Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,266,132	A→T	14.0%	intergenic (+49/+10)	PP_2863 → / ← PP_2864	membrane protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,266,132	0	A	T	14.0%	105.7 / 9.1	43	intergenic (+49/+10)	PP_2863/PP_2864	membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (19/18);  new base T (3/3);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

GTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGCTGACCCACCGC  >  NC_002947/3265985‑3266268                                                                                                                                                    |                                                                                                                                         gTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGaa                                                                                                                                         <  6:301866/149‑1 (MQ=255)     cGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAg                                                                                                                                      <  2:253133/148‑1 (MQ=255)       ttCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTc                                                                                                                                   >  1:68146/1‑149 (MQ=255)         ccAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGa                                                                                                                                 >  6:253242/1‑149 (MQ=255)          cAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCGGTACaac                                                                                                                                     <  5:287524/144‑3 (MQ=25)          cAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCGGTACaac                                                                                                                                     <  1:317629/144‑3 (MQ=25)          cAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCGGTACaac                                                                                                                                     >  2:317629/1‑142 (MQ=25)          cAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTTCCGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCGGTACaac                                                                                                                                     >  6:287524/1‑142 (MQ=25)                 tgtgCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGa                                                                                                                         <  5:253242/149‑1 (MQ=255)                     cTGTGGGATTGTGCTTCTTCACCAGGTGTTCTGGGCAGAGTTGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGTTGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCaa                                                                                                                     <  8:238745/149‑1 (MQ=255)                      tGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAAc                                                                                                                    >  2:308595/1‑149 (MQ=255)                       gggggATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACa                                                                                                                                       <  2:148258/129‑1 (MQ=255)                       gggggATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCATCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACa                                                                                                                                       >  1:148258/1‑129 (MQ=255)                           gATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCAc                                                                                                               <  3:265455/149‑1 (MQ=255)                                        cACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTg                                                                                                  <  2:68146/149‑1 (MQ=255)                                                   cTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACg                                                                                       >  2:337926/1‑149 (MQ=255)                                                         aGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTAc                                                                                 <  5:46025/149‑1 (MQ=255)                                                          gACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTAcc                                                                                <  5:104926/149‑1 (MQ=255)                                                           aCTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCa                                                                               <  1:308595/149‑1 (MQ=255)                                                             tGGCTGTTTCTTGACGGGCATTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTg                                                                                                  <  2:103953/128‑1 (MQ=255)                                                             tGGCTGTTTCTTGACGGGCATTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTg                                                                                                  >  1:103953/1‑128 (MQ=255)                                                               gCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTg                                                                           >  4:272401/1‑149 (MQ=255)                                                                 tGTTTCTTGACGGGCGTTATCTACCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTg                                                                                                  >  2:78250/1‑124 (MQ=255)                                                                 tGTTTCTTGACGGGCGTTATCTACCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTg                                                                                                  <  1:78250/124‑1 (MQ=255)                                                                         gACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGc                                                                 >  7:240450/1‑149 (MQ=255)                                                                         gACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGc                                                                 >  6:104751/1‑149 (MQ=255)                                                                                        ccccTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTcaca                                                   >  4:135577/1‑148 (MQ=255)                                                                                        ccccTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTc                                                      <  8:240450/145‑1 (MQ=255)                                                                                          ccTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAg                                                >  4:128682/1‑149 (MQ=255)                                                                                                ttGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTAc                                                                                 <  5:346148/110‑1 (MQ=255)                                                                                                ttGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTAc                                                                                 >  6:346148/1‑110 (MQ=255)                                                                                                     gtgCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCAcc                                                                                                              >  8:153935/1‑76 (MQ=255)                                                                                                     gtgCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCAcc                                                                                                              <  7:153935/76‑1 (MQ=255)                                                                                                      tgCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGAGCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGccc                                    >  5:241585/1‑149 (MQ=255)                                                                                                          gTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATg                                >  3:290563/1‑149 (MQ=255)                                                                                                           ctGTTCCGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCGGTACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAacac                                                         >  4:96533/2‑123 (MQ=25)                                                                                                           ctGTTCCGGCCCTTTCGCGGCTTTACCCGCGAAGAGGCCGGTACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAacac                                                         <  3:96533/122‑1 (MQ=25)                                                                                                             gTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATggg                              <  1:337926/148‑1 (MQ=255)                                                                                                                                  aaGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGCTGAcc        <  5:104751/149‑1 (MQ=255)                                                                                                                                     ccGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGCTGACCCAc     >  4:772/1‑149 (MQ=255)                                                                                                                                      cgcgAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGCTGACCCAcc    >  3:19461/1‑149 (MQ=255)                                                                                                                                        cgAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGCTGACCCAccgc  <  3:128682/149‑1 (MQ=255)                                                                                                                                              ggCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTCGGGCAACCCACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGCTGAc         <  7:320514/136‑1 (MQ=255)                                                                                                                                              ggCCGGAACAGGTCGATCACATGACCAACCCCAACTGCAACCGCTTGGGCAACCGCCGCACTACCAGCTCGTGCGACCGCTGCAACACCTCACACAGATCATCACGACCCATCGGGTAGGGCTGCGCCATGCTGAc         >  8:320514/1‑136 (MQ=255)                                                                                                                                                    |                                                                                                                                         GTTTCGTTCCAGGTGGTGTGCTGGGGGATTGTGCTGCTGCACCAGGTGTTCTGGGCAGACTGGCTGTTTCTTGACGGGCGTTATCTGCCCCTTGGTTGATGTGCAGTTGTACCGGCCTCTTCGCGGGTAAAGCCGCGAAAGGGCCGGAACAGGTCGATCACATGACCAACCCCACCTGCAACCGCTTGGGCAACCGACGCACTACCAGCTGGTGCGACCGCTGCAACACCTCACACAGTTCATCACGCCCCATGGGGTAGGGCGGCGCCATGCTGACCCACCGC  >  NC_002947/3265985‑3266268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑