| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 97.5% | 135.1 / NA | 40 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (16/23); minor base G (0/1); total (16/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAT > NC_002947/1932503‑1932779 | cGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAACCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGc > 3:317886/1‑148 (MQ=255) gAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCt < 2:45194/148‑1 (MQ=255) ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGTATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACt < 5:173483/148‑1 (MQ=255) aTTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGAGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTAcgc > 1:74747/1‑149 (MQ=255) aTTGCAGGGCTCACCAAAAACCCAGTAACCGCTGGGGCCGCTTGGCGGCCCTTCGCGGCCAAGCCCGCTCCTACAAAGGATAGGCGCCCCCTGTCGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTAcgc < 1:247793/149‑1 (MQ=37) ctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCGGTAGGAGCGGGCTTGC‑CCGCGAAGGGCAGCAAGGCAGCCCCA‑AATTGTCGACTAACCGGATCAGc > 7:199928/1‑148 (MQ=37) ccAGTAACCGGTGGGGCGGCTTTGCGGCCCTTCGGGGGCATGCCCGAGCCTCAACAGGATTTGCTCCCCGTGACGCTGCGGGGTGGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAggg < 8:191072/147‑1 (MQ=255) cAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGt > 2:53358/1‑148 (MQ=255) tAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCGACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAg > 4:240345/1‑142 (MQ=255) aaaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAgg < 3:240345/142‑1 (MQ=255) aggCTGGGGCCCGTTTGGGGCCGGTGGCGGGAAAGCCGTCTCCTACAACGGATATGGGTCCCTTGTTGGAGGGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc < 2:125091/147‑1 (MQ=25) cTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGGTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGc < 8:104796/149‑1 (MQ=255) ggCCGCTTTGCGGCCCTTCGCGGGCAAACCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCt < 4:317886/146‑1 (MQ=255) cTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑ATTTGTCGACTACGCGGCTCAGCGATGCGGCAGGGGGATGGTCAGCTCCAGCATc > 1:332681/1‑149 (MQ=37) tttCCGCCCCTTCGCGGGCACGCCCGCTCCTACAAAGTATATGCGGCCCCTTTAGTAGGGGGCTGGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc < 4:23131/148‑1 (MQ=255) gCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGa < 6:361381/147‑1 (MQ=255) cGGCCGTTCGGGGGAACGCCCGCTCCTAC‑AAGGTTATTGGTTCCCGTTATGTTGGGGGTTGGCCCGAGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCTGATCAGCGATCCGGCAGTGTGATGTTCAGCTCCAGAATCGagc < 1:212385/148‑1 (MQ=255) ggCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGaa > 2:306664/1‑141 (MQ=255) ggCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGaa < 1:306664/141‑1 (MQ=255) gcgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGAt > 2:130553/1‑99 (MQ=255) gcgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGAt < 1:130553/99‑1 (MQ=255) gcgGCCAAGCCCGCTCCAACAAAGGATATGCGTCCCCTGTCGGCGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTg < 2:332681/149‑1 (MQ=39) gCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGttt > 1:300586/1‑149 (MQ=255) aGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc < 1:53358/149‑1 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata < 2:74747/149‑1 (MQ=255) tACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat < 5:351698/147‑1 (MQ=255) aCAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCATGATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat > 1:326408/1‑149 (MQ=39) aCAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCa < 8:88326/139‑1 (MQ=255) aCAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCa > 7:88326/1‑139 (MQ=255) aCAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGatat > 5:89838/1‑148 (MQ=255) tatGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAg < 8:9710/87‑1 (MQ=255) tatGCGTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAg > 7:9710/1‑87 (MQ=255) tGCGTCCCCTCTCGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCg < 2:284928/149‑1 (MQ=255) gTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc < 2:155170/104‑1 (MQ=255) gTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc > 1:155170/1‑104 (MQ=255) gTCCCCTGTAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt < 6:89838/148‑1 (MQ=255) tAGGAGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGtt < 7:88653/149‑1 (MQ=255) aGCGGGCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTcaca > 1:71643/1‑149 (MQ=255) gggCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACatat < 2:300586/149‑1 (MQ=255) gCTTGC‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagc > 5:99058/1‑91 (MQ=255) | CGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑‑CCGCGAAGGGCTGCAAGGCAGCCCCA‑AATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAT > NC_002947/1932503‑1932779 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |