| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 94.7% | 100.3 / ‑6.4 | 38 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base A (16/20); total (16/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 97.3% | 100.8 / NA | 38 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (16/20); total (16/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTGTT‑TCGGG‑CACG‑CCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAG > NC_002947/1499346‑1499611 || ccGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCCCGGTGCAAGGCACC‑GGCTTTGCCGgt > 7:91960/1‑149 (MQ=255) gCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGgtgt < 8:167795/149‑1 (MQ=255) aGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTTAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGttc < 8:173758/149‑2 (MQ=255) cGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGttcgcg < 7:174429/148‑5 (MQ=255) gACCACGCGGAAGCCCGTGCTGCGGCCATTCAGAGCCTGAACGCAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGttcgcgg < 3:356900/148‑6 (MQ=25) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGttcgcgg < 7:32439/148‑6 (MQ=255) aCAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGttcgcgggc > 7:221099/1‑142 (MQ=255) aCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACTGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTTCATGGCACC‑GGCTTTGCCGGTGttcgcgg < 3:193973/144‑6 (MQ=37) aCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACTGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGttcgcgg > 4:193973/1‑139 (MQ=255) gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTTCCCCCGCAAAAGGCGCCCCCGTGCATTGCACC‑CGCTATTCCGGTGTTACCGGG‑CAcc‑cc > 6:134239/1‑149 (MQ=255) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAAAAAGCGCCCCCGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CCCG‑cccccccccc > 4:336869/1‑146 (MQ=21) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCg > 3:327769/1‑120 (MQ=255) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCg < 4:327769/120‑1 (MQ=255) gCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGtt > 1:242970/1‑122 (MQ=255) gCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGtt < 2:242970/122‑1 (MQ=255) gCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACt > 7:276278/1‑148 (MQ=21) ggCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑At > 5:267629/1‑149 (MQ=21) gCCTTTCAGAGCCTGAACGAAGCGTTGGCACCAACGCAGGACTGAGGCTGCCAAACGGGGGGCGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGC‑TGGGCACGGGCTTTGCGGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑Att < 8:221099/149‑1 (MQ=21) gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑Att < 8:276278/149‑1 (MQ=21) cATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGc < 3:84971/149‑1 (MQ=21) agagCCTCAAGTCAGCCTTGCCACCACTCCCGTAGTGAGGCTCCAAAAGGGTGGCTGCGGGCGGGCCCGCTAAACTGGCGCCGGGGTGATTGCCCCC‑GGCTTTGCGGGTGTTCGGGGG‑CAGGCCCGCTCCCACAGACTG‑ATTGCCAtt < 8:17180/148‑1 (MQ=255) ccTGACCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCa < 7:143567/142‑1 (MQ=21) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCAtt < 5:265611/144‑1 (MQ=21) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCAtt > 6:265611/1‑144 (MQ=21) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCg < 5:207128/149‑1 (MQ=21) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCCTGGCCCCGAAACACGGGCCGCGGTGCAATGCACC‑CGCGTTGGCGGTTTTAGCGGG‑CACGCCCGCACCCACACCCAGAATTGCCA‑TAA‑GCggg > 6:322620/1‑149 (MQ=255) cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTAGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCg > 2:267452/1‑149 (MQ=25) gACTGAGGCTGCCAAACGGTGCGAGCGGGCGTGCCCGCGAAACAAGCGCCCCGGTGCATGGCACC‑GGCTTTGCCCCTGTTGGCGGGCCCCC‑CCGCGCCCAAAGACAG‑AATTCCATTAT‑ACCGCCCAATAGCTTCACTGACGGCCCGgtt > 8:203971/1‑149 (MQ=9) tggCTGCCATACGTGGGTAGGGGGCGTGCCCGCGAAACATGCGCGGCGGTGCTTGGCACG‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 7:203971/146‑1 (MQ=21) gCTGCCAAACGGTGGCTGCGGGCGTGCCCGCGAAACAGGCGCGGCGGTGCATGGCACC‑GGCTTTGCCGGTTTTCGCGGT‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg < 6:279518/149‑1 (MQ=21) cGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa < 3:336869/149‑1 (MQ=21) aGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 6:267629/149‑1 (MQ=25) gggggCGTGCCGGGGAAACTGGCGCGGCGGTGCTTGGCAGC‑GGCTTTGCGGGTGTTCGCGGT‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAATGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc < 1:97227/146‑1 (MQ=255) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACt > 8:161259/1‑149 (MQ=25) cccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 1:181507/1‑148 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 2:321829/1‑147 (MQ=37) cgccgcGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCaa < 1:321829/149‑1 (MQ=37) gccgcGGTGCATGGCACC‑GGCTTTGCCGGTGTTCGCGGG‑CACGCCCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg < 1:267452/149‑1 (MQ=37) || CCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTGTT‑TCGGG‑CACG‑CCGCTCCCACAGACTG‑ATTGCCATTAA‑GCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAG > NC_002947/1499346‑1499611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |