| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 96.4% | 86.1 / ‑2.8 | 28 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (16/11); total (16/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
TGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTT > NC_002947/1932491‑1932772 | tGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑c < 6:23873/149‑1 (MQ=255) ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGCAggg > 4:150227/1‑149 (MQ=255) tCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAAtt > 2:50857/1‑149 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc > 4:86896/1‑149 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCGCCTACAAAGGATATGCGTCCCCGGTAGGCGCGGGGTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc > 3:252869/1‑149 (MQ=38) cgcgaagggCCGCAAAGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGtt > 6:127561/7‑148 (MQ=37) tGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCTACAAAAAAAGATATGCGTGCCCAGTAGGAGCGGGCTT‑GCCCGCGAAGGGCAGCAAGGCCGCCCCAAAATGTCGACAAAGCGGATCAGCGCTCCGGCAGGGTGATGTTAAGc > 2:302252/1‑148 (MQ=25) ttttGCGGCCGTCCCGGGGCACCCCCGCTCAAAAAAAGCATCTGCTCCCCGTGTTGCTGGGGGCTTCC‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑Tc < 1:177949/148‑1 (MQ=255) cTTCGCGGCCACGCCCGCTCCTACAACCTATAGGCGTCCCCTTTAGGAGCGGGTTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGc < 3:33849/149‑1 (MQ=255) gcgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTg < 5:127561/149‑1 (MQ=255) cAAGCCCGCTCCTACAAAGTATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGtttt < 5:100525/149‑1 (MQ=255) aaGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTc > 5:314214/1‑149 (MQ=255) aGCCCGCTCCTACAATGTTTAGGCGTCCCTTGTAGGTGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTcc < 8:144162/149‑1 (MQ=38) ccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTCCAGc > 6:191681/1‑149 (MQ=255) cGCTCATAAAAAGTATAGGCGTCCCCTGTAGTAGGGGGCTG‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAACTCGAGCAGCTGCCCTGGTTTTCCAGc < 1:193642/149‑1 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGtttt > 2:268458/1‑138 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGtttt < 1:268458/138‑1 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTCCAGCTCGat < 6:314214/148‑1 (MQ=255) cAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGc < 1:302252/149‑1 (MQ=255) aaGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca > 2:138463/1‑148 (MQ=255) tatGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTc < 5:249333/129‑1 (MQ=255) tatGCGTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTc > 6:249333/1‑129 (MQ=255) gTCCCCTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg > 3:89778/1‑149 (MQ=255) cccTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGcc > 5:50419/1‑148 (MQ=255) ccTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt < 5:254711/144‑1 (MQ=255) ccTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt > 6:254711/1‑144 (MQ=255) ccTGTAGGAGCGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGa > 5:233139/1‑149 (MQ=255) gCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGtt < 5:97115/140‑1 (MQ=255) gCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGtt > 6:97115/1‑140 (MQ=255) | TGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAA‑TCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTT > NC_002947/1932491‑1932772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |