| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,740,819 | 2 bp→CT | 100% | intergenic (+56/+696) | gltA → / ← yeiW | citrate synthase/putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,740,819 | 0 | T | C | 95.9% | 142.2 / ‑2.3 | 48 | intergenic (+56/+697) | gltA/yeiW | citrate synthase/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (27/19); total (28/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_002947 | 4,740,820 | 0 | G | T | 100.0% | 153.6 / ‑6.8 | 48 | intergenic (+57/+696) | gltA/yeiW | citrate synthase/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (27/19); total (28/20) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACAC‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCA > NC_002947/4740677‑4740954 || tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACAC‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg > 6:342455/1‑149 (MQ=255) cccGTACAAGATCGGCCGTCCGCGCCAGCTGTACAC‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCT‑GTGCCGGCctct > 6:144511/1‑149 (MQ=255) ccGTACAAGATCGGCCGTCCGCGCCAGCTGTACAC‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct < 6:13883/149‑1 (MQ=255) aCAAGATCGGCCGTCCGCGCCAGCTGTACAC‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc > 7:102713/1‑149 (MQ=255) cAAGATCGGCCGTCCGCGCCAGCTGTACACGCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGCCCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGATGCCGGCGGGCCGCC‑TTTTTTATTTTTCCTGTGCCGGCCTCTTcgc > 1:205637/1‑149 (MQ=255) gATCGGCCGTCCGCGCCAGCTGTACAC‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGcc < 6:162239/149‑1 (MQ=255) ccGTCCGCGCCAGCTGTACAC‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGccc > 7:216544/1‑149 (MQ=255) ccGTCCGCGCCAGCTGTACAC‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAG‑CGTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGccc > 5:298903/1‑149 (MQ=255) cGTCCGCGCCAGCTGTACAC‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCcg < 8:102713/149‑1 (MQ=255) cgcgCCAGCTGTACAC‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgcg > 6:105525/1‑135 (MQ=255) cgcgCCAGCTGTACAC‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgcg < 5:105525/135‑1 (MQ=255) cgcgCCAGCTGTACAC‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑Gaa < 8:206861/149‑1 (MQ=255) cgcgCCAGCTGTACAC‑CGGCGAGAAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTACTGTGCCGGCCACATCGCGGCCATGACCAC‑Ga > 7:216171/1‑148 (MQ=255) agcCAGCTGTACAC‑CGTCTAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTACGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAaga < 8:216544/148‑1 (MQ=255) cAGCTGTACAC‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCTTAGCGGGGATGGCCGC‑Gaaccggg > 1:336142/1‑144 (MQ=255) tACAC‑GGGCTACCATAAGCGGCACATCGTTGCCCTTAAACACCAAAATTATGTTGCATTCCCCTAACCAAATACGGCGGCCCTCGGGCTGCTTTTTTTTTTTGTCCTGG‑GCCGGCCTCTTCGCGGCCTTGCCCGC‑TATGTGGCCGGca < 6:298903/148‑1 (MQ=255) tACAC‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGca < 5:342455/148‑1 (MQ=255) ac‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAgg < 8:37483/139‑1 (MQ=255) ac‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCAggtcggaa > 7:37483/1‑143 (MQ=255) ggCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGacac > 6:285397/1‑149 (MQ=255) ggCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGacac < 5:285397/149‑1 (MQ=255) gAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGAcacca > 3:294558/1‑148 (MQ=255) gACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCAGtt > 1:33037/1‑149 (MQ=255) cccTGAAAGACCGCAAGTACGCTGCAGTAGCCGACCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTGCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcc < 2:205637/149‑1 (MQ=255) aCCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGc > 8:186216/1‑149 (MQ=255) cAAGTAAGCTGCAGTAGCCTAACGCAAGAAGGCTGCCGTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGTCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGcc < 7:84080/149‑1 (MQ=255) gTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACCGACACCACAAAACCACAGTTTTTCTCCGCCCGCTCACGCAccccgt > 6:282645/1‑147 (MQ=255) gTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAtt > 2:226838/1‑149 (MQ=255) gCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGTCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg < 1:162278/141‑1 (MQ=255) gCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCATGCCCCC‑GAAGAGGGGGGCACACACACCACAAAACCACACTTTTTATACGCCCCCTCACCCAGCCCATTAAgggagatcgg > 2:162278/1‑142 (MQ=255) aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc > 1:210796/1‑149 (MQ=255) aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGGTTTGAACGGCGCATc > 5:81386/1‑149 (MQ=255) aacacggCTGCCGTC‑GGCAAGCTTTTTTTATTTGTCCTGGCCCGCCCTCTCCGCGCCCTGGCCCGC‑TATGTGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc < 5:43071/144‑1 (MQ=255) ccaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTCCCGCCCCCTTCGCGGCCTTCCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTTAACGGCGCATcc < 4:225903/147‑1 (MQ=255) aagGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCa > 2:203670/1‑95 (MQ=25) aagGCTGCCCGAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCa < 1:203670/95‑1 (MQ=25) gCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCcaccac < 4:294558/149‑1 (MQ=255) tGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCACACGCAGCCCTTTCAGGGTATTGAAAGGCGCATCAACAACg > 4:56901/1‑148 (MQ=255) cccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑TAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGaa < 3:56901/148‑1 (MQ=255) ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACGTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCAACAACGAAca > 3:187322/1‑148 (MQ=255) ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACt > 6:337407/1‑149 (MQ=255) gggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑TAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt < 5:337407/149‑1 (MQ=255) gggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt < 2:33037/149‑1 (MQ=255) gCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACCCCACAAAACCTCAGTTTTTATGCGCCCGCTCACGCAGCCCTGTCAGGGTATTGAACGGCGGATCGACCACGCACTTGTTgg > 3:295066/1‑149 (MQ=255) gccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCGCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGcca > 8:76657/4‑149 (MQ=255) ccttttttATTTGTCCTGTGCCGGCCTCTTGGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCCGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccac > 1:31335/3‑149 (MQ=255) ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccac > 1:71266/1‑148 (MQ=255) ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccacca > 5:186285/1‑149 (MQ=255) || TCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACAC‑CGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGC‑GAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCA > NC_002947/4740677‑4740954 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |