| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | 100% | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 97.1% | 109.3 / ‑6.2 | 39 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base A (19/18); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 100.0% | 117.0 / NA | 39 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (21/18); total (21/18) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑T‑CGGGCA‑CG‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCT > NC_002947/1499339‑1499596 || cGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCtt > 2:242070/1‑149 (MQ=255) ggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCTGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGTACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCTCGGTGCATGGCACCGGCTTTGCCg < 2:297115/149‑1 (MQ=38) ttCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCCAAAAACGCGCCCCGGTGCCATGCCCCCGCTTTGCCCGTGttacc > 8:151225/1‑146 (MQ=255) ctaagggagtcatgattcgttagcgaggcaacaaaaaacgggccgtcactgtagcgataggcccgcttaatggcaatcagtctGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGACACCGGCTTTGCCGgtgttcgc > 6:203978‑M1/84‑145 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgg < 7:250718/148‑6 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGCGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATAGCACCGGCTTTGCCGGTGttcgcgg < 7:223159/148‑6 (MQ=37) agggagtcatgattcgttagcgaggcaacaaaaaacgggccgtcactgtagcgataggcccgcttaatngcaatcagtctGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCggtgttcgcg > 1:109984‑M1/81‑142 (MQ=255) agggagtcatgattcgttagcgaggcaacaaaaaacgggccgtcactgtagcgataggcccgcttaatggcaatcagtctGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCggtgttcgcg > 1:113893‑M1/81‑142 (MQ=255) agggaggcatgattcgttagcgaggcaacaaaaaacgggccgtcactgtagcgataggcccgcttaatggcaatcagtctGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCggtgttcgcg > 3:201237‑M1/81‑142 (MQ=255) aCAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcggg > 4:112071/1‑142 (MQ=255) ggagtcatgattcgttagcgaggcaacaaaaaacgggccgtcactgtagcgataggcccgcttaatggcaatcagtctGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGc > 4:4105‑M1/79‑133 (MQ=255) ggagtcatgattcgttagcgaggcaacaaaaaacgggccgtcactgtagcgataggcccgcttaatggcaatcagtctGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGc < 3:4105‑M1/55‑1 (MQ=255) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGc > 6:275489/1‑121 (MQ=255) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGc < 5:275489/121‑1 (MQ=255) cccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAACAGGCGCCGCGGTGCATGGCACCGGCGTTGCCGGTGTTAG‑CGGGCC‑cg‑cccgcgccc > 8:77785/1‑142 (MQ=25) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGCGGCTGCCAAACGGTGGGAGCGGGCGTGCCCCCGAAACACGCGCCCCGGTGCAAGGCCCCCGCGTTGCCCGTGTTAG‑CGGGCA‑CG‑cc‑cccccccc > 3:132613/1‑146 (MQ=255) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgc < 3:206719/131‑4 (MQ=255) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgc > 4:206719/1‑128 (MQ=255) gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgg < 7:290230/124‑6 (MQ=255) gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgcgg > 8:290230/1‑119 (MQ=255) gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCC‑GCTCCCACAGACTGATTGCCATTAAGc < 3:49821/149‑1 (MQ=21) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGc < 7:77785/148‑1 (MQ=21) aaCGAAGCCTTGCCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCC‑GCTCCCACAGACTGATTGCCATTAAGCggg < 3:216054/147‑1 (MQ=21) cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgc < 5:40686/105‑4 (MQ=255) cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGttcgc > 6:40686/1‑102 (MQ=255) gCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGGGAAACAGGCGCCCCGGGGGATGGCACCGGCTTTGCCGGTGTTAG‑CGGGCC‑CGCCC‑GCGCCCACAGAATGCATGCCATTAAACGGGCCAAtagg > 6:262141/1‑146 (MQ=18) ggCACCACAGCAGGAGTGGGGCTGCCAAAGGGTGGGAGGGGGGGTGCCCGCTAAACTGGCGCGGGGGTGCATGGCACGGGCTTTGCGGGTGTTCG‑CGGGCA‑CGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa < 4:132613/148‑1 (MQ=21) ggCACCACAGCAGGACTGTGGCGGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCAGGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa < 3:63644/148‑1 (MQ=21) ggCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCACCC‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACa < 1:242070/148‑1 (MQ=21) gCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGt < 3:112071/149‑1 (MQ=21) agcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCC‑CGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGcc > 7:235036/1‑149 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 1:228925/147‑1 (MQ=21) ccAAACGGTGGGAGCGGGCGTGCACGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCC‑GCTCCCACAGACTGATTGCCa > 7:310694/1‑103 (MQ=14) ccAAACGGTGGGAGCGGGCGTGCACGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCC‑GCTCCCACAGACTGATTGCCa < 8:310694/103‑1 (MQ=14) tagggggCGTTCCCGCGAAACAGGCGCCGCGGTGCAGGGCACGGGCTTTGCCGGTGTTCG‑GGGGAA‑CGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc < 4:147555/145‑1 (MQ=255) GAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCA‑CG‑CCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc > 8:255317‑M1/1‑149 (MQ=255) GCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCA‑CG‑CCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt > 7:151225‑M1/1‑149 (MQ=255) CGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCA‑CG‑CCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATg > 3:220638‑M1/1‑149 (MQ=255) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCA‑CGCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 1:253442/1‑147 (MQ=37) || CGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑T‑CGGGCA‑CG‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCT > NC_002947/1499339‑1499596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |