Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,559,861 C→T 7.3% G80D (GGC→GAC)  PP_3143 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,559,8610CT 7.3% 159.0 / 2.5 55G80D (GGC→GAC) PP_3143LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (25/26);  new base T (2/2);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

CAGGCGCAACTCGATGTCCGGGTAACGTTGTTGAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATCTGCTGGCTGACCGCGCCCGGGGTGATGTGCAGCTC  >  NC_002947/3559713‑3560009
                                                                                                                                                         |                                                                                                                                                    
caGGCGCAACTCGATGTCCGGGTAACGTTGTTGAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGc                                                                                                                                                      >  4:202474/1‑149 (MQ=255)
     gcAACTCGATGTCCGGGTAACGTTGTTGAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAg                                                                                                                                                 >  4:113999/1‑149 (MQ=255)
       aaCTCGATGTCCGGGTAACGTTGTTGAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTa                                                                                                                                                  >  1:62388/1‑146 (MQ=255)
       aaCTCGATGTCCGGGTAACGTTGTTGAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTa                                                                                                                                                  <  2:62388/146‑1 (MQ=255)
        aCTCGATGTCCGGGTAACGTTGTTGAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGcc                                                                                                                                              >  7:116257/1‑149 (MQ=255)
         cTCGATGTCCGGGTAACGTTGTTGAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGcat                                                                                                                                             >  2:275340/1‑147 (MQ=255)
          tCGATGTCCGGGTAACGTTGTTGAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTc                                                                                                                                            >  6:16086/1‑149 (MQ=255)
           cGATGTCCGGGTAACGTTGTTGAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCg                                                                                                                                           >  8:38734/1‑149 (MQ=255)
            gATGTCCGGGTAACGTTGTTGAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCgg                                                                                                                                          <  6:199045/149‑1 (MQ=255)
            gATGTCCGGGTAACGTTGTTGAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCgg                                                                                                                                          <  4:102781/149‑1 (MQ=255)
                tCCGGGTATCGTTGTTGAATACGCGGCAAGCGCGGCGTGAGCCATTTGGCGTGCACTAAGTAGTCGGGTTTCATCGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATAGTGCCGTAGGCCTCg                                                                                                                                           <  1:284139/149‑1 (MQ=255)
                  cGGGTAACGTTGTTGAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCg                                                                                                                                    >  8:124827/1‑149 (MQ=255)
                    ggTAACGTTGTTGAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTc                                                                                                                                  >  5:135904/1‑149 (MQ=255)
                          gttgttGAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGc                                                                                                                             >  6:219443/1‑148 (MQ=255)
                           ttgttgAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGc                                                                                                                             >  5:354292/1‑147 (MQ=255)
                           ttgttgAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCa                                                                                                                           <  8:116257/149‑1 (MQ=255)
                              ttgAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGc                                                                                                                        <  5:219443/149‑1 (MQ=255)
                                   aaCGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGc                                                                                                                   >  5:286934/1‑149 (MQ=255)
                                    aCGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCACCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGTCGTAGGCCTCGGCCACCGTCAGGGCCAGGCGTTGcc                                                                                                                  >  4:55417/1‑149 (MQ=255)
                                     cgcgGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGccc                                                                                                                 <  3:125326/149‑1 (MQ=255)
                                         gCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCTGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCg                                                                                                             <  4:223295/149‑1 (MQ=255)
                                           aaGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGgc                                                                                                           <  6:4065/149‑1 (MQ=255)
                                               gcggcgTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTc                                                                                                       <  8:190801/149‑1 (MQ=255)
                                                  gcgTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGc                                                                                                    <  5:237794/149‑1 (MQ=255)
                                                    gTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTc                                                                                                  <  6:135904/149‑1 (MQ=255)
                                                          cATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCAcgcc                                                                                            <  3:68979/149‑1 (MQ=255)
                                                                               acgGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTCGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAg                                                                             >  8:165548/1‑148 (MQ=255)
                                                                                  ggagtgAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAAc                                                                         <  8:141467/144‑1 (MQ=255)
                                                                                        aaCGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCgg                                                                   >  4:74455/1‑149 (MQ=255)
                                                                                          cGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTCCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTa                                                                 <  7:38734/149‑1 (MQ=255)
                                                                                             acacGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGGCCCTCGGCGCTCAGCTCCACGCCGCGCGccc                                                                                    <  4:307013/127‑1 (MQ=255)
                                                                                             acacGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGGCCCTCGGCGCTCAGCTCCACGCCGCGCGccc                                                                                    >  3:307013/1‑127 (MQ=255)
                                                                                             acacGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGcc                                                              >  3:205113/1‑149 (MQ=255)
                                                                                                 gCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAgg                                                          >  3:105985/1‑149 (MQ=255)
                                                                                                    aTGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGgcgc                                                       <  3:202474/149‑1 (MQ=255)
                                                                                                       tgCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGctc                                                     <  3:113999/148‑1 (MQ=255)
                                                                                                           gcgGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCa                                                <  7:124827/149‑1 (MQ=255)
                                                                                                           gcgGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCa                                                <  5:16086/149‑1 (MQ=255)
                                                                                                                      ccGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATc                                     >  6:132142/1‑149 (MQ=255)
                                                                                                                      ccGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATc                                     >  1:180705/1‑149 (MQ=255)
                                                                                                                       cgcgTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGgc                                          >  2:336052/1‑143 (MQ=255)
                                                                                                                       cgcgTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGgc                                          <  1:336052/143‑1 (MQ=255)
                                                                                                                       cgcgTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTCGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATct                                    <  7:165548/149‑1 (MQ=255)
                                                                                                                           tCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATctgctg                                <  3:74455/149‑1 (MQ=255)
                                                                                                                            ccAGCCGCAGCAGCTCGGCTTCGATACTGTCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATCTgctgg                               >  1:153359/1‑149 (MQ=255)
                                                                                                                            ccAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCtt                                                   <  4:203275/129‑1 (MQ=255)
                                                                                                                            ccAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCtt                                                   >  3:203275/1‑129 (MQ=255)
                                                                                                                               gCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATCTgctggct                             >  3:95944/1‑148 (MQ=255)
                                                                                                                                  gcagcaGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATCTGCTGGCTGccc                         >  4:307717/1‑149 (MQ=255)
                                                                                                                                            ggCTTCGATACTGTCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATCTGCTGGCTGACCGCGCCCggg                <  2:153359/148‑1 (MQ=255)
                                                                                                                                              cTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATCTGCTGGCTGACCGCGCCCGGGGt              >  6:181164/1‑148 (MQ=255)
                                                                                                                                                  gATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATCTGCTGGCTGACCGCGCCCGGGGTGAtgt         <  5:181164/149‑1 (MQ=255)
                                                                                                                                                    tACTGTCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATCTGCTGGCTGACCGCGCCCGGGGTGATGTGc       <  3:55417/149‑1 (MQ=255)
                                                                                                                                                    tACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATCTGCTGGCTGACCGCGCCCGGGGTGAtgtg        >  6:223283/1‑148 (MQ=255)
                                                                                                                                                     aCTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATCTGCTGGCTGACCGCGCCCGGGGTGATGTGCa      >  3:85280/1‑149 (MQ=255)
                                                                                                                                                      cTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATCTGCTGGCTGACCTCGCCCGGGGTGATGTGCa      <  2:180705/148‑1 (MQ=255)
                                                                                                                                                         ccGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATCTGCTGGCTGACCGCGCCCGGGGTGATGTGCAGctc  >  7:150759/1‑149 (MQ=255)
                                                                                                                                                         ccGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATCTGCTGGCTGACCGCGCCCGGGGTGATGTGCAGctc  >  1:145656/1‑149 (MQ=255)
                                                                                                                                                         |                                                                                                                                                    
CAGGCGCAACTCGATGTCCGGGTAACGTTGTTGAAAACGCGGCAAGCGCGGCGTGAGCCATTTGGC‑‑‑CAGGAA‑‑CGACGGGATCGAACGCACACGCAATGTGCCGCGGATTTCCCCCGCGTCCAGCCGCAGCAGCTCGGCTTCGATACTGCCGTAGGCCTCGGCCACCGTCTGGGCCAGGCGTTGCCCCTCGGCGCTCAGCTCCACGCCGCGCGCCCGGCGCAGGAACAGCCGGTAGCCCAGGCGCTCTTCCAGTTGGCGCATCTGCTGGCTGACCGCGCCCGGGGTGATGTGCAGCTC  >  NC_002947/3559713‑3560009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑