Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,419,549	+C	intergenic (‑38/+98)	PP_1242 ← / ← PP_1244	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,419,548	1	.	C	100.0%	158.4 / NA	47	intergenic (‑37/+99)	PP_1242/PP_1244	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (24/23);  total (24/23)

CTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGG‑CTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATGCGAC  >  NC_002947/1419401‑1419683                                                                                                                                                      |                                                                                                                                        cTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                                        <  5:139997/149‑1 (MQ=255) cTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                                        <  6:132956/149‑1 (MQ=255)  tGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGggctgg‑cc                                                                                                                                       >  4:332406/1‑148 (MQ=255)          gATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTcgcg                                                                                                                                >  2:35335/1‑149 (MQ=255)            tGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCggg                                                                                                                              <  1:35335/149‑1 (MQ=255)                      gATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCt                                                                                                                    >  6:292849/1‑149 (MQ=255)                         gCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGc                                                                                                                         >  3:207928/1‑141 (MQ=255)                         gCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGc                                                                                                                         <  4:207928/141‑1 (MQ=255)                          cGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCca                                                                                                                <  4:46064/149‑1 (MQ=255)                           ggAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCca                                                                                                                >  5:11928/1‑148 (MQ=255)                            gAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCcaca                                                                                                              <  3:332406/149‑1 (MQ=255)                             aaCAGCGGGTGAGTGTTGTTTTCGCCTTGTTAGATGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAg                                                                                                             <  8:166646/149‑1 (MQ=255)                                  cGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACt                                                                                                        >  4:215545/1‑149 (MQ=255)                                   gggTGCGTGTTGTTTTCGCCTTGGTCGAAGCAGGTCTTGCCGTCTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGTACGCCCGCTCCCACAGGTACtg                                                                                                       <  2:335160/149‑1 (MQ=39)                                           gttgttTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGc                                                                                               >  5:385137/1‑149 (MQ=255)                                              gttTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGt                                                                                             >  6:232298/1‑148 (MQ=255)                                               ttttCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTg                                                                                            <  3:215545/148‑1 (MQ=255)                                                     ccTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtg                                                                                     >  4:355251/1‑149 (MQ=255)                                                         ggTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtgtg                                                                                   <  5:232298/147‑1 (MQ=255)                                                         ggTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtgtg                                                                                   <  2:77562/147‑1 (MQ=255)                                                         ggTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTgtgg                                                                                  <  3:166089/148‑1 (MQ=255)                                                         ggTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTgtgg                                                                                  >  4:166089/1‑148 (MQ=255)                                                          gTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTgtggt                                                                                 >  2:30053/1‑148 (MQ=255)                                                            aGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGAc                                                                              >  7:8819/1‑149 (MQ=255)                                                                gCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCCCAGGTTCTGTGGAGGGCGTGTCGGCTGTGGTTAcccgg                                                                          >  3:166591/1‑147 (MQ=255)                                                                gCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTACCCCAGGTAATGTGGAGTGCGTGACGGCTGTGGTGAcccgg                                                                          >  3:172294/1‑147 (MQ=255)                                                                  aGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCtgtg                                                                        <  3:355251/149‑1 (MQ=255)                                                                          ccGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTa                                                                                                          <  5:125723/107‑1 (MQ=255)                                                                          ccGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTa                                                                                                          >  6:125723/1‑107 (MQ=255)                                                                           cGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGc                                                               <  6:11928/149‑1 (MQ=255)                                                                                 tggtggGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGc                                                           <  3:113045/147‑1 (MQ=255)                                                                                    tggGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCcgcg                                                      <  1:30053/149‑1 (MQ=255)                                                                                    tggGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCCCTACCACAGGTTATTTGGAGTTCGTTACGGGTGTGGTTTCCCGGTGGGTGCGGGCGTGCCcgc                                                       >  5:209399/1‑148 (MQ=255)                                                                                      ggTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTa                                                                                                          <  5:114187/95‑1 (MQ=255)                                                                                      ggTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTCCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTa                                                                                                          >  6:114187/1‑95 (MQ=255)                                                                                      ggTTCTGTGGTGTTGTCGGTGGGCTTTGTTTTGTTTCCC‑TGGATTGTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGaa                                                    <  8:8819/149‑1 (MQ=37)                                                                                           tgttgtgttgTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCAACCCCCCAACCCCAGGGAATGTTGAGTGGGTGACGGGTGTGGTGACCCGGTGGGGGCGGGCGTGGCCGCGAATAggg                                               >  7:38863/1‑148 (MQ=255)                                                                                           tgttgtgttgTCGGGGGGCTTTGTTTTTTTCCCC‑TGGATTCTGGGTTGCCTGGGCGGGCCTCTTCGCGGCCACGCCCGCTCCCACCGGTAGTGTGCCGTGCGTGACGGCTGTGGTCACCCTGTGGGCGGGGGCGTGCCCGCGAAGAGGc                                               <  6:209399/149‑1 (MQ=255)                                                                                                tgttgtCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGGAGTTCGTGAAGGGTGTGGTGACCCTGTGGGAGCGGGCGTGGCCGCGAAGAGGCCTGAc                                          >  7:340612/1‑149 (MQ=37)                                                                                                tgttgtCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTAGCGGGCAAGCCCCCTCCCACAGGTACTGTGGAGTGCGTGACGGCTGTTGTGACCCTGGGGGGGCGGGCGTGCCCGCGAAGAGGCCTGAc                                          >  5:10006/1‑149 (MQ=255)                                                                                                    gtCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCcgc                                                       >  4:348797/1‑132 (MQ=255)                                                                                                    gtCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCcgc                                                       <  3:348797/132‑1 (MQ=255)                                                                                                     tCGGGGGGCATTGTTTTGTTGCCG‑TGGTTTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACATGTACTGTGCAGTGCGTGACGGCTTTGGTTACCGTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGc                                      <  6:385137/148‑1 (MQ=25)                                                                                                           ggCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGc                                <  5:292849/148‑1 (MQ=255)                                                                                                                 gttttttttCCCGTGTTTTGTGGGGGGCGTGGGCGGGCCTTTGCGCGGCCCCGCCGGCCCCCACTGTTCCTGTGCTGTGCTTCACGGGGGGGGTCACCTTGGGGTTGGGGGGGGGCCCGCTAAGAGGCCTGACCTGCTAATGCAAGgt                           <  4:172294/148‑1 (MQ=255)                                                                                                                  ttttgtttCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAATAGGCCTGACCTGCTAAt                                  >  3:221557/1‑139 (MQ=255)                                                                                                                   tttgtttCCC‑TGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCCCAGTTAAAGTGCCGTGCGAGCCGGCTGGCGGTACCCGGTGGGAGCGGGCGGGCCCGCGAAAAGGCCTGCCCTGCTTATGCaacgggg                         >  6:277197/1‑142 (MQ=255)                                                                                                                               ggATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTggg            >  8:292941/1‑149 (MQ=255)                                                                                                                                      gggTTGCCTGGGCTGGCCTCTTAGCGGGCCCCCCCCCACCCCCACGTACATTGGAATGCGGGACGGCAGTGGTGACCCCGTGGGAGCGGGCGTGCCCGCGAACAGGCCTGACCAGCGAAATCAAAGTGTTAGGCACTGTGGGTTAAtta     >  8:324463/1‑147 (MQ=255)                                                                                                                                         ttGCCGGGGCTGGCCTCTTCGCGGCCACGCCCGCTCCCACTGGAACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGGGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATGCGAc  <  7:324463/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                        CTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCC‑TGGATTCTGGGTTGCCTGGGCTGG‑CTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATGCGAC  >  NC_002947/1419401‑1419683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑