| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 91.4% | 92.1 / ‑5.7 | 37 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (15/17); minor base G (0/3); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 100.0% | 107.7 / NA | 37 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (17/20); total (17/20) | |||||||||||
CGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTC > NC_002947/1499370‑1499634 || cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCcac < 1:299171/149‑1 (MQ=25)cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCca > 1:104421/1‑148 (MQ=25) aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGa < 8:185397/148‑1 (MQ=21) cGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCGGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTg < 7:208476/148‑1 (MQ=21) gCCATTCAGAG‑CTGAACGAAGCCGTGCCACCACAGCAGGACTCATGCTGCCAAACGGTGGGAGCGGGCGTGCGCGCGAAACAGGCG‑CGGC‑GGTGCATGGCACGGGCTTTGCCGGTGTTGGCGGGCA‑CGCCCGCTCCCACAGACTGAtt < 3:268930/148‑1 (MQ=21) cctttCAGTGCCTTAACTAAGCCTTGGCACCACCGCAGGACTGAGGCTGCCAAACGGTGGGAGGGGGGGTGCCCGCTAAACAGGCG‑CCGC‑GGTGCATGGCACGGGCTTTGCGGGGGTTGGCGGGCA‑GGCCCGCTCCCACAGACTGAtt < 4:347608/145‑1 (MQ=255) cATTCAGAGCCTGAACCACGCCTTGCCACCACAGCCGGACTGAGGCTGCCAAACGGGGGGAGCGGGCGGGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGc < 7:151249/149‑1 (MQ=18) ttCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCa < 2:264018/149‑1 (MQ=21) gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGGCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCGTTGGCGGTGTTAGCGGGCC‑CGCCCGCTGCCCCAGACGGATTGGCAtt > 1:362690/1‑147 (MQ=21) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCTAAACAGGCGCCCTCTGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGc < 1:331348/149‑1 (MQ=21) ttGGCACCACAGCAGGACTGAGGCGGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCGTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa < 5:364144/148‑1 (MQ=21) aCTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttt < 1:65983/149‑1 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTTCCCCCGAAACACGCG‑CCCC‑CGGGCAATGCACCCGCTTTGGCCGTGTTAGCGGGCACCC‑CCCCTCCCCCACACTGAATGGCCATAAGCGGGCCAAACGCGACACTGACGGCCCGgttttttt > 8:23313/1‑147 (MQ=255) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt < 2:104421/149‑1 (MQ=21) ggCTGCCAAAGGGTGGGTGCGGGCGTGCCGGCTAAACTGGCG‑CCGC‑GGTGCATGCAACCGGCTTTGCGGGTGTTCGCGGGAA‑GGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 8:341733/149‑1 (MQ=255) aCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACGGGCTTTGCCGGTGTTGGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc < 2:99801/147‑1 (MQ=25) cGGTGGGAGCGGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa < 2:272012/149‑1 (MQ=21) aGCGTGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc < 4:269136/148‑1 (MQ=25) gggCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt > 5:124815/1‑133 (MQ=21) gggCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATgc > 7:113308/1‑148 (MQ=25) gTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATCACTc > 4:376281/1‑148 (MQ=25) gCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGGATCATGACTCCCt > 2:199405/1‑149 (MQ=37) ccGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTccc > 4:390601/1‑146 (MQ=37) gcgAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa > 5:298230/1‑147 (MQ=37) cgAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCGGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa > 1:150170/1‑149 (MQ=37) cAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGACTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt < 5:324696/148‑1 (MQ=37) aGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa < 3:266835/148‑1 (MQ=37) aGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa < 3:390601/148‑1 (MQ=37) gcg‑cCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt > 5:9214/1‑147 (MQ=37) g‑ccgc‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 8:109183/1‑149 (MQ=37) gc‑gGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCaa > 3:151816/1‑145 (MQ=37) gCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCt > 4:35850/1‑77 (MQ=14) cATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa < 3:35850/77‑1 (MQ=14) ggcaccGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaaa > 3:283442/6‑148 (MQ=38) accGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATCACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGt > 5:168647/3‑148 (MQ=38) gCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGa < 7:109183/149‑1 (MQ=39) ccGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtc > 4:404375/1‑147 (MQ=255) ttcgcGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTccc > 3:125305/5‑102 (MQ=255) tcgcGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt < 4:125305/99‑1 (MQ=255) || CGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTC > NC_002947/1499370‑1499634 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |