Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,457,228 T→C 6.2% I21V (ATC→GTC)  hbdH ← 3‑hydroxybutyrate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,457,2280TC 6.2% 188.6 / 2.3 64I21V (ATC→GTC) hbdH3‑hydroxybutyrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (29/31);  new base C (2/2);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

AAAGCCTCGATCTGGACCACATCCGACAGGTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTGGCGATGACCACGAACACCGCCAGGGTCTTGATCAGGGTAATACCAAAAAT  >  NC_002947/3457080‑3457376
                                                                                                                                                      |                                                                                                                                                    
aaaGCCTCGATCTGGACCACATCCGACAGGTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGAt                                                                                                                                                      >  4:272104/1‑149 (MQ=255)
aaaGCCTCGATCTGGACCACATCCGACAGGTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGAt                                                                                                                                                      >  8:235856/1‑149 (MQ=255)
     cTCGATCTGGACCACATCCGACAGGTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCa                                                                                                                                                 <  7:235856/149‑1 (MQ=255)
         aTCTGGACCACATCCGACAGGTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGc                                                                                                                                              >  2:20181/1‑148 (MQ=255)
              gACCACATCCGACAGGTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATGCCTGGGCATCGCCCAggta                                                                                                                                             <  1:121875/144‑3 (MQ=255)
              gACCACATCCGACAGGTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCa                                                                                                                                            <  6:202915/145‑1 (MQ=255)
              gACCACATCCGACAGGTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGc                                                                                                                                        <  3:121802/149‑1 (MQ=255)
              gACCACATCCGACAGGTCGGCCGGGTGGTGCACAACCTTCACCCAGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCATCGCCCAggta                                                                                                                                             <  6:332091/144‑3 (MQ=255)
                ccACATCCGACAGGTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCg                                                                                                                                      >  8:310520/1‑149 (MQ=255)
                 cacaTCCGACAGGTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGc                                                                                                                                     >  6:316830/1‑149 (MQ=255)
                   caTCCGACAGGTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCtg                                                                                                                                   >  4:38295/1‑149 (MQ=255)
                     tCCGACAGGTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCtggt                                                                                                                                 >  5:122267/1‑149 (MQ=255)
                     tCCGACAGGTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCtggt                                                                                                                                 <  8:14602/149‑1 (MQ=255)
                       cGACAGGGCGGCCGGGGTGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCtggtgg                                                                                                                               <  4:194807/149‑1 (MQ=255)
                           aGGTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAAcc                                                                                                                           >  2:286025/1‑149 (MQ=255)
                            tgtcgtcCGGGTGGTGAACAACCTTCACCCCTTGCCGGGCAATTTCCGCCATGGCGGGTCCCGGGTCACCAAAGACGTTGAGCACGATGT‑‑TGGCGACAGCCAGGTCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGTTGGAACCg                                                                                                                          <  6:203857/148‑1 (MQ=255)
                             gTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAg                                                                                                                                                >  5:182236/1‑126 (MQ=255)
                             gTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAg                                                                                                                                                <  6:182236/126‑1 (MQ=255)
                              tCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGt                                                                                                                        <  3:216231/149‑1 (MQ=255)
                               cgggcgggGGGGGCACAACCCTCCCCCCGTGCCGGGCAAATTCCGGCATGGCGGGGCCCGGGTCAACAAAGCCGTTTTCCACGTTGTTCTGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCgg                                                                                                                         <  1:271472/149‑1 (MQ=255)
                                ggCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTg                                                                                                                       <  1:286025/148‑1 (MQ=255)
                                ggCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGa                                                                                                                      <  2:36787/149‑1 (MQ=255)
                                     ggtggtGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGt                                                                                                                 <  7:310520/149‑1 (MQ=255)
                                            cacaACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGt                                                                                                                        >  6:141668/1‑135 (MQ=255)
                                            cacaACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGt                                                                                                                        <  5:141668/135‑1 (MQ=255)
                                             acaACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGtttt                                                                                                         <  3:272104/149‑1 (MQ=255)
                                                aCCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGcc                                                                                                      <  5:43010/149‑1 (MQ=255)
                                                aCCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGGGACGAGTGCAGTTTTGcc                                                                                                      <  1:41126/149‑1 (MQ=255)
                                                  cTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACATGGGCGATGCCCAGGCCAATGCCGCTGGTGGACCCGGTGACGAGTGCAGTTTTGCCtt                                                                                                    >  2:236028/1‑149 (MQ=255)
                                                          cGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGt                                                                                             >  3:112195/1‑148 (MQ=255)
                                                            tGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGt                                                                                                                        >  5:97282/1‑119 (MQ=255)
                                                            tGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGt                                                                                                                        <  6:97282/119‑1 (MQ=255)
                                                             gCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGt                                                                                                                        <  5:89464/118‑1 (MQ=255)
                                                             gCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGt                                                                                                                        >  6:89464/1‑118 (MQ=255)
                                                             gCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATg                                                                                         >  8:63043/1‑149 (MQ=255)
                                                              ccGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATg                                                                                         >  6:119885/1‑148 (MQ=255)
                                                                  gCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATg                                                                                    >  6:325821/1‑149 (MQ=255)
                                                                      tttCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGAc                                                                                                                     >  5:218548/1‑112 (MQ=255)
                                                                      tttCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGAc                                                                                                                     <  6:218548/112‑1 (MQ=255)
                                                                      tttCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTcc                                                                                >  2:303255/1‑149 (MQ=255)
                                                                                      gCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTg                                                                <  5:119885/149‑1 (MQ=255)
                                                                                         cGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGACCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCAt                                                                                          >  4:257896/1‑120 (MQ=255)
                                                                                         cGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGACCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCAt                                                                                          <  3:257896/120‑1 (MQ=255)
                                                                                          gggTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGt                                                            <  1:20181/149‑1 (MQ=255)
                                                                                                 cAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGt                                                     >  6:236351/1‑149 (MQ=255)
                                                                                                 cAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGt                                                     >  5:16659/1‑149 (MQ=255)
                                                                                                    aGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGc                                                              >  6:147025/1‑137 (MQ=255)
                                                                                                    aGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGc                                                              <  5:147025/137‑1 (MQ=255)
                                                                                                        gTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTgg                                                   >  6:103768/1‑144 (MQ=255)
                                                                                                        gTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTgg                                                   <  5:98825/144‑1 (MQ=255)
                                                                                                        gTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTgg                                                   <  5:103768/144‑1 (MQ=255)
                                                                                                        gTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTgg                                                   >  6:98825/1‑144 (MQ=255)
                                                                                                              cACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTg                                                                <  1:140217/125‑1 (MQ=255)
                                                                                                              cACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTg                                                                >  2:140217/1‑125 (MQ=255)
                                                                                                                 gATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTGGCGATGACCACGaa                                      >  7:63417/1‑148 (MQ=255)
                                                                                                                            gcCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTGGCGATGACCACGAACACCGCCAgg                            <  5:325821/149‑1 (MQ=255)
                                                                                                                                   cGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTGGCGATGACCACGAacac                                   >  4:217351/1‑135 (MQ=255)
                                                                                                                                   cGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTGGCGATGACCACGAacac                                   <  3:217351/135‑1 (MQ=255)
                                                                                                                                    gggCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTGGCGATGACCACGAACACCGCCAGGGTCTTGAt                    <  5:316830/149‑1 (MQ=255)
                                                                                                                                         aaTACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTGGCGATGACCACGAACACCGCCAGGGTCTTGATCAggg               >  4:78288/1‑149 (MQ=255)
                                                                                                                                         aaTACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTGGCGATGACCACGAACACCGCCAGGGTCTTGATCAggg               <  4:112195/149‑1 (MQ=255)
                                                                                                                                           tACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAg                                                                                                >  4:281589/1‑66 (MQ=255)
                                                                                                                                           tACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAg                                                                                                <  3:281589/66‑1 (MQ=255)
                                                                                                                                           ggccTGGGCATCGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTGGCGATGACCACGAACACCGCCAGGGTCTTGATCAGGGt              >  6:76240/3‑148 (MQ=255)
                                                                                                                                             ccTGGGCATCGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTGGCGATGACCACGAACACCGCCAGGGTCTTGATCAGGGTaa            >  8:323163/1‑148 (MQ=255)
                                                                                                                                               tGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTGGCGATGACCACGAACACCGCCAGGGTCTTGATCAGGGTAATAc         >  6:8067/1‑149 (MQ=255)
                                                                                                                                                      tGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTGGCGATGACCACGAACACCGCCAGGGTCTTGATCAGGGTAATACCAAAAAt  <  1:303255/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
AAAGCCTCGATCTGGACCACATCCGACAGGTCGGCCGGGTGGTGCACAACCTTCACCCCGTGCCGGGCAATTTCCGCCATGGCGGGGCCCGGGTCACCAAAGCCGTTGAGCACGATGT‑‑TGGCGCCAGCCCGGGCCAATACCTGGGCGATGCCCAGGCCAATGCCGCTGGTGGAACCGGTGACGAGTGCAGTTTTGCCTTCAAGGGTCATGCAATGCTCCTTAGACGATGCCAGTGGCGTAGAACGTGGCGATGACCACGAACACCGCCAGGGTCTTGATCAGGGTAATACCAAAAAT  >  NC_002947/3457080‑3457376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑