Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,444,153 C→T 8.2% A971V (GCC→GTC)  PP_3062 → tail length determination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,444,1530CT 8.2% 172.8 / 4.2 61A971V (GCC→GTC) PP_3062tail length determination protein
Reads supporting (aligned to +/- strand):  ref base C (28/28);  new base T (3/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.10e-01

TGGTACGGCCGCAGGCCGAGCCCGAGCCGCCGACACCCAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGGCGCCCAAGCTGCCGGAGGTGCCGCCACCT  >  NC_002947/3444006‑3444301
                                                                                                                                                    |                                                                                                                                                    
tGGTACGGCCGCAGGCCGAGCCCGAGCCGCCGCCACCCAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGG‑CGAAGGATCCGTTCCTGTTGCCCGc                                                                                                                                                      >  5:23778/1‑148 (MQ=255)
  gTACGGCCGCAGGCCGAGCCCGAGCCGCCGACACCCAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGTCCGAAAGATCCGTTCCTGTTGCCCGcc                                                                                                                                                     <  1:3502/148‑1 (MQ=255)
   tACGGACGCAGGCCGAGCCAGACCCGCCGACACCCAAACTGGGGACTTCGGTGCGCGAAGCAGCAACGGCCACCGTACCGGCAAAGCCAGAAGTGACGTATGACACCACAGACACCCCGT‑CCAAAGATCCATTCCTCTAGCCCGCCAt                                                                                                                                                   >  8:140229/1‑148 (MQ=255)
         cGCAGGCCGAGCCCGAGCCGCCGACACCCAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGc                                                                                                                                             >  8:154320/1‑148 (MQ=255)
                    cccgagccGCCGACACCCAAACTGGGGGCTGCTGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCTACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCg                                                                                                                                 <  6:244473/149‑1 (MQ=255)
                     ccgagccGCCGACACCCAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCgg                                                                                                                                <  2:247939/149‑1 (MQ=255)
                      cgagccGCCGACACCCAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTg                                                                                                                                   >  6:33194/1‑145 (MQ=255)
                      cgagccGCCGACACCCAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTg                                                                                                                                   <  5:33194/145‑1 (MQ=255)
                             ccgACACCCAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAgg                                                                                                                        <  7:140229/149‑1 (MQ=255)
                                 cacCCAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGcc                                                                                                                    <  6:135898/149‑1 (MQ=255)
                                 cacCCAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGcc                                                                                                                    <  2:178195/149‑1 (MQ=255)
                                  acCCAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGcc                                                                                                                    >  1:45186/1‑148 (MQ=255)
                                  acCCAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGcc                                                                                                                    >  8:47076/1‑148 (MQ=255)
                                    ccAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCa                                                                                                                          <  2:8534/140‑1 (MQ=255)
                                    ccAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCa                                                                                                                          >  1:8534/1‑140 (MQ=255)
                                    ccAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGAc                                                                                                                 >  2:243976/1‑149 (MQ=255)
                                    ccAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGAc                                                                                                                 >  8:63599/1‑149 (MQ=255)
                                      aaaCTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGcc                                                                                                                    <  2:241921/144‑1 (MQ=255)
                                      aaaCTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGcc                                                                                                                    >  1:241921/1‑144 (MQ=255)
                                             gggCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCgg                                                                                                        <  7:154320/149‑1 (MQ=255)
                                              ggCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACGCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGc                                                                                                       <  3:308581/149‑1 (MQ=255)
                                               gCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGc                                                                                                       >  1:203103/1‑148 (MQ=255)
                                                     gtgcggGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAACGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGcc                                                                                                <  6:23778/149‑1 (MQ=255)
                                                      tgcggGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCgg                                                                                                                                <  6:50664/116‑1 (MQ=255)
                                                      tgcggGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCgg                                                                                                                                >  5:50664/1‑116 (MQ=255)
                                                          ggAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAAc                                                                                           <  7:47076/149‑1 (MQ=255)
                                                                   aCCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCTGAGCCAc                                                                                  >  2:344770/1‑149 (MQ=255)
                                                                        ccccGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGcc                                                                             >  1:291551/1‑149 (MQ=255)
                                                                          ccGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCg                                                                            >  6:8849/1‑148 (MQ=255)
                                                                                ccGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACATGGTGCGGTTCCCAGGCGCCGACATTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTgg                                                                      <  5:239258/148‑1 (MQ=255)
                                                                                ccGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCTACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTgg                                                                      <  1:243976/148‑1 (MQ=255)
                                                                                  ggCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTg                                                                   <  7:63599/149‑1 (MQ=255)
                                                                                   gCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCcac                                                                                                    <  1:79480/115‑1 (MQ=255)
                                                                                   gCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCcac                                                                                                    >  2:79480/1‑115 (MQ=255)
                                                                                   gCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCTGAGCCACCGGCCGTACTGGGTGa                                                                  <  1:344770/149‑1 (MQ=255)
                                                                                   gCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGa                                                                  >  7:44690/1‑149 (MQ=255)
                                                                                   gCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGa                                                                  >  7:58028/1‑149 (MQ=255)
                                                                                          ccGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCa                                                                                             <  5:18310/115‑1 (MQ=255)
                                                                                          ccGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCa                                                                                             >  6:18310/1‑115 (MQ=255)
                                                                                                    gTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGa                                                                                                                  >  8:272420/1‑84 (MQ=255)
                                                                                                    gTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGa                                                                                                                  <  7:272420/84‑1 (MQ=255)
                                                                                                        gACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGcc                                               >  7:263271/1‑147 (MQ=255)
                                                                                                              cgcgACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCcgc                                       <  5:8849/149‑1 (MQ=255)
                                                                                                                cgACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCgcgc                                     <  2:291551/149‑1 (MQ=255)
                                                                                                                       gCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGgc                              <  8:263271/149‑1 (MQ=255)
                                                                                                                        cGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGgcg                             <  3:295880/149‑1 (MQ=255)
                                                                                                                         gT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATTGCCAAGGCCGCGCCATCGgcgc                            <  4:89646/149‑1 (MQ=255)
                                                                                                                             gAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGGCGccc                          >  1:53487/1‑148 (MQ=255)
                                                                                                                             gAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGGCGccc                          >  5:263719/1‑148 (MQ=255)
                                                                                                                                     cGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGGCGCCCAAGCTGCCg                 >  8:7194/1‑149 (MQ=255)
                                                                                                                                      gTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGGCGCCCAAGCTGCCgg                <  8:58028/149‑1 (MQ=255)
                                                                                                                                      gTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGGCGCCCAAGCTGCCg                 >  2:129000/1‑148 (MQ=255)
                                                                                                                                          ttGTTGGCCGTCATGGCGGGCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGgc                              >  7:197640/2‑131 (MQ=255)
                                                                                                                                          ttGTTGGCCGTCATGGCGGGCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGgc                              <  8:197640/130‑1 (MQ=255)
                                                                                                                                          ttGTTGGCCGTCATGGCGGGCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGGCGCCCAAg                       <  8:195536/137‑1 (MQ=255)
                                                                                                                                          ttGTTGGCCGTCATGGCGGGCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGGCGCCCAAg                       >  7:195536/2‑138 (MQ=255)
                                                                                                                                          cTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCAGCGCCATCGGCGCCCACGCTGCCGGAGGt            >  7:129763/1‑149 (MQ=255)
                                                                                                                                             ttGGCCGTCATGGCGGGCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCATAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGGCGCCCAAGCTGCCGGAGGTgcc         >  2:315475/1‑149 (MQ=255)
                                                                                                                                             ttGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGGCGCCCAAGCTGCCGGAGGTgc          <  8:44690/148‑1 (MQ=255)
                                                                                                                                                cccGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGGCGCCCAAGCTGCCGGAGGTgccgcc      >  7:203497/1‑149 (MQ=255)
                                                                                                                                                cccGCCATGACGGCAAACAAGGTGCGGTTCCCAGGCGCCGCCCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGGCGCCCAAGCTGCCGGAGGTgccgcc      >  8:192888/1‑149 (MQ=255)
                                                                                                                                                   gCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGgc                              >  3:90863/1‑122 (MQ=255)
                                                                                                                                                   gCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGgc                              <  4:90863/122‑1 (MQ=255)
                                                                                                                                                    ccATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCACCCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGCCCGCGCCATCGGCGCCCAAGCTGCCGGAGGTGCCGCCACCt  >  1:135296/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGTACGGCCGCAGGCCGAGCCCGAGCCGCCGACACCCAAACTGGGGGCTGCGGTGCGGGAAGCAGCACCGGCCCCGGTACCGGCCAAGCCCGTAGTGTCGTATGACCCGCGCGACCCGGCGT‑CGAAAGATCCGTTCCTGTTGCCCGCCATGACGGCCAACAAGGTGCGGTTCCCAGGCGCCGACCTTGTGCGGCCACAGCCTCAACCCGAGCCACCGGCCGTACTGGGTGATGTGGTGCGCGAGATGGCCAAGGCCGCGCCATCGGCGCCCAAGCTGCCGGAGGTGCCGCCACCT  >  NC_002947/3444006‑3444301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑