| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 3,845,705 | +G | intergenic (‑72/+26) | PP_3394 ← / ← PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,845,703 | 1 | . | G | 94.3% | 108.7 / 0.6 | 35 | intergenic (‑70/+28) | PP_3394/PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (20/13); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.50e-01 | |||||||||||
GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGC > NC_002947/3845557‑3845842 | gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑Tc‑gc‑g < 5:147913/148‑1 (MQ=255)gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑Tc‑gc‑g < 3:354308/148‑1 (MQ=255) ctgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GC‑ggg < 6:147469/149‑2 (MQ=255) ctgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATCGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GC‑ggg < 6:36269/149‑2 (MQ=255) tgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GC‑gggc < 1:331328/149‑3 (MQ=255) ggCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATcc < 1:173923/148‑1 (MQ=255) ggCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCa < 6:92274/149‑1 (MQ=255) aTCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGt > 8:250149/1‑147 (MQ=255) tCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAg > 6:218939/1‑149 (MQ=255) ctctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGAt < 6:257597/149‑1 (MQ=255) tctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGAt > 7:94571/1‑148 (MQ=255) tGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATAGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAg > 2:325575/1‑149 (MQ=255) cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGcc > 3:277646/1‑149 (MQ=255) cATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCCGCAGCCACGATGGTGCAGCCCgcgc > 2:339257/1‑149 (MQ=255) tCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGccc > 5:363081/1‑149 (MQ=255) gCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGc > 8:39667/1‑149 (MQ=255) attCATTGATTGTTTTTTTTGTCATCGCGGTCTCTGTGTCTTAAGCGTTGCGCCAGACTAATTGCTGGATGAGGGGCTGAAATGACGTGTT‑TC‑GCGGGCTGATCCATCTTCAGCCAGGATGGTTCAGCCCGCGCCCTGCCAGGCCGGt < 6:363081/147‑1 (MQ=255) tattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAg > 3:99749/1‑149 (MQ=255) ttgtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtgga > 7:91784/1‑148 (MQ=255) aTCGGGGTCTGTGTGTCTCAAGGGTTGCGCACTAATAATTGCTGTTTGAGGGGACTAAAAGACGTGTTGGG‑GG‑GGGTCACCAAGCGTCAGCCACGTTGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGc < 4:277646/149‑1 (MQ=255) aTCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGc < 5:382162/149‑1 (MQ=255) tctctGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccga > 7:164673/1‑149 (MQ=255) tgtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagc < 2:116021/147‑1 (MQ=255) gAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCAATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGc > 2:116796/1‑149 (MQ=255) aaGCGTTGCGCCAGACTATGAGCTGGATGACGGGCATAAAAGAGGTGTT‑TCGGG‑GGCTGATCCATCGTCAGCCACTATGTATCAGCCCGCGCCCt < 2:228639/95‑1 (MQ=255) aaGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCt > 1:228639/1‑95 (MQ=255) aaGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAg > 7:292710/1‑132 (MQ=255) aaGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAg < 8:292710/132‑1 (MQ=255) aaGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATAAGCCCCCGCCCt > 7:123193/1‑95 (MQ=255) cGTGGCGCCAGACTAAGAGCTGGATGTCGGGCATAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCgaga < 6:293012/149‑1 (MQ=255) ccAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGtgtg > 3:158037/1‑149 (MQ=255) cAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGc > 3:251884/1‑149 (MQ=255) ggATGACGGGCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATg > 3:238846/1‑148 (MQ=255) cGGGCATAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGa < 7:250149/149‑1 (MQ=255) gggCAGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGAc > 8:237324/1‑149 (MQ=255) aGAAAAGACGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAAt > 7:25146/1‑149 (MQ=255) aCGTGTT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGATTTCACCaa < 8:94571/149‑1 (MQ=255) gtgtT‑TC‑GCGGGCTTATCCATCGTCTCCAACTATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTTGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAgc < 1:330841/149‑1 (MQ=255) gtgtT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAgc < 4:251884/149‑1 (MQ=255) gtgtT‑TC‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAgc < 1:325575/149‑1 (MQ=255) | GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTT‑TC‑GC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGC > NC_002947/3845557‑3845842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |