGATTTTGCCCGCGACTATGCGTTTGGTGTCGATACCCGCAGCCTGCCAG  >  NC_002947/3234943‑3235224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑