Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,412,901 C→T 8.3% intergenic (‑77/‑90) PP_3025 ← / → PP_3026 LysE family transporter/recombinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,412,9010CT 8.3% 136.8 / 2.6 48intergenic (‑77/‑90)PP_3025/PP_3026LysE family transporter/recombinase
Reads supporting (aligned to +/- strand):  ref base C (21/23);  new base T (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

TGCCATGTTGAGTGCAAAGCAGGCTGGTATGAATATGAGAAGTGTCGAAATGTTCATGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAGGGAGTGGTCTACAGCTATCTTCGGTTCTCCGA  >  NC_002947/3412768‑3413037
                                                                                                                                     |                                                                                                                                        
tGCCATGTTGAGTGCAAAGCAGGCTGGTATGAATATGAGAAGTGTCGAAATGTTCATGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGgcgcg                                                                                                                           <  4:185905/149‑1 (MQ=255)
 gCCATGTTGAGTGCAAAGCAGGCTGGTATGAATATGAGAAGTGTCGAAATGTTCATGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGgcgcgc                                                                                                                          >  3:128423/1‑149 (MQ=255)
       ttGAGTGCAAAGCAGGCTGGTATGAATATGAGAAGTGTCGAAATGTTCATGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCAtgt                                                                                                                    >  2:91487/1‑149 (MQ=255)
               aaaGCAGGCTGGTATGAATATGAGAAGTGTCGAAATGTTCATGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTg                                                                                                            <  1:159498/149‑1 (MQ=255)
                   cAGGCTGGTATGAATCTGAGAAGTGTCGAAATGGTCATGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCgt                                                                                                        <  7:198357/149‑1 (MQ=255)
                      gCTGGTATGAATATGAGAAGTGTCGAAATGTTCATGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTc                                                                                                     >  7:291635/1‑149 (MQ=255)
                      gCTGGTATGAATATGAGAAGTGTCGAAATGTTCATGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTc                                                                                                     >  6:132816/1‑149 (MQ=255)
                            aTGAATATGAGAAGTGTCGAAATGTTCATGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCTGTTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTg                                                                                                >  1:312755/1‑148 (MQ=255)
                            aTGAATATGAGAAGTGTCGAAATGTTCATGGGGTGCACATTATTTTTGTGAAGGTTGATACAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGTTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTg                                                                                                >  3:69074/1‑148 (MQ=255)
                               aaTATGAGAAGTGTCGAAATGTTCATGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAgg                                                                                            >  6:337992/1‑149 (MQ=255)
                                     agaAGTGTCGAAATGTTCATGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAat                                                                                       <  3:263564/148‑1 (MQ=255)
                                         gtgtCGAAATGTTCATGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAAtatata                                                                                  <  6:177468/149‑1 (MQ=255)
                                            tCGATATGTTCATGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTc                                                                                                                        <  6:85797/108‑1 (MQ=255)
                                            tCGATATGTTCATGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTc                                                                                                                        >  5:85797/1‑108 (MQ=255)
                                            tCGAAATGTTCATGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATActc                                                                               >  8:169433/1‑149 (MQ=255)
                                                       aTGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCg                                                                    >  1:63304/1‑149 (MQ=255)
                                                       aTGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCg                                                                    <  4:128423/149‑1 (MQ=255)
                                                          gggTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGt                                                                  <  7:169433/148‑1 (MQ=255)
                                                           ggTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTg                                                                <  2:63304/149‑1 (MQ=255)
                                                               cacaTTATTTTTGTGAAGTTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGt                                                            <  4:124947/149‑1 (MQ=255)
                                                                       ttttGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAg                                                    >  5:326629/1‑149 (MQ=255)
                                                                         ttGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGTTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCGCGGTTGGAGTGAAATCAgg                                                   <  4:69074/148‑1 (MQ=255)
                                                                         ttGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGTTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAgg                                                   <  2:312755/148‑1 (MQ=255)
                                                                         ttGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAgg                                                   <  5:337992/148‑1 (MQ=255)
                                                                           gtgAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATActct                                                                              <  7:1185/119‑1 (MQ=255)
                                                                           gtgAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATActct                                                                              >  8:1185/1‑119 (MQ=255)
                                                                           gtgAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGt                                                 <  8:291635/148‑1 (MQ=255)
                                                                                  ttGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGc                                         <  1:91487/149‑1 (MQ=255)
                                                                                      tCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGtct                                     >  8:315187/1‑149 (MQ=255)
                                                                                      tCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGtct                                     >  1:147749/1‑149 (MQ=255)
                                                                                       ccAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAgg                                                   >  7:105610/1‑134 (MQ=255)
                                                                                       ccAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAgg                                                   <  8:105610/134‑1 (MQ=255)
                                                                                         aGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAg                                  >  1:9480/1‑149 (MQ=255)
                                                                                             atcatTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAGGGAg                              >  4:54003/1‑149 (MQ=255)
                                                                                              tcatTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAGGGAGt                             >  6:300499/1‑149 (MQ=255)
                                                                                                   gatgatGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAGGGAGTg                            >  8:24383/1‑145 (MQ=255)
                                                                                                   gatgatGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAGGGAGTg                            <  7:24383/145‑1 (MQ=255)
                                                                                                   gatgatGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAGGGAGTGGTc                         <  6:326629/148‑1 (MQ=255)
                                                                                                            gTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATTTAATCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAGGGAGTGGTCTTCAGCTATc               >  7:225942/1‑149 (MQ=255)
                                                                                                               tGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAGGGAGTGGTCTACAGCTATCtt             <  2:9480/148‑1 (MQ=255)
                                                                                                                 gATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAGGGAGTGGTCTACAGCTATCTTCgg          <  5:300499/149‑1 (MQ=255)
                                                                                                                 gATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAGGGAGTGGTCTACAGCTATCTTCgg          <  3:54003/149‑1 (MQ=255)
                                                                                                                      tCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAGGGAGTGGTCTACAGCTATCTTCGGTtct      <  2:147749/148‑1 (MQ=255)
                                                                                                                       cAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAGGGAGTGGTCTACAGCTATCTTCGGTtctc     <  7:315187/148‑1 (MQ=255)
                                                                                                                       cAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGCCGTGTCCGGTGAAGGCTAATATAAACTCTGGCTTTCCCGGTTGGAGTGAAAACACGGTATGCAAGCGTCTCAGGGAGTCGTCTAAAGCTATCTTCTGttat      >  8:72467/1‑145 (MQ=255)
                                                                                                                         aTCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAGGGAGTGGTCTACAGCTATCTTCGGTTCTCCGa  >  2:269930/1‑149 (MQ=255)
                                                                                                                                   aGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAGGGAGTGGTc                         >  1:24840/1‑116 (MQ=255)
                                                                                                                                   aGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAGGGAGTGGTc                         <  2:24840/116‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
TGCCATGTTGAGTGCAAAGCAGGCTGGTATGAATATGAGAAGTGTCGAAATGTTCATGGGGTGCACATTATTTTTGTGAAGGTTGATCCAGTCATCATTGATGATGTCGTTTGGATTTTCAATCCCCAGGCAGCTAGGCTGTTGGCGCGCTCATGTGTTTTTTGGCGTGTCCGGTGAAGGGTAATATATACTCTGGCTTTCCCGGTTGGAGTGAAATCAGGGTATGCAAGCGTCTCAGGGAGTGGTCTACAGCTATCTTCGGTTCTCCGA  >  NC_002947/3412768‑3413037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑