Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,986,8410TA74.0% 85.3 / 27.4 50intergenic (+121/‑78)PP_5245/kefB‑IIIAraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base T (6/7);  new base A (18/19);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCG  >  NC_002947/5986707‑5986982
                                                                                                                                      |                                                                                                                                             
aaCAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTa                                                                                                                                 >  8:212906/1‑149 (MQ=255)
   aGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTAccc                                                                                                                              <  4:46500/149‑1 (MQ=255)
   aGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTAccc                                                                                                                              >  3:217453/1‑149 (MQ=255)
     tCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCt                                                                                                                            >  4:258613/1‑149 (MQ=255)
     tCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTAAGATGATTGAATGACGACACACTAcccc                                                                                                                             >  4:211479/1‑148 (MQ=255)
          ttAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGaa                                                                                                                       <  8:207825/149‑1 (MQ=255)
                   ccTTCTTCGCGGGGGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCg                                                                                                                <  4:158475/147‑1 (MQ=255)
                     ttcttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTg                                                                                                                                            <  5:27329/117‑1 (MQ=255)
                     ttcttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTg                                                                                                                                            >  6:27329/1‑117 (MQ=255)
                       cttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGt                                                                                                                   <  5:82008/140‑1 (MQ=255)
                       cttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGt                                                                                                                   >  6:82008/1‑140 (MQ=255)
                       ctgcgcgGTCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCaaa                                                                                                           <  3:211479/145‑1 (MQ=255)
                             gggCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTc                                                                                                     >  8:278144/1‑148 (MQ=255)
                              ggCGCGCCCGCTCCCACAGATATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCAACTATTGTCACTCTGGACAGTACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGa                                                                                                   <  7:67968/149‑1 (MQ=255)
                               gcgcgcCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGaa                                                                                                  <  7:278144/149‑1 (MQ=255)
                                cgcgcCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAAc                                                                                                 <  4:217453/149‑1 (MQ=255)
                                             acaGAGATAGCACACGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGACCGTCAAACTGTTCGAACGCTGCCCCGGCAg                                                                                    >  2:88595/1‑149 (MQ=255)
                                                gagaTAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTc                                                                                 <  7:212906/149‑1 (MQ=255)
                                                   aTAGCACATGCATATAGCCCAGGTAAGCGCCGTCACCGAATGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATg                                                                              <  8:315734/149‑1 (MQ=255)
                                                      gCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGa                                                                            <  4:138110/148‑1 (MQ=255)
                                                          aGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCaaa                                                                                                           >  4:352741/1‑113 (MQ=255)
                                                              cTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGGCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGcc                                                                   <  1:88595/149‑1 (MQ=255)
                                                                 gAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCaa                                                                 <  3:258613/148‑1 (MQ=255)
                                                                   gCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGc                                                              >  4:62393/1‑149 (MQ=255)
                                                                    cccAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCAGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCg                                                                                        >  2:315431/1‑122 (MQ=255)
                                                                     ccAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCca                                                            >  3:316548/1‑149 (MQ=255)
                                                                       aGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCcaca                                                          >  8:292699/1‑149 (MQ=255)
                                                                               gcCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCa                                                  <  2:107195/149‑1 (MQ=255)
                                                                                   gCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCt                                              >  7:321673/1‑149 (MQ=255)
                                                                                        gATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTg                                          >  7:314840/1‑148 (MQ=255)
                                                                                            gTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCaaa                                      <  7:292699/148‑1 (MQ=255)
                                                                                             tCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAAcc                                    <  8:321673/149‑1 (MQ=255)
                                                                                                   tGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATgg                                                                             >  1:365570/1‑102 (MQ=255)
                                                                                                   tGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATgg                                                                             <  2:365570/102‑1 (MQ=255)
                                                                                                     gacaggacaATTTTTCTTCCCGTCATATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTCCTGCAAACCGCgg                                <  7:344191/145‑1 (MQ=255)
                                                                                                     gacaggacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGa                             >  3:276147/1‑148 (MQ=255)
                                                                                                         ggacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTc                        <  4:276147/149‑1 (MQ=255)
                                                                                                          gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGc                                                              >  4:296883/1‑110 (MQ=255)
                                                                                                          gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGc                                                              <  3:296883/110‑1 (MQ=255)
                                                                                                          gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCAAACTACCCCTCACAATAGACCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTAATGGAGTACGAGCCAATGCAACACGAAGGCAGACTGCTGCAAACAGCTGTGATATTca                       >  7:174417/1‑148 (MQ=255)
                                                                                                          gacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGt                                                                          <  1:251470/98‑1 (MQ=255)
                                                                                                          gacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGt                                                                          >  2:251470/1‑98 (MQ=255)
                                                                                                          gacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTcc                       >  5:135425/1‑149 (MQ=255)
                                                                                                          gacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTcc                       >  8:54520/1‑149 (MQ=255)
                                                                                                             aaTTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGc                    <  3:62393/149‑1 (MQ=255)
                                                                                                                       cccGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTgccgcc            <  4:316548/147‑1 (MQ=255)
                                                                                                                         cGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTc         >  4:142738/1‑148 (MQ=255)
                                                                                                                          gTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCt       >  7:300120/1‑149 (MQ=255)
                                                                                                                             aGATTATTTTCTGACGCCACACTTCCCCTCAGAATTGTCCGTCAATCTGTTCGAACGCTGCCCCGGTAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCAGTCCTCg     <  8:174417/148‑1 (MQ=255)
                                                                                                                               atgatTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCg  <  3:142738/149‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                             
AACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCG  >  NC_002947/5986707‑5986982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑