Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_002947	5,681,415	+CGGG	intergenic (‑133/+71)	PP_4986 ← / ← PP_4987	hemolysin III family channel protein/putative Chemotaxis protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 5681415	4 (0.060)	34 (0.550) +CGGG	27/270	2.1	89.8%	intergenic (‑133/+71)	PP_4986/PP_4987	hemolysin III family channel protein/putative Chemotaxis protein
	?	NC_002947	5681416 =	4 (0.060)					intergenic (‑134/+70)	PP_4986/PP_4987	hemolysin III family channel protein/putative Chemotaxis protein

GCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCA  >  NC_002947/5681283‑5681559                                                                                                                                     |                                                                                                                                                     gCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcg                                                                                                                                                    <  4:156062‑M1/135‑3 (MQ=255) gCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcg                                                                                                                                                    >  3:156062‑M1/1‑133 (MQ=255)   aaaCACAGACCTCCTGAGTTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagcc                                                                                                                                    <  6:314351‑M1/149‑19 (MQ=255)   aaaCACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagcc                                                                                                                                    <  1:162849‑M1/149‑19 (MQ=255)       acaGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgccgcccgat                                                                                                                                >  8:55639‑M1/1‑127 (MQ=255)                ctGAGGTGGGCTGGGTTCCTGTTGGCATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggccgctgcgcagccctatcgcgaca                                                                                                                         <  7:55639‑M1/147‑30 (MQ=255)                          cTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcc                                                                                                             >  1:30371‑M1/1‑108 (MQ=255)                                         gATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgaca                                                                                                                         <  3:235794‑M1/122‑30 (MQ=255)                                         gATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgaca                                                                                                                         >  4:235794‑M1/1‑93 (MQ=255)                                            gCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcag                                                                                                                                      <  7:95699‑M1/106‑17 (MQ=255)                                            gCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcag                                                                                                                                      >  8:95699‑M1/1‑90 (MQ=255)                                                         ttCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggcagctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcac                                                                               >  2:233961‑M1/1‑77 (MQ=255)                                                         ttCTTTGTGGGAGCGGCCATGTGTCGCGAAAGGGGCGCCAAGCGGCCCCACGCTTTCATTGCCACAGCAAGAACCGCCAGGGCAGCTGCGCAGCCCTATCGCGCCACAAGGCCGCTCCCACACCACACCACCCGGCCCTGCGCCacac                                                                               >  8:252733/1‑148 (MQ=18)                                                           cTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcg                                                                                                                                                    <  8:109109‑M1/77‑3 (MQ=255)                                                           cTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcg                                                                                                                                                    >  7:109109‑M1/1‑75 (MQ=255)                                                            tttGTGGGAGCGGCCTTGTGTCGCGAAACGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCCGGGCTGCTGCGCCGCCCGATAGCGCCACAACGCCCCGCCCCCGCCCCCCCCCCCCGCCCAGCGCCGCACATc                                                                            >  2:224231/1‑148 (MQ=12)                                                              tgtgGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatc                                                                            <  5:373987‑M1/146‑75 (MQ=255)                                                              tgggggagcGGCCTTGTGTCGCGAACGGGGCGCTAAGCGGCCCCAGGCTTTCATTGCCACTGCATGACCCGCcggggcggctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatc                                                                            <  4:275972‑M1/143‑75 (MQ=255)                                                                tgGGAGCGGCCTTGTGGCGCTAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGACCCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaac                                                                       <  7:252733‑M1/149‑80 (MQ=255)                                                                        gCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggt                                                                 >  5:166598‑M1/1‑62 (MQ=255)                                                                              tggcgctaatGGGGGGCTAAGGGCCCCCAGGTTTACATTGCCAATGCATGAACCGCcggggcggctgcgcagccctatcgccacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttg                                                          <  8:61385‑M1/145‑93 (MQ=255)                                                                                         gggCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGGTGCGCAGCCCTATAGCCACACAAGGCCCCTCCCACTCCAGACCACCAGCCCCATCCCCGAACATACAAACCAAGGACAGGATGAACATCAGCGt                                               >  2:216029/1‑148 (MQ=255)                                                                                           gcgcGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcccctactaaccaaggtcaggttgcagatccgcgtcac                                            >  4:75131‑M1/1‑43 (MQ=255)                                                                                           gcgcGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgc                                                                                      <  8:94908‑M1/107‑65 (MQ=255)                                                                                           gcgcGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgc                                                                                      >  7:94908‑M1/1‑43 (MQ=255)                                                                                                   cGGCCCCAGGCTTACATTGCCACTGCATCACCCGCcgggggtgcggcgcagccctagcgctaaacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagc                                    <  1:224231‑M1/149‑115 (MQ=255)                                                                                                       cccAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagccttt                                >  5:121934‑M1/1‑31 (MQ=255)                                                                                                        ccAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttc                               <  4:241485‑M1/149‑120 (MQ=255)                                                                                                              tttCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatagcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgc                         >  7:188541‑M1/1‑24 (MQ=255)                                                                                                                 cATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaat                           <  4:356291‑M1/144‑124 (MQ=255)                                                                                                                 cATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaat                           >  3:356291‑M1/1‑21 (MQ=255)                                                                                                                  aTTGCCACTGCATGAACCGCCGGGGGTGGTGGGCCGCCCTATAGCGGCCCAAGGGCGCGGCCACGGCCGACCCCCCGGCCCCGCGCCGCACCGCCTAACCCAGGGGAGGTTGGAGATCCGCGGGCCGGCCCAGCCGTTGCAATGCGGcc                     >  6:247173/1‑149 (MQ=255)                                                                                                                   ttGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagccccccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgacca                    >  8:270833‑M1/1‑19 (MQ=255)                                                                                                                   ttGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatccaaaccaaggtcagggtgcagctccgcgtcacgcaccagccgttccaatgcgcc                      >  5:21286‑M1/1‑19 (MQ=255)                                                                                                                         cTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggtccg              <  6:166598‑M1/149‑137 (MQ=255)                                                                                                                         cTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggtccg              <  6:121934‑M1/149‑137 (MQ=255)                                                                                                                          tGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggtccgg             >  5:115949‑M1/1‑12 (MQ=255)                                                                                                                                aaCCGCcggggcggctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggtccggtaccc        <  1:233961‑M1/148‑143 (MQ=255)                                                                                                                                 aCCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggtccggtacccgc      >  5:145351‑M1/1‑5 (MQ=255)                                                                                                                                     ccggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggtccggtacccgcgcca  >  8:55006‑M1/1‑1 (MQ=255)                                                                                                                                     ccggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggtccggtacccgc      <  2:326456‑M1/145‑145 (MQ=255)                                                                                                                                     ccggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggtccggtacccgc      >  1:326456‑M1/1‑1 (MQ=255)                                                                                                                                     |                                                                                                                                                     GCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑GCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCA  >  NC_002947/5681283‑5681559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.