Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 2,921,460 G→A G3494R (GGG→AGG)  PP_2561 → hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,921,4600GA97.6% 130.0 / ‑7.5 42G3494R (GGG→AGG) PP_2561hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (20/20);  minor base C (0/1);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACC  >  NC_002947/2921328‑2921592
                                                                                                                                    |                                                                                                                                     
gAGGCCGGTAACGACAACATGAACGGCACCGCTGAGGAAGAAAGACAGGATGGGGGGCACTGCAATGACACAATCCAGGGCGGCGATGGTAACGACAGATTGTTCGTAGACGTGGGTACCGCCAGCGGGGTCGGGGGGGCCGGCAGCgg                                                                                                                       >  6:296025/1‑148 (MQ=255)
 ccgCCGGCAACGACACCCGGCCCGGGCGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTATCGACACCCTGATCAGGGGGGCCGGCAACGAc                                                                                                                      <  1:226950/147‑1 (MQ=255)
 aGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGAc                                                                                                                      >  7:279202/1‑149 (MQ=255)
   gCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAAGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACtt                                                                                                                    >  6:233457/1‑149 (MQ=255)
    ccGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTc                                                                                                                   <  5:68546/149‑1 (MQ=255)
      ggTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑Ct                                                                                                                <  8:279202/149‑1 (MQ=255)
       gTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGGTCAGGGGGGCCGGCAACGACTTC‑CTc                                                                                                               <  2:4092/149‑1 (MQ=255)
       gTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCCGCAACGACTTC‑CTc                                                                                                               <  7:317671/149‑1 (MQ=255)
          aCGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAAc                                                                                                            <  3:72177/149‑1 (MQ=255)
            gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCGGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACgg                                                                                                          >  7:206984/1‑149 (MQ=255)
            gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACgg                                                                                                          >  4:6768/1‑149 (MQ=255)
                  cTGAACGGGGGCGCTGGCGATTACAGCCTGGATGGCGGCAACGGCAATGACACCCTGAAGTGCGGCTATGGTAACGACACATTGTTCGGCGACTTGGTTACCGACACCCTGATCAGGTGGGCCGTCAACGACTTC‑CTCAACGGTGCgg                                                                                                     >  6:366460/1‑148 (MQ=255)
                  cTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAAGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGc                                                                                                    <  5:233457/149‑1 (MQ=255)
                     aaCGGGGGCTCTGGCGATGACAGCCTGTATTTCTGCAACTGCAATGACACCCTGCAGGGCTGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAg                                                                                                  <  6:371688/148‑1 (MQ=255)
                        gggggCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACCCCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCCACGGTGCTGCAGGTaa                                                                                              >  1:196066/1‑149 (MQ=255)
                        gggggCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTaa                                                                                              >  1:338743/1‑149 (MQ=255)
                                  gCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGTGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGt                                                                                                <  1:267335/137‑1 (MQ=255)
                                  gCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGt                                                                                                >  2:267335/1‑137 (MQ=255)
                                    gATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACtgtggt                                                                                  >  4:12200/1‑149 (MQ=255)
                                    gATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACggtggt                                                                                  <  5:136870/149‑1 (MQ=255)
                                    gATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACggtggt                                                                                  >  6:136870/1‑149 (MQ=255)
                                    gATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACggtggt                                                                                  <  3:6768/149‑1 (MQ=255)
                                        aCAGCCTGGATGGCGGCAACGGCAATGACACCCTGCTGGGAGGCGATGGTAACGACACATTGATCGGCGACGTGGGCACCGACACCCTGATCCGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTggtgggt                                                                              <  1:219918/149‑1 (MQ=255)
                                             cTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTCTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCGACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGc                                                                         >  4:80684/1‑149 (MQ=255)
                                                gATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCg                                                                       <  2:338743/148‑1 (MQ=255)
                                                 aTGGCGGCAACGGCAATGACACCCTGCAGGGCGTCGATGGTAACGACACATTGTTCGGCGACGTGGGTACGGACACCCTGATCATGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGt                                                                     <  5:366460/149‑1 (MQ=255)
                                                  tGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCagggggg                                                                                                                                 <  5:40156/89‑1 (MQ=255)
                                                  tGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCagggggg                                                                                                                                 >  6:40156/1‑89 (MQ=255)
                                                         aacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCTACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGacac                                                             <  3:80684/149‑1 (MQ=255)
                                                               aaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGAt                                                       >  2:164694/1‑149 (MQ=255)
                                                                     acCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAc                                                 >  7:37838/1‑149 (MQ=255)
                                                                           cAGCGCGGCGATGCTAACGACACATTGCTCGCCGACGTGGGTACCTACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGCGTGCGGCCGGTAACGACACCATGATCGCCACCGAt                                             <  5:296025/147‑1 (MQ=255)
                                                                                  gcgATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAt                                    <  8:206984/149‑1 (MQ=255)
                                                                                                   ttGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTg                    >  1:367164/1‑148 (MQ=255)
                                                                                                   ttGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTg                    >  1:372955/1‑148 (MQ=255)
                                                                                                              cGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCa        <  1:164694/149‑1 (MQ=255)
                                                                                                                tGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAAc      >  4:52540/1‑149 (MQ=255)
                                                                                                                 cgctACCGACACCCTGAACAGGGCGGCCGGCAACCACTTC‑CTCAACGGTGCGGCAGGAAACGATACGGTGGTGGGTGGGGCCGGAAACGACACCAAGATCGCCACCCATGGAAACGATGTGTTCCAGTACGCTGCAGGCTTTCTCAAc      >  8:79828/4‑148 (MQ=255)
                                                                                                                     aCCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAcc  >  4:8497/1‑148 (MQ=255)
                                                                                                                     aCCGACACCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAcc  <  3:8497/148‑1 (MQ=255)
                                                                                                                         acacCCTGATCAGGGGGGCCGGCAACGACTTCGCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCgg                                                                      >  1:233363/1‑77 (MQ=255)
                                                                                                                         acacCCTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCgg                                                                      <  2:233363/76‑1 (MQ=255)
                                                                                                                              cTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAcc  <  6:215125/139‑1 (MQ=255)
                                                                                                                              cTGATCAGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAcc  >  5:215125/1‑139 (MQ=255)
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GAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTC‑CTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACC  >  NC_002947/2921328‑2921592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑