Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	100.0%	161.9 / NA	46	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/27);  total (19/27)

AGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGC‑ATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGG  >  NC_002947/499059‑499331                                                                                                                                                 |                                                                                                                                   aGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGc                                                                                                                                 <  6:160449/148‑1 (MQ=255)  gACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCa                                                                                                                                >  4:289917/1‑148 (MQ=255)   aCCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt                                                                                                                               >  3:15982/1‑148 (MQ=255)   aCCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt                                                                                                                               >  3:179896/1‑148 (MQ=255)               cacaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑Cgg                                                                                                                  <  6:150205/148‑1 (MQ=255)               cacaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑Cgg                                                                                                                  <  4:380332/148‑1 (MQ=255)                 caAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGCA‑CCggc                                                                                                                >  4:48610/1‑148 (MQ=255)                 caAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGggc                                                                                                                <  4:179896/148‑1 (MQ=255)                      cACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGTCGGTGGTGAAGTACGCCATGGAGCACCAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGggcctggc                                                                                                           <  6:273465/148‑1 (MQ=255)                       aCGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGggcctggcct                                                                                                         >  2:6572/1‑149 (MQ=255)                       aCGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGggcctggccg                                                                                                         >  8:89953/1‑148 (MQ=255)                       aCGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATCCCCGCGAA‑CGggcctggcct                                                                                                         >  2:123044/1‑149 (MQ=255)                        cGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGgcctggcctg                                                                                                        <  2:155332/149‑1 (MQ=255)                           cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTTTGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCctgct                                                                                                      <  4:15982/148‑1 (MQ=255)                              aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATAGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGAt                                                                                                   <  2:244556/148‑1 (MQ=255)                                tcAGAAGTTGAAAGGGAACTCGCGGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGAtct                                                                                                 <  3:28406/147‑1 (MQ=255)                                gCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGAtctc                                                                                                >  7:365607/1‑149 (MQ=255)                                             gTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGcc                                                                                    >  5:209077/1‑148 (MQ=255)                                                aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAATCGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGc                                                                                 <  1:273498/149‑1 (MQ=255)                                                aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATctct                                                                                               >  7:10035/1‑134 (MQ=255)                                                aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATctct                                                                                               <  8:10035/134‑1 (MQ=255)                                                aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGcc                                                                                >  2:49113/1‑149 (MQ=255)                                                aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGc                                                                                 <  8:365607/148‑1 (MQ=255)                                                   tCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGc                                                                                          >  8:350052/1‑136 (MQ=255)                                                   tCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGc                                                                                          <  7:350052/136‑1 (MQ=255)                                                     gCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGAtctc                                                                                                >  4:321003/1‑128 (MQ=255)                                                      cTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATctct                                                                                               <  3:321003/128‑1 (MQ=255)                                                         gCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTcgcg                                                                        >  4:367799/1‑148 (MQ=255)                                                          cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                                       <  3:289917/148‑1 (MQ=255)                                                           ggAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGc                                                                     <  1:6572/149‑1 (MQ=255)                                                            gAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGc                                                                     <  4:210320/148‑1 (MQ=255)                                                            gAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTCCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGC‑a                                                                   >  7:356518/1‑149 (MQ=255)                                                               cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGC‑ATGc                                                                <  8:356518/149‑1 (MQ=255)                                                               cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCCCTATGGCTGAGCCGGCCTCTTCGCGGGC‑ATGc                                                                <  1:49113/149‑1 (MQ=255)                                                                tGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGC‑ATGc                                                                <  3:367799/148‑1 (MQ=255)                                                                 ggTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCgg                                                                                  >  6:142661/1‑130 (MQ=255)                                                                  gTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGc                                                                                 <  5:142661/130‑1 (MQ=255)                                                                           gCCTTGTAGCACAAGCTGATCTGTTTGCGACATAGGGGTGCCCTAAATTTGCGGAAGCCTATGTGGGAGCGGGCATTCCCGCGAA‑CGGGCCTGGCCTGGTGATCTCTATGGCTGTGCCGGCCTCTTCGGGGGC‑TTGCCCGCTCCcaca                                                     <  6:171905/148‑1 (MQ=255)                                                                            ccATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAAACCCGCGAA‑CGGGCCTGGCCAGCTGATGAAAATGGCTGAGCCGGCCTCTTCGCGGGC‑ATGCCCGCTGCcaca                                                     >  5:171905/1‑147 (MQ=255)                                                                                  aGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGC‑ATGCCCGCTCCCACAGGTTTCg                                              <  6:209077/148‑1 (MQ=255)                                                                                        aGCTGATCTGATTCCGACATACGGGGCCCCGAAACTTGCCGCAGCCTATGGGGGCGCGGGCAGGCCCGCGAC‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGC‑ATGCCCGCTCCCACAGGTTTCGTGGTTg                                        <  3:48610/148‑1 (MQ=38)                                                                                              tctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGC‑ATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGc                                  <  7:89953/148‑1 (MQ=255)                                                                                                            aCGGGTGCCCGAAACTTCCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGC‑ATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATc                   >  7:10305/1‑149 (MQ=255)                                                                                                              gggTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCgg                                                                                  >  5:230962/1‑85 (MQ=255)                                                                                                               ggTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGc                                                                                 <  6:230962/85‑1 (MQ=255)                                                                                                                              ccGAAGCCTATGTGGGAGCGGGCATCCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCTCTGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAggg  <  1:123044/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                   AGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAA‑CGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGC‑ATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGG  >  NC_002947/499059‑499331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑