| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 4,586,030 | 2 bp→TC | intergenic (+113/+101) | PP_4061 → / ← PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,586,030 | 0 | C | T | 100.0% | 135.5 / NA | 40 | intergenic (+113/+102) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/25); total (15/25) | |||||||||||
| * | NC_002947 | 4,586,031 | 0 | T | C | 95.0% | 127.8 / NA | 40 | intergenic (+114/+101) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (13/25); minor base A (2/0); total (15/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCA‑GGG‑A‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTG > NC_002947/4585892‑4586168 || gCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCTTTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gggtaa < 4:332491/148‑5 (MQ=255) ccAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gggtaaa > 6:380404/1‑143 (MQ=255) aGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGTTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGc < 3:153241/148‑1 (MQ=255) cACCGGTGAAGGCTTATGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTcc < 4:372448/148‑1 (MQ=255) cACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCt > 4:368315/1‑146 (MQ=255) aCCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTc < 3:368315/146‑1 (MQ=255) cGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAc > 7:335719/1‑148 (MQ=255) ggTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCa < 7:227076/149‑1 (MQ=255) tGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑gg > 1:230148/1‑149 (MQ=255) aGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑a < 5:380404/148‑1 (MQ=255) ggCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑A‑cc < 8:115060/149‑2 (MQ=255) gggTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATCTCCGGGTGCTTGGCTAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCgg < 1:59157/149‑1 (MQ=38) ggTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGc < 2:176916/149‑1 (MQ=39) gTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gg < 8:385972/116‑1 (MQ=255) gTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gg > 7:385972/1‑116 (MQ=255) aGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAC‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCt < 6:383279/148‑1 (MQ=38) gCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGAATgcgg < 2:230148/149‑1 (MQ=39) cACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGAGAAGGTGAAGATCTTGCATTGATCGGGCCCGCCTCTTCGCGGGTAAACCCGCTCATACCA‑GGG‑ACCCTGCAGGCCTTGAATgcggc > 2:156937/1‑149 (MQ=255) gCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGc < 8:337655/149‑1 (MQ=39) cGTGAACAGCTGATCTGCGCTACTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑gg > 4:43513/1‑99 (MQ=255) gTGAACAGCTGATCTGCGCTACTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGC‑ggg < 3:43513/99‑2 (MQ=255) gAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGTGATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCAATTAGCGGGTAAAACCCCTCCAAACAGGGG‑C‑CGGGCCGGCCGTGAATGGGGGGCGGcccc > 1:277725/1‑149 (MQ=255) cAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGGCC‑CGTGCCGGCCGTGAATTCGGCGGTGCCCCggg > 6:300217/1‑148 (MQ=37) cAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTgg < 4:101802/148‑1 (MQ=39) cAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGt < 1:156937/149‑1 (MQ=39) cAGCTGATCTGCGCTGCTCCATTGAAACGTTGAG‑GATGACCGGGTGCGTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGt < 2:108801/149‑1 (MQ=38) aGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGt < 6:100147/148‑1 (MQ=39) cTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGaa > 8:263607/1‑128 (MQ=38) cTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGaa < 7:263607/128‑1 (MQ=38) cTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAgg < 8:335719/149‑1 (MQ=39) cTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAgg < 2:75219/149‑1 (MQ=39) aTCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGaa < 2:283993/125‑1 (MQ=38) aTCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGaa > 1:283993/1‑125 (MQ=38) tCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCACTTAGCGGGTAAAACCCCAACAACCAGGGG‑C‑CCGGCCGGCCATGAATTCGGCGCGGGCCCGGGTAGGCGCGCGTTTTCCcg > 8:79076/1‑149 (MQ=255) ggTTGAG‑GATGACCGGGTGCTTGGCATGGTGAATATCTTGCATTGAGGGGGCCGGCCTCTTCGCGGGTAACCCCGCGCCTACCA‑GGG‑ACCGGGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGGGGGTTTACCCGCGAAGAggcc < 2:277725/149‑1 (MQ=37) ccGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg > 7:145915/1‑118 (MQ=37) ccGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg < 8:145915/118‑1 (MQ=37) gTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTgg > 4:325/1‑105 (MQ=37) tGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCA‑GGG‑ACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGt < 3:325/105‑1 (MQ=37) cggccTCTTCGCGGGTAAACCCGCTCCTAACAGGGG‑C‑CCGGGCGGCCTTGAATGCGGCGCTGCCCCGGGGAGGTGCGGGTTTACCCGCGGATCGGCCGGCCGCGCCCGGCCAACACCCCGCCGATGCAGCCGCAATCTGCGCCCCCCTg > 2:7588/1‑149 (MQ=255) || GCCAGGAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAG‑GATGACCGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTCTGC‑GGTAAACCCGCTCCTACCA‑GGG‑A‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCCGCGCCAGCACATCAACCCGCAGATGCAGCCGCAATCTCCGCCACACTG > NC_002947/4585892‑4586168 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |