| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 97.7% | 124.3 / NA | 43 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (17/25); total (18/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 100.0% | 126.0 / NA | 43 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (18/25); total (18/25) | |||||||||||
GCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAG > NC_002947/1499337‑1499611 || gCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGGCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGc > 7:185397/1‑149 (MQ=255) aCGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTg < 1:252080/149‑1 (MQ=255) aCGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTg > 2:299171/1‑149 (MQ=255) cGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGc < 7:307933/149‑1 (MQ=255) gggCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGc < 3:178462/148‑1 (MQ=255) gCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCgg > 2:65983/1‑149 (MQ=255) cGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGgt < 3:373011/148‑1 (MQ=255) cGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGgt < 3:102510/148‑1 (MQ=255) ttCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGCGGGCCCCCGAAAAAGGGC‑CCCC‑GGGGCATGCACCCGCGTTTGCCgtgttacc > 7:341733/1‑142 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGttcgcgg < 2:377273/148‑6 (MQ=255) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCcac < 1:299171/149‑1 (MQ=25) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCca > 1:104421/1‑148 (MQ=25) aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGa < 8:185397/148‑1 (MQ=21) cGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCGGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTg < 7:208476/148‑1 (MQ=21) gCCATTCAGAG‑CTGAACGAAGCCGTGCCACCACAGCAGGACTCATGCTGCCAAACGGTGGGAGC‑GGGCGTGCGCGCGAAACAGGCG‑CGGC‑GGTGCATGGCACGGGCTTTGCCGGTGTTGGCGGGCA‑CGCCCGCTCCCACAGACTGAtt < 3:268930/148‑1 (MQ=21) cctttCAGTGCCTTAACTAAGCCTTGGCACCACCGCAGGACTGAGGCTGCCAAACGGTGGGAGG‑GGGGGTGCCCGCTAAACAGGCG‑CCGC‑GGTGCATGGCACGGGCTTTGCGGGGGTTGGCGGGCA‑GGCCCGCTCCCACAGACTGAtt < 4:347608/145‑1 (MQ=255) cATTCAGAGCCTGAACCACGCCTTGCCACCACAGCCGGACTGAGGCTGCCAAACGGGGGGAGC‑GGGCGGGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGc < 7:151249/149‑1 (MQ=18) ttCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCa < 2:264018/149‑1 (MQ=21) gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGGCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCGTTGGCGGTGTTAGCGGGCC‑CGCCCGCTGCCCCAGACGGATTGGCAtt > 1:362690/1‑147 (MQ=21) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCTAAACAGGCGCCCTCTGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGc < 1:331348/149‑1 (MQ=21) ttGGCACCACAGCAGGACTGAGGCGGCCAAACGGGGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCGTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa < 5:364144/148‑1 (MQ=21) aCTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttt < 1:65983/149‑1 (MQ=21) aGGCTGCCAAACGGTGGGAGC‑GGGCGTTCCCCCGAAACACGCG‑CCCC‑CGGGCAATGCACCCGCTTTGGCCGTGTTAGCGGGCACCC‑CCCCTCCCCCACACTGAATGGCCATAAGCGGGCCAAACGCGACACTGACGGCCCGgttttttt > 8:23313/1‑147 (MQ=255) aGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt < 2:104421/149‑1 (MQ=21) ggCTGCCAAAGGGTGGGTGC‑GGGCGTGCCGGCTAAACTGGCG‑CCGC‑GGTGCATGCAACCGGCTTTGCGGGTGTTCGCGGGAA‑GGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 8:341733/149‑1 (MQ=255) aCGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACGGGCTTTGCCGGTGTTGGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc < 2:99801/147‑1 (MQ=25) cGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa < 2:272012/149‑1 (MQ=21) aGC‑GTGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc < 4:269136/148‑1 (MQ=25) gggCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt > 5:124815/1‑133 (MQ=21) gggCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATgc > 7:113308/1‑148 (MQ=25) gTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATCACTc > 4:376281/1‑148 (MQ=25) gCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGGATCATGACTCCCt > 2:199405/1‑149 (MQ=37) ccGCGAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTccc > 4:390601/1‑146 (MQ=37) gcgAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa > 5:298230/1‑147 (MQ=37) cgAAACAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCGGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa > 1:150170/1‑149 (MQ=37) cAGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGACTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCt < 5:324696/148‑1 (MQ=37) aGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa < 3:266835/148‑1 (MQ=37) aGGCG‑CCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTa < 3:390601/148‑1 (MQ=37) gcg‑cCGC‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt > 5:9214/1‑147 (MQ=37) g‑ccgc‑GGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 8:109183/1‑149 (MQ=37) gc‑gGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCaa > 3:151816/1‑145 (MQ=37) gCATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCt > 4:35850/1‑77 (MQ=14) cATGGCACCGGCTTTGCCGGTGTTCGCGGGCA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa < 3:35850/77‑1 (MQ=14) || GCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCG‑CCGC‑GGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAG > NC_002947/1499337‑1499611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |