Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 321,041 C→G 7.1% C145W (TGC→TGG)  aguA → agmatine deiminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947321,0410CG 7.1% 178.0 / 4.2 56C145W (TGC→TGG) aguAagmatine deiminase
Reads supporting (aligned to +/- strand):  ref base C (26/26);  new base G (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

TACCTTCGTCATCAACGACCATGGCGAAGTGCGCGGTGTAGATTGGGGCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGCCTGCTCAACCGCAACCGCAACCCGCACCTGAGCCGCGAGCAGATTGAAGCGGTGCTGC  >  NC_002947/320900‑321186
                                                                                                                                             |                                                                                                                                                 
tACCTTCGTCATCAACGACCATGGCGAAGTGCGCGGTGTAGATTGGGGCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAgcgc                                                                                                                                             >  6:215099/1‑148 (MQ=255)
       gTCATCAACGACCATGGCGAAGTGCGCGGTGTAGATTGGGGCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGAc                                                                                                                                     <  6:330433/149‑1 (MQ=255)
         catcaACGACCATGGCGAAGTGCGCGGTGTAGATTGGGGCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGTAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGGCAGCGCtccat                                                                                                                                        <  8:179991/144‑5 (MQ=255)
         catcaACGACCATGGCGAAGTGCGCGGTGTAGATTGGGGCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGGCAGCGCtccat                                                                                                                                        >  7:179991/1‑140 (MQ=255)
           tcaACGACCATGGCGAAGTGCGCGGTGTAGATTGGGGCTTCAACGCCTGGGGAGGCTTCGATGGCGGCCTGTACGCAACCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTAGCAGACCg                                                                                                                                   >  8:312476/1‑147 (MQ=255)
                       gCGAAGTGCGCGGTGTAGATTGGGGCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTg                                                                                                                     >  2:35431/1‑149 (MQ=255)
                         gAAGTGCGCGGTGTAGATTGGGGCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGa                                                                                                                   <  8:26881/149‑1 (MQ=255)
                         gAAGTGCGCGGTGTAGATTGGGGCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGa                                                                                                                   <  8:21814/149‑1 (MQ=255)
                         caactGCGCGGGCTCGCTTGGGGCTGCAACGCCGGGGGCGGCTTCGATGGCGGCCGGCACCCACCCGGGAACCGCGACGAGCAACCGGCCGCCAAGGTGCTCGCAATGGAGCCCTGCCACCGCTACCAGACCGCAGCCCTTGCGCTGGa                                                                                                                   <  8:220677/145‑1 (MQ=255)
                          aaGTGCGCGGTGTAGATTGGGGCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGaa                                                                                                                  <  1:35431/149‑1 (MQ=255)
                            gTGCGCGGTGTAGATTGGGGCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAgg                                                                                                                >  8:3364/1‑149 (MQ=255)
                                 cgGTGTAGATGGGGGCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGtt                                                                                                           <  5:326774/149‑1 (MQ=255)
                                         atgggggCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCAc                                                                                                   <  7:234080/146‑1 (MQ=255)
                                         aTTGGGGCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCAc                                                                                                   <  8:76765/149‑1 (MQ=255)
                                         aTTGGGGCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCGGGAAGGTGGTTCGATCCAc                                                                                                   <  5:113811/149‑1 (MQ=255)
                                           tGGGGCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACg                                                                                                  >  2:260960/1‑148 (MQ=255)
                                             gggCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACg                                                                                                  >  4:22312/1‑146 (MQ=255)
                                                cTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCAGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACg                                                                                            >  6:125894/1‑149 (MQ=255)
                                                   cAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGt                                                                                          <  3:22312/148‑1 (MQ=255)
                                                     aCGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTg                                                                                         >  4:65575/1‑147 (MQ=255)
                                                                   ttCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGaa                                                                                                                                 >  6:231392/1‑93 (MQ=255)
                                                                   ttCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGaa                                                                                                                                 <  5:231392/93‑1 (MQ=255)
                                                                   ttCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATcac                                                                         >  2:149378/1‑149 (MQ=255)
                                                                      gATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATcaccac                                                                      <  7:3364/149‑1 (MQ=255)
                                                                       aTGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGAt                                                                                                       >  5:60712/1‑115 (MQ=255)
                                                                       aTGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGAt                                                                                                       <  6:60712/115‑1 (MQ=255)
                                                                       aTGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACt                                                                     >  1:36268/1‑149 (MQ=255)
                                                                           cggcCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACgg                                                                                           >  2:31328/1‑123 (MQ=255)
                                                                           cggcCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGAGCTGGAAGGTGGTTCGATCCACGTAGACgg                                                                                           <  1:31328/123‑1 (MQ=255)
                                                                              ccTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAAt                                                              <  1:260960/149‑1 (MQ=255)
                                                                              ccTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAAt                                                              <  5:215099/149‑1 (MQ=255)
                                                                                tGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGc                                                            >  5:311016/1‑149 (MQ=255)
                                                                                  tACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGc                                                            <  2:36268/147‑1 (MQ=255)
                                                                                    cGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGCCTGc                                                        <  6:311016/149‑1 (MQ=255)
                                                                                         ccTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGCCCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTACACGGTGAAGGCCCCGTGATCACCACTGACGAATGCCTGCTcaac                                                   >  7:49864/1‑149 (MQ=255)
                                                                                         ccTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATcacca                                                                       >  6:71745/1‑129 (MQ=255)
                                                                                         ccTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATcacca                                                                       <  5:71745/129‑1 (MQ=255)
                                                                                         ccTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGCCTGCTcacc                                                   >  2:191984/1‑147 (MQ=255)
                                                                                            ggAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGGGGTTCGCTCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGCCTGCTcaaccgc                                                >  2:207847/1‑149 (MQ=255)
                                                                                                         ggAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATc                                                                                                      <  4:277704/82‑1 (MQ=255)
                                                                                                         ggAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATc                                                                                                      >  3:277704/1‑82 (MQ=255)
                                                                                                         ggAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGCCTGCTCAACCGCAACCGCAACCCg                                    >  2:155942/1‑148 (MQ=255)
                                                                                                          gAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGc                                                                                    <  4:165186/99‑1 (MQ=255)
                                                                                                          gAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGc                                                                                    >  3:165186/1‑99 (MQ=255)
                                                                                                            aCTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGCCTGCTcaaccgcaaccg                                           <  3:156845/138‑1 (MQ=255)
                                                                                                            aCTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGCCTGCTcaaccgcaaccg                                           >  4:156845/1‑138 (MQ=255)
                                                                                                             cTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGCCTGCTCAACCGCAACCGCAACCCGCACCt                               <  3:65575/149‑1 (MQ=255)
                                                                                                                gccgccAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCg                                                                                >  7:326025/1‑97 (MQ=255)
                                                                                                                gccgccAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCg                                                                                <  8:326025/97‑1 (MQ=255)
                                                                                                                gccgccAAGGTGCTGGAAATGGAGCGCTGCAAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGCCTGCTCAACCGCAACCGCAACCCGCACCTGa                             <  7:312476/148‑1 (MQ=255)
                                                                                                                               gAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGCCTGCTCAACCGCAACCGCAACCCGCACCTGAGCCGCGAGCAGATTg              >  5:342961/1‑148 (MQ=255)
                                                                                                                                aaaTGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGCCTGCTCAACCGCAACCGCAACCCGCACCTGAGCCGCGAGCAGATTGaa            <  1:149378/149‑1 (MQ=255)
                                                                                                                                  aTGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGCCTGCTCAACCGCAACCGCAACCCGCACCTGAGCCGCGAGCAGATTGAAGc          >  8:272741/1‑149 (MQ=255)
                                                                                                                                    gtaGCGCTGGCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGCCTGCTCAACCGCAACCGCAACCCGCACCTGAGCCGCGAGCAGATTGAAGCgg        >  6:193646/3‑149 (MQ=255)
                                                                                                                                    gtaGCGCTGGCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGCCTGCTCAACCGCAACCGCAACCCGCACCTGAGCCGCGAGCAGATTGAAGCgg        <  5:193646/147‑1 (MQ=255)
                                                                                                                                          cTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGCCTGCTCAACCGCACACGCAACCCGCACCTGAGCCGCGAGCAGCTTGAAGCGGtgcggc  >  7:79983/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
TACCTTCGTCATCAACGACCATGGCGAAGTGCGCGGTGTAGATTGGGGCTTCAACGCCTGGGGCGGCTTCGATGGCGGCCTGTACGCACCCTGGAACCGCGACGAGGAACTGGCCGCCAAGGTGCTGGAAATGGAGCGCTGCCAGCGCTACCAGACCGAAGGCTTTGTGCTGGAAGGTGGTTCGATCCACGTAGACGGTGAAGGCACCGTGATCACCACTGAGGAATGCCTGCTCAACCGCAACCGCAACCCGCACCTGAGCCGCGAGCAGATTGAAGCGGTGCTGC  >  NC_002947/320900‑321186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑