| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 441,326 | 0 | G | . | 31.4% | 76.4 / 39.2 | 35 | coding (207/1173 nt) | bioF | 8‑amino‑7‑oxononanoate synthase |
| Reads supporting (aligned to +/- strand): ref base G (15/9); new base . (6/5); total (21/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATCACCGCGCTGGTG > NC_002947/441178‑441473 | aGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGtgt‑g < 8:106086/149‑1 (MQ=255) tGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGGAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATAGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑GGGGGGGGGGGcc > 5:61420/1‑149 (MQ=255) tGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑ggggggggggcct > 3:67585/1‑146 (MQ=255) tGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑ggggggg < 6:207709/143‑1 (MQ=255) tGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑ggggggg > 5:207709/1‑143 (MQ=255) tGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑GGGGGGGGGGGcc > 5:181224/1‑149 (MQ=255) tGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑GGGGGGGGGGGcc > 5:146235/1‑149 (MQ=255) ggAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGtgt‑g > 6:78651/1‑136 (MQ=255) ggAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGtgt‑g < 5:78651/136‑1 (MQ=255) ggAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑GGGGGGGGGGGccg > 6:165174/1‑148 (MQ=255) cGCAAGGGCCGGAAGTGGTGGGCGCCGGCCAGCGGTTTCTGGCCTTCTGCAGAAAATCCTCTCTGGGCCGGGCCAAC‑CCCCCCGCGGTGTTCGCCCCCTGGCAGGCCGGCGCCGCGC‑GC‑GGGGGGG‑GGGGGGGGGGGCCTCGCACCTgg < 8:348597/149‑1 (MQ=255) gCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTGTGCAGCAATGACTACCTGGGCCTGGCCCAC‑CCCCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑GGGGGGGGGGGCCGGGCACCTgg > 8:142224/1‑149 (MQ=255) gggCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑‑GGGGGGGGGGCCGCGCACCTGGTAGtt > 5:251387/1‑149 (MQ=255) ggAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACTAACCCCCAGGGGAAACCCCCCTGGCAGGCCCGCCCCGCGC‑‑CGAGGGGGT‑GGGGGGGGGGGCCACCCCCCGGGttgttgaa > 1:111801/1‑146 (MQ=255) gggcctgggCCCGCGGGGGCGGGCCTTGTTCAGCAAATACAACCGGGGCCGGGCCAAC‑CCCCCCCCGGGTAGTGCCGCCGGGCCCGCCGGGGGCGTTG‑GGTGGGGTGG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCAccc < 6:152941/142‑1 (MQ=255) gtCGACGGCCAGCGGTTGCTGGCCATCTGCAGCAATGACTACCTGGGCCTGGACAAC‑CACCCCGAGGTGATCGACGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGTGGGGGGGGGGGGCCGCCCACGGGGTAGTTGGCCCCAGCGccc > 4:311457/1‑149 (MQ=255) cGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGTGGGGGGGGGGGGCCGCCCCCCGGGTAGTTGGCCACAGccccccc > 4:8252/1‑148 (MQ=255) ccAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGT‑GCTGGGGTGTGGGGGGGGGGGGCGTCGCCCGGGGTAGTTGGCCACAGCCCCCCGCAcaa > 8:126409/1‑146 (MQ=255) cAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGTGGGGGGGGGGGGCCTCGCCCCTGGTAGTTGGCCCCAGCCCCCCGCACCAt > 1:61455/1‑148 (MQ=255) cgggTTGCGGGCCTTCTGCCCCAATGACAACCTGGGCCTGGCCAAC‑CCCCCCGCGGGGTTCGCCCCCTGGCAGGCCGGGGCCCCGC‑GCGGGGGTGG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAgg < 4:304981/147‑1 (MQ=255) cGGTTGCGGGCCTTCTGCACCAATCACAACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGCGG‑GCTGGGGTG‑‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGtt < 4:67585/149‑1 (MQ=255) gCTGGCCTGCGGCACCAAGGCCTACCTGGGCCGGCCCAAC‑CACCCCGTGGGGAGCGCCGCCTGGCAGGCCGGCGCCGCGC‑GCTGGGGGGG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGaa < 3:81218/148‑1 (MQ=255) ccTTTTGCAGCAATGAATACCTGGGCCTGGCAAAC‑CACCCCGTGGTTATCCCCGCCTGGCAGGCGGGCGCCGTGC‑GCTGGGGTGG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCa < 6:181224/149‑1 (MQ=255) ccTTCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCAc > 5:20577/1‑149 (MQ=255) ttCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑‑‑GGGGGGGGGCCTCGCACCTGGTAGTTGGc < 4:101754/112‑1 (MQ=255) ttCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑‑‑GGGGGGGGGCCTCGCACCTGGTAGTTGGc > 3:101754/1‑112 (MQ=255) agcaATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGaa > 7:114371/1‑149 (MQ=255) tACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGgc > 7:20815/1‑149 (MQ=255) cTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCCCCCCGCACCATCAGGTTGAAGAGGCAGTGGCTGAAGTAACCGgccg > 2:252490/1‑149 (MQ=255) ctcgCCCAC‑CACCCCCAGGTGCACCCCGCCCGGCCGGCCGGCGCCGCGC‑GCGGGGGGGG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCcgcg < 6:270451/146‑1 (MQ=255) ggCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑GGGGGGGGGGGCCTCCCACGTGGTAGTTGCCCCCAGCACCCCGCACCAACCGGTTGAAGGGGCCCTGGATGAAAGAACCGGCCCCCcccgcc > 7:163954/1‑148 (MQ=255) cAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑GGGGGGGGGGGCCTCGCCCCGGGTTGTTGGCCACAGCACCCCGCCCCATCAGGGTGAAGGGGCCCTGGCTGAACTCACCGGCCGCCCGCGCGccc > 1:269988/1‑149 (MQ=255) cAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCCCCCCGCACCATCAGGTTGAAGAGGCACTgg > 1:135404/1‑122 (MQ=255) c‑caccCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCtgtt < 6:251387/149‑1 (MQ=255) ccccccGCGGTGTGCGCCGCCGGGCAGGGCGGGGGCGCGG‑GGTGGGGTGT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTAGCTGAACTCACCGGCCGCCCGCGCGCCCtgtt < 6:146235/147‑1 (MQ=255) caccCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCtgttg < 6:20577/149‑1 (MQ=255) cGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑GGGGGGGGGGGCCGCGCCCCTGGTAGTTGGCCCCAGCCCCCCGCACCATCAGGTTGAAGAGGCCCTGGCTGAACTCACCGGCCGCCCGCGCGCCCGgttgttgt > 1:7468/1‑147 (MQ=255) accgccccCTGGCCCGCCGGCGCCGCGCGGGGGGGGGGG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCg < 2:61455/147‑1 (MQ=255) ccTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATgg < 8:114371/149‑1 (MQ=255) cGGCGCCGAGC‑GCTGGGGTGT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTggg > 5:11540/1‑149 (MQ=255) gcCGAGC‑GCTGGGGTGT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGcc < 8:20815/149‑1 (MQ=255) gccgggggggggg‑gggggggGCGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATc < 6:2903/143‑1 (MQ=255) t‑gGGGGGGGGGGCATCGCACATGGTATTTGGCCACAGCACCCCCCCCCATCAGGTTGAAGAGGCACTGGCTGAAATCACCCGCCCCCCGCCCGCCCGGTTTTTAACCACCGGCTATATGGCCAATATTGGCGCCAACACCGCCAtggtg > 8:213233/1‑149 (MQ=255) | AGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAAC‑CACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGC‑GCTGGGGTGT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATCACCGCGCTGGTG > NC_002947/441178‑441473 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |