Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 2,921,460 G→A G3494R (GGG→AGG)  PP_2561 → hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,921,4600GA97.8% 145.0 / ‑5.0 45G3494R (GGG→AGG) PP_2561hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (23/21);  minor base T (0/1);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

GGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGA  >  NC_002947/2921325‑2921595
                                                                                                                                       |                                                                                                                                       
ggggAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGAGAAGACAGCCTGGATGGAGGCAACGGCAATGACACCCTGAAGGGCGGCGATGGTAACGACACATTGTTCGTCGACGTGGGTACCGACACCATGATAAGGGGggcaggca                                                                                                                             >  6:390723/1‑148 (MQ=255)
   gAGGCCGGTAACGACACCATGAACGCGGGCGATGGCGATGAAAGCCTGGATGGCGGCAACGGAAATGACACCCTGGAGGGCGGCGATGGTAACGACACATTGTTCGGAGACGTGTGTACCGACACCCTGAGCAGGGGGGCCGGCaa                                                                                                                            >  6:199278/1‑146 (MQ=255)
     ggCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGAc                                                                                                                        <  3:51654/148‑1 (MQ=255)
        cGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTc                                                                                                                     >  3:227585/1‑148 (MQ=255)
         ggTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTcc                                                                                                                    >  3:250772/1‑148 (MQ=255)
          gTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCt                                                                                                                   <  4:227585/148‑1 (MQ=255)
               gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACg                                                                                                              >  3:27261/1‑148 (MQ=255)
               gACAACCTGAACGGGGGCGATGGCGATGACAGCCTGGATGGCGGCAACGGCAATCACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGAACAGTGGGCCCGGCAACGACTTCCTCAACg                                                                                                              >  6:16046/1‑148 (MQ=255)
                  aaCCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGc                                                                                                          <  8:224354/149‑1 (MQ=255)
                      tGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGc                                                                                                       <  4:27261/148‑1 (MQ=255)
                       gAACGGGGGCGCTGGCGATGACAGCCAGGATGGCGGCAACGGCAATGACACCCTGCAGGCCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAg                                                                                                     >  7:212720/1‑149 (MQ=255)
                         aCGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGt                                                                                                   <  7:179581/149‑1 (MQ=255)
                             gggCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAAc                                                                                                >  2:183523/1‑148 (MQ=255)
                              ggCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACGCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGa                                                                                              <  2:125072/149‑1 (MQ=255)
                                     gCGATGACAGCCTGGATGGAGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATAGTTCGGCGCCGTGGGTACCGAAACACTGAACAGGGGGGCAGGAAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggt                                                                                        >  4:110246/1‑148 (MQ=255)
                                              gCCGGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTACCGCCACTTTGGTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGtgggtggg                                                                              <  1:309091/149‑1 (MQ=255)
                                               ccTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTgggg                                                                             >  2:107685/1‑149 (MQ=255)
                                                  ggctGGCGGCAACGGCAAAGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGcc                                                                           <  5:390723/145‑1 (MQ=255)
                                                    aTGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCgg                                                                         >  8:61959/1‑148 (MQ=255)
                                                      ggcggcAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTaa                                                                      >  2:72497/1‑149 (MQ=255)
                                                       gcggcAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGTCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTaa                                                                      <  1:352563/148‑1 (MQ=255)
                                                       gcggcAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGTGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTaa                                                                      <  7:66372/148‑1 (MQ=255)
                                                                 caaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATg                                                            >  6:264304/1‑148 (MQ=255)
                                                                   aTGACACCCTGCAGGGCGGCGATGGTAACGACTCATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATc                                                         >  2:366957/1‑149 (MQ=255)
                                                                       cacCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtggg                                                                                  <  7:56738/120‑1 (MQ=255)
                                                                       cacCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtggg                                                                                  >  8:56738/1‑120 (MQ=255)
                                                                       cacCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGc                                                       <  5:264304/147‑1 (MQ=255)
                                                                           cTGCAGGGCGGCGTTGGTACCGACACATTGTTCGGCGACGTCGGTACGGACACCCTGATCTGGGGGGGCGGGTACGACTTCCTAAACGGTTCGGCAGGTATCGATACGGTGGTGGGTGGGGCCGGTTACGACACCATGATCGGCACCGa                                                 <  8:212720/149‑1 (MQ=255)
                                                                              cAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGTTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGAt                                                <  1:107685/147‑1 (MQ=255)
                                                                               aGGGCGGCGATGGTAACGACACATTGTTCGGCGACGGGGGTACCGACACCCTGATCAGGTGGGCCGGCAACGACTGCCTCAACGCTGCGGCAGGGAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGAt                                                <  3:110246/146‑1 (MQ=255)
                                                                                    ggcgATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGa                                        <  1:183523/149‑1 (MQ=255)
                                                                                      cgATGGTAACGACACATTGTTCGGCGACGTTGGTACCTACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGTTAACTATACGGTGGTGGTTGTGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAt                                       <  5:199278/148‑1 (MQ=255)
                                                                                         tGGTTACGTCACATTGTTGGGCGAGGTGGTTACGGACACCCTGATGAGGGGGGCCGGAAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTTGTGGGTGGGGCCTGTAACGACACCATGATCGCCACCGATGGAAACGAtgtg                                    <  5:16046/148‑1 (MQ=255)
                                                                                                     aTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATc                                                         >  8:268035/1‑115 (MQ=255)
                                                                                                     aTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATc                                                         <  7:268035/115‑1 (MQ=255)
                                                                                                        gTTCGGCGACGTGGGTACCGACACACTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAAAGATGTGTTCCAGTTCGCTGCa                     >  6:95348/1‑148 (MQ=255)
                                                                                                         ttCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAgg                   >  1:370303/1‑149 (MQ=255)
                                                                                                                aCGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTgg             <  4:250772/148‑1 (MQ=255)
                                                                                                                 cGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCCATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTgg             >  5:123770/1‑147 (MQ=255)
                                                                                                                      gTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGc                  >  3:26913/1‑137 (MQ=255)
                                                                                                                       tACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCt                 <  4:26913/137‑1 (MQ=255)
                                                                                                                       tACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAcc     >  1:313783/1‑149 (MQ=255)
                                                                                                                          cGACACCCAGATCAGGGGGGCCGGCAAAGCCTTCCTCAACGGGGAGGCAGATAACGATACAGTTGTGGGAGCGGAAGGTAAGGACACCATGATAGAAAAATATGGAAACGATGTGTTCCAGTTCGCTGCAGGCATTGGCAGCGACCTGa  >  2:348809/1‑149 (MQ=255)
                                                                                                                                ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGaaa                                           >  5:389462/1‑102 (MQ=255)
                                                                                                                                 cTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAAc                                          <  6:389462/102‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
GGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGA  >  NC_002947/2921325‑2921595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑