Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,266,095	A→T	16.6%	intergenic (+12/+47)	PP_2863 → / ← PP_2864	membrane protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,266,095	0	A	T	16.6%	110.7 / 12.1	48	intergenic (+12/+47)	PP_2863/PP_2864	membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (18/22);  new base T (4/4);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

TTGGCGCAGCAGCTGTTTCGGCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGG  >  NC_002947/3265949‑3266240                                                                                                                                                   |                                                                                                                                                  ttGGCGCAGCAGCTGTTTCGGCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTAcc                                                                                                                                                 <  5:254962/149‑1 (MQ=255)              tGTTTCGGCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCgg                                                                                                                                    >  4:265455/1‑149 (MQ=255)                tttCGGCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCgg                                                                                                                                    <  4:44688/147‑1 (MQ=255)                tttCGGCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGt                                                                                                                                  <  5:244621/149‑1 (MQ=255)                tttCGGCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGt                                                                                                                                  <  7:198310/149‑1 (MQ=255)                               cGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGACCCTTTCGCGGCTTTACCCGCGaagagg                                                                                                                   >  1:92226/1‑145 (MQ=255)                                gCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGGGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGc                                                                                                                  <  4:14885/149‑1 (MQ=255)                                  aaGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGACCCTTTCGCGGCTTTACCCGCGAAGAGGCCg                                                                                                                <  2:92226/149‑1 (MQ=255)                                     gTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGaa                                                                                                             <  6:301866/149‑1 (MQ=255)                                         cGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAg                                                                                                          <  2:253133/148‑1 (MQ=255)                                           ttCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCTTTCGCGGCTTTACCCGCGaagagg                                                                                                                   <  5:335255/137‑5 (MQ=38)                                           ttCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCTTTCGCGGCTTTACCCGCGaagagg                                                                                                                   >  6:335255/1‑133 (MQ=38)                                           ttCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTc                                                                                                       >  1:68146/1‑149 (MQ=255)                                             ccAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGa                                                                                                     >  6:253242/1‑149 (MQ=255)                                              cAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCGGTACaac                                                                                                         >  2:317629/1‑142 (MQ=25)                                              cAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCGGTACaac                                                                                                         <  5:287524/144‑3 (MQ=25)                                              cAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCGGTACaac                                                                                                         <  1:317629/144‑3 (MQ=25)                                              cAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCGGTACaac                                                                                                         >  6:287524/1‑142 (MQ=25)                                                     tgtgCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGa                                                                                             <  5:253242/149‑1 (MQ=255)                                                         cTGTGGGATTGTGCTTCTTCACCAGGTGTTCTGGGCAGAGTTGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGTTGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCaa                                                                                         <  8:238745/149‑1 (MQ=255)                                                          tGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAAc                                                                                        >  2:308595/1‑149 (MQ=255)                                                           gggggATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACa                                                                                                           <  2:148258/129‑1 (MQ=255)                                                           gggggATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCATCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACa                                                                                                           >  1:148258/1‑129 (MQ=255)                                                               gATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCAc                                                                                   <  3:265455/149‑1 (MQ=255)                                                                            cACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTg                                                                      <  2:68146/149‑1 (MQ=255)                                                                                       cTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACg                                                           >  2:337926/1‑149 (MQ=255)                                                                                             aGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTAc                                                     <  5:46025/149‑1 (MQ=255)                                                                                              gACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTAcc                                                    <  5:104926/149‑1 (MQ=255)                                                                                               aCTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCa                                                   <  1:308595/149‑1 (MQ=255)                                                                                                 tGGCTGTTTCTTGACGGGCATTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTg                                                                      >  1:103953/1‑128 (MQ=255)                                                                                                 tGGCTGTTTCTTGACGGGCATTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTg                                                                      <  2:103953/128‑1 (MQ=255)                                                                                                   gCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTg                                               >  4:272401/1‑149 (MQ=255)                                                                                                     tGTTTCTTGACGGGCGTTATCTACCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTg                                                                      >  2:78250/1‑124 (MQ=255)                                                                                                     tGTTTCTTGACGGGCGTTATCTACCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTg                                                                      <  1:78250/124‑1 (MQ=255)                                                                                                             gACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGc                                     >  6:104751/1‑149 (MQ=255)                                                                                                             gACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGc                                     >  7:240450/1‑149 (MQ=255)                                                                                                                            ccccTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTcaca                       >  4:135577/1‑148 (MQ=255)                                                                                                                            ccccTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTc                          <  8:240450/145‑1 (MQ=255)                                                                                                                              ccTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAg                    >  4:128682/1‑149 (MQ=255)                                                                                                                                    ttGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTAc                                                     <  5:346148/110‑1 (MQ=255)                                                                                                                                    ttGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTAc                                                     >  6:346148/1‑110 (MQ=255)                                                                                                                                         gtgCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCAcc                                                                                  <  7:153935/76‑1 (MQ=255)                                                                                                                                         gtgCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCAcc                                                                                  >  8:153935/1‑76 (MQ=255)                                                                                                                                          tgCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGAGCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGccc        >  5:241585/1‑149 (MQ=255)                                                                                                                                              gTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATg    >  3:290563/1‑149 (MQ=255)                                                                                                                                               ctGTTCCGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCGGTACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAacac                             >  4:96533/2‑123 (MQ=25)                                                                                                                                               ctGTTCCGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCGGTACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAacac                             <  3:96533/122‑1 (MQ=25)                                                                                                                                                 gTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATggg  <  1:337926/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  TTGGCGCAGCAGCTGTTTCGGCACAAGACCCGCAAGGTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGG  >  NC_002947/3265949‑3266240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑