Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,348,718 Δ1 bp 9.3% coding (1192/2937 nt) PP_4704 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,348,7180A. 9.3% 129.9 / 7.9 43coding (1192/2937 nt)PP_4704hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (19/20);  new base . (2/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

CAGAACCTACGCCTGAAGAGAAAGCTGCCAAGGAGCCAAGCAAACCCTGGCAAGTGCTGCTCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTGCAGGCCGCAGGCCAGGTCAACCTGGCACCGATATGGGCAAAGCTGGATGTGAGCAC  >  NC_002947/5348572‑5348860
                                                                                                                                                  |                                                                                                                                              
cAGAACCTACGCCTGAAGAGAAAGCTGCCAAGGAGCCAAGCAAACCCTGGCAAGTGCTGCTCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAAc                                                                                                                                              <  2:229618/149‑1 (MQ=255)
         cGCCTGAAGAGAAAGCTGCCAAGGAGCCAAGCAAACCCTGGCAAGTGCTGCTCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTATCCTTGGACATTGGCCCCCTCAACGCCGACCt                                                                                                                                      <  1:249826/148‑1 (MQ=255)
                    aaaGCTGCCAAGGAGCCAAGCAAACCCTGGCAAGTGCTGCTCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGa                                                                                                                          >  5:67123/1‑149 (MQ=255)
                     aaGCTGCCATGGAGCCAAGCAAAGCCTGGCAAGTGCTGCTCAAGGACGTGCAGTTGCGCAACTACCTGGTGTATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGa                                                                                                                          <  6:188320/148‑1 (MQ=255)
                           ccAAGGAGCCAAGCAAACCCTGGCAAGTGCTGCTCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCt                                                                                                                    >  2:11437/1‑148 (MQ=255)
                              aGGAGCCAAGCAAACCCTGGCAAGTGCTGCTCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAAc                                                                                                                >  1:230705/1‑149 (MQ=255)
                              aGGAGCCAAGCAAACCCTGGCAAGTGCTGCTCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAAc                                                                                                                <  6:67123/149‑1 (MQ=255)
                                     aaGCAAACCCTGGCAAGTGCTGCTCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCaa                                                                                                                 <  6:369056/141‑1 (MQ=255)
                                     aaGCAAACCCTGGCAAGTGCTGCTCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCaa                                                                                                                 >  5:369056/1‑141 (MQ=255)
                                     aaGCAAACCCTGGCAAGTGCTGCTCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGc                                                                                                         >  2:260219/1‑149 (MQ=255)
                                      aGCAAACCCTGGCAAGTGCTGCTCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGCTCGCAGCCAGTAGGACCCGGTAGCCTTGGACATTGGCCCCCTGAACGCCGCCCTGCAAGGTTTCGACACGCTCAACCAGGCGcc                                                                                                        >  8:388603/1‑149 (MQ=255)
                                       gCAAACCCTGGCAAGTGCTGCTCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGccc                                                                                                       <  2:230705/149‑1 (MQ=255)
                                              cTGGCAAGTGCTGCTCAAGGACGGGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCAccc                                                                                                <  7:44292/149‑1 (MQ=255)
                                               tGGCAAGTGCTGCTCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGt                                                                                                                               >  2:340845/1‑117 (MQ=255)
                                               tGGCAAGTGCTGCTCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGt                                                                                                                               <  1:340845/117‑1 (MQ=255)
                                                           ctCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGAc                                                                                                                         >  6:170402/1‑111 (MQ=255)
                                                           ctCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGAc                                                                                                                         <  5:170402/111‑1 (MQ=255)
                                                            tCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTC‑ACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGa                                                                                      >  6:151011/1‑144 (MQ=255)
                                                            tCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTC‑ACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGa                                                                                      >  6:144592/1‑144 (MQ=255)
                                                            tCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTC‑ACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGa                                                                                      <  5:144592/144‑1 (MQ=255)
                                                            tCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTC‑ACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGa                                                                                      <  5:151011/144‑1 (MQ=255)
                                                                  aCGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGtgtg                                                                            <  5:378752/149‑1 (MQ=255)
                                                                       cAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGgcaa                                                                       <  1:11437/149‑1 (MQ=255)
                                                                                     ccTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTg                                                          <  1:260219/148‑1 (MQ=255)
                                                                                       tGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGgcaa                                                                       <  1:258054/133‑1 (MQ=255)
                                                                                       tGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGgcaa                                                                       >  2:258054/1‑133 (MQ=255)
                                                                                                tGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTgcaggccgcaggc                                              >  6:357962/1‑149 (MQ=255)
                                                                                                  gCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTgcaggccgcaggc                                              >  2:269622/1‑147 (MQ=255)
                                                                                                               aGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTGCAGGCCGCAGGCCAGGTCAACCTGGCa                               >  8:311455/1‑149 (MQ=255)
                                                                                                                        cGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAACCTCGATACCGGTGTGGGCAAGCAAGGCAAGCTGCAGGCCGCAGGCCAGGTCAACCTGGCACCGATATgg                      <  7:388603/149‑1 (MQ=255)
                                                                                                                               cTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTc                                                                                        <  8:216487/76‑1 (MQ=255)
                                                                                                                               cTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTc                                                                                        >  7:216487/1‑76 (MQ=255)
                                                                                                                                ttGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGgcaag                                                                      <  3:90228/93‑1 (MQ=255)
                                                                                                                                ttGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGgcaag                                                                      >  4:90228/1‑93 (MQ=255)
                                                                                                                                ttGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTGCAGGCCGCAGGCCAGGTCAACCTGGCACCGATATGGGCAAAGCt              >  1:310242/1‑149 (MQ=255)
                                                                                                                                ttGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTGCAGGCCGCAGGCCAGGTCAACCTGGCACCGATATGGGCAAAGCt              >  5:109246/1‑149 (MQ=255)
                                                                                                                                     cATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTGCAGGCCGCAGGCCAGGTCAACCTGGCACCGATATGGGCAAAGCTGGAtg         <  7:250927/149‑1 (MQ=255)
                                                                                                                                      aTTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTGCAGGCCGCAGGCCAGGTCAACCTGGCACCGatat                        >  8:129876/1‑133 (MQ=255)
                                                                                                                                      aTTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTGCAGGCCGCAGGCCAGGTCAACCTGGCACCGatat                        <  7:129876/133‑1 (MQ=255)
                                                                                                                                         ggCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTGCAGGCCGCAGGCCAGGTCAACCTGGCACCGATATGGGCAAAGCTGGATGTGa      <  5:357962/148‑1 (MQ=255)
                                                                                                                                            ccccTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTGCAGGCCGCAGGCCAGGTCAACCTGGCACCGATATGGGCAAAGCTGGATGTGAGCAc  >  7:200870/1‑149 (MQ=255)
                                                                                                                                             cccTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTgcaggccgca                                                 >  3:301149/1‑101 (MQ=255)
                                                                                                                                             cccTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTgcaggccgca                                                 <  4:301149/101‑1 (MQ=255)
                                                                                                                                                  aaCGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTgcagg                                                      <  3:383045/91‑1 (MQ=255)
                                                                                                                                                  aaCGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTgcagg                                                      >  4:383045/1‑91 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CAGAACCTACGCCTGAAGAGAAAGCTGCCAAGGAGCCAAGCAAACCCTGGCAAGTGCTGCTCAAGGACGTGCAGTTGCGCAACTACCTGGTGCATCTGGCAGATCGCAGCCAGAAGGAACCGGTAGCCTTGGACATTGGCCCCCTCAACGCCGACCTGCAAGGTTTCGACAGCCTCAACCAGTCGCCCTTCACCCTCAAACTCGATACCGGTGTGGGCAAGCAAGGCAAGCTGCAGGCCGCAGGCCAGGTCAACCTGGCACCGATATGGGCAAAGCTGGATGTGAGCAC  >  NC_002947/5348572‑5348860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑