| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,508 | +C | intergenic (+85/‑79) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,506 | 1 | . | C | 97.3% | 103.2 / ‑2.9 | 37 | intergenic (+83/‑81) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (15/21); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01 | |||||||||||
GCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTGTT‑TCGGG‑CACG‑CCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAC > NC_002947/1499361‑1499643 | gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTTCCCCCGCAAAAGGCGCCCCCGTGCATTGCACC‑CGCTATTCCGGTGTTACCGGG‑CAcc‑cc > 6:134239/1‑149 (MQ=255) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAAAAAGCGCCCCCGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CCCG‑cccccccccc > 4:336869/1‑146 (MQ=21) gCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACt > 7:276278/1‑148 (MQ=21) ggCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑At > 5:267629/1‑149 (MQ=21) gCCTTTCAGAGCCTGAACGAAGCGTTGGCACCAACGCAGGACTGAGGCTGCCAAACGGGGGGCGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGC‑TGGGCACGGGCTTTGCGGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑Att < 8:221099/149‑1 (MQ=21) gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑Att < 8:276278/149‑1 (MQ=21) cATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGc < 3:84971/149‑1 (MQ=21) agagCCTCAAGTCAGCCTTGCCACCACTCCCGTAGTGAGGCTCCAAAAGGGTGGCTGCGGGCGGGCCCGCTAAACTGGCGCCGGGGTGATTGCCCCC‑GGCTTTGCGGGTGTTCGGGGG‑CAGGCCCGCTCCCACAGACTG‑ATTGCCAtt < 8:17180/148‑1 (MQ=255) ccTGACCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCa < 7:143567/142‑1 (MQ=21) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCAtt > 6:265611/1‑144 (MQ=21) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCAtt < 5:265611/144‑1 (MQ=21) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCg < 5:207128/149‑1 (MQ=21) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCCTGGCCCCGAAACACGGGCCGCGGTGCAATGCACC‑CGCGTTGGCGGTTTTAGCGGG‑CACGCCCGCACCCACACCCAGAATTGCCA‑TAA‑GCggg > 6:322620/1‑149 (MQ=255) cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTAGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCg > 2:267452/1‑149 (MQ=25) gACTGAGGCTGCCAAACGGTGCGAGCGGGCGTGCCCGCGAAACAAGCGCCCCGGTGCATGGCACC‑GGCTTTGCCCCTGTTGGCGGGCCCCC‑CCGCGCCCAAAGACAG‑AATTCCATTAT‑ACCGCCCAATAGCTTCACTGACGGCCCGgtt > 8:203971/1‑149 (MQ=9) tggCTGCCATACGTGGGTAGGGGGCGTGCCCGCGAAACATGCGCGGCGGTGCTTGGCACG‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 7:203971/146‑1 (MQ=21) gCTGCCAAACGGTGGCTGCGGGCGTGCCCGCGAAACAGGCGCGGCGGTGCATGGCACC‑GGCTTTGCCGGTTTTCGCGGT‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg < 6:279518/149‑1 (MQ=21) cGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa < 3:336869/149‑1 (MQ=21) aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 6:267629/149‑1 (MQ=25) gggggCGTGCCGGGGAAACTGGCGCGGCGGTGCTTGGCAGC‑GGCTTTGCGGGTGTTCGCGGT‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAATGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc < 1:97227/146‑1 (MQ=255) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACt > 8:161259/1‑149 (MQ=25) cccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 1:181507/1‑148 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 2:321829/1‑147 (MQ=37) cgccgcGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCaa < 1:321829/149‑1 (MQ=37) gccgcGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg < 1:267452/149‑1 (MQ=37) cc‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTcc > 1:146356/2‑149 (MQ=39) ggCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGcc > 1:345355/1‑60 (MQ=14) ggCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGcc < 2:345355/60‑1 (MQ=14) gCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc > 4:35079/1‑131 (MQ=38) gCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc < 3:35079/131‑1 (MQ=38) gCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGa > 7:192728/1‑149 (MQ=39) tttGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:110545/104‑1 (MQ=37) tttGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa > 2:110545/1‑104 (MQ=37) ccGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtct < 5:134239/148‑1 (MQ=255) cgcGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAc < 7:161259/146‑1 (MQ=255) gcGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTcc > 6:351441/2‑131 (MQ=255) gcGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTcc < 5:351441/130‑1 (MQ=255) gcGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCACGACTTCTTCCATg < 5:373746/144‑1 (MQ=255) gcGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCATAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATg > 6:373746/2‑145 (MQ=255) | GCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTGTT‑TCGGG‑CACG‑CCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAC > NC_002947/1499361‑1499643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |