Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,463,407 Δ1 bp 6.7% intergenic (‑58/+41) PP_4799 ← / ← lipA muramoyltetrapeptide carboxypeptidase/lipoyl synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,463,4070C. 6.7% 244.1 / 10.5 75intergenic (‑58/+41)PP_4799/lipAmuramoyltetrapeptide carboxypeptidase/lipoyl synthase
Reads supporting (aligned to +/- strand):  ref base C (32/38);  new base . (2/3);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

GCGCCCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAA  >  NC_002947/5463264‑5463544
                                                                                                                                                |                                                                                                                                         
gcgccCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGCCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATc                                                                                                                                      >  3:262261/1‑149 (MQ=255)
 cgcccgccGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCg                                                                                                                                     >  7:223237/1‑149 (MQ=255)
   cccgccGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGc                                                                                                                                   <  8:52567/149‑1 (MQ=255)
    ccgccgGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCGCGCCCAAGGGCGGCATCGGCa                                                                                                                                  <  3:30990/149‑1 (MQ=255)
    ccgccgGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCg                                                                                                                                     <  1:212364/146‑1 (MQ=255)
    ccgccgGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCg                                                                                                                                     >  2:212364/1‑146 (MQ=255)
    ccgccgGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGc                                                                                                                                   >  6:280282/1‑148 (MQ=255)
        cgGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCtg                                                                                                                              <  7:334922/149‑1 (MQ=255)
        cgGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCtg                                                                                                                              <  7:208493/149‑1 (MQ=255)
          gggCGACAATGGCAAACCCCGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCtgtg                                                                                                                            <  3:287383/149‑1 (MQ=255)
            gCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCGCGCCCAAGGGCGGCATCGGCa                                                                                                                                  >  8:347219/1‑141 (MQ=255)
            gCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATCCCGATGCGCGCCCAAGGGCGGCATCGGCa                                                                                                                                  <  7:347219/141‑1 (MQ=255)
              gACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTc                                                                                                                        >  2:202072/1‑149 (MQ=255)
              gACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCAATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTc                                                                                                                        >  3:209705/1‑149 (MQ=255)
                    ggCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGctc                                                                                                                   >  2:55231/1‑148 (MQ=255)
                     gCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTa                                                                                                                 <  5:280282/149‑1 (MQ=255)
                      cAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAg                                                                                                                >  2:34108/1‑149 (MQ=255)
                       aaaaCATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTgg                                                                                                                           >  1:189809/1‑137 (MQ=255)
                       aaaaCATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTgg                                                                                                                           <  2:189809/137‑1 (MQ=255)
                            aTGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTaga                                                                                                          <  1:55231/149‑1 (MQ=255)
                            aTGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTa                                                                                                            >  2:252361/1‑147 (MQ=255)
                            aTGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTa                                                                                                            >  2:178558/1‑147 (MQ=255)
                             tGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGct                                                                                                                    <  3:17936/138‑1 (MQ=255)
                             tGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAAAAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGct                                                                                                                    >  4:17936/1‑138 (MQ=255)
                                    gCCGTCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCt                                                                                                  <  8:102540/149‑1 (MQ=255)
                                    gCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCt                                                                                                  >  6:297281/1‑149 (MQ=255)
                                        gCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCa                                                                                                >  3:177477/1‑147 (MQ=255)
                                        gCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCa                                                                                                >  3:333718/1‑147 (MQ=255)
                                               ggCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCAATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGAt                                                                                       <  4:209705/149‑1 (MQ=255)
                                                    gCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCtt                                                                                    <  2:219076/147‑1 (MQ=255)
                                                    gCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCtt                                                                                    <  8:364913/147‑1 (MQ=255)
                                                    gCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTg                                                                                   <  7:158822/148‑1 (MQ=255)
                                                       gggtgggtTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGAAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCtt                                                                               >  3:94811/1‑149 (MQ=255)
                                                        ggtgggtTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTc                                                                              <  8:223237/149‑1 (MQ=255)
                                                         gtgggtTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCg                                                                             <  2:50869/149‑1 (MQ=255)
                                                         gtgggtTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCg                                                                             <  3:178744/149‑1 (MQ=255)
                                                            ggtTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTgg                                                                          <  1:202072/149‑1 (MQ=255)
                                                              ttGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGc                                                                        <  4:333718/149‑1 (MQ=255)
                                                                 gAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCctg                                                                     <  2:42407/149‑1 (MQ=255)
                                                                   aGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCctgct                                                                   >  5:252210/1‑149 (MQ=255)
                                                                    ggTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCctgctg                                                                  <  1:178558/149‑1 (MQ=255)
                                                                    ggTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACAAAAACTAAACTGCCGATGCCGCCCTTGTGCGCGCATCGGCATGTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCctgctg                                                                  <  7:348913/149‑1 (MQ=255)
                                                                      ttttGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTCGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGt                                                                >  2:186810/1‑149 (MQ=255)
                                                                        ttGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGCGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTc                                                               <  1:252361/148‑1 (MQ=255)
                                                                             gcAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTg                                                         <  1:320798/149‑1 (MQ=255)
                                                                                gTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGt                                                                            <  2:173287/127‑1 (MQ=255)
                                                                                gTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTa                                                      >  3:139273/1‑149 (MQ=255)
                                                                                gTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAAAGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGt                                                                            >  1:173287/1‑127 (MQ=255)
                                                                                 tACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGt                                                       >  1:367703/1‑147 (MQ=255)
                                                                                   cATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACg                                                    >  1:379833/1‑148 (MQ=255)
                                                                                       gcgGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGTCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACgaagaa                                               <  4:262261/149‑1 (MQ=255)
                                                                                       gcgGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACgaaga                                                <  5:297281/148‑1 (MQ=255)
                                                                                           cTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCa                                           >  3:216878/1‑149 (MQ=255)
                                                                                              cTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCAc                                          <  1:34108/147‑1 (MQ=255)
                                                                                               ttGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGc                                                                        <  2:408069/116‑1 (MQ=255)
                                                                                               ttGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGc                                                                        >  1:408069/1‑116 (MQ=255)
                                                                                               ttGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAg                                       >  8:185180/1‑149 (MQ=255)
                                                                                                         ttGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGaa                             <  6:252210/149‑1 (MQ=255)
                                                                                                         ttGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGaa                             <  2:333769/149‑1 (MQ=255)
                                                                                                             acacAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCaa                         >  4:168466/1‑149 (MQ=255)
                                                                                                              cacaAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAAc                        <  4:216878/149‑1 (MQ=255)
                                                                                                               acaaacaaAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACg                       >  5:99636/1‑149 (MQ=255)
                                                                                                                 aaacaaaAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTgg                                                                          <  6:243750/96‑1 (MQ=255)
                                                                                                                 aaacaaaAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTgg                                                                          >  5:243750/1‑96 (MQ=255)
                                                                                                                 aaacaaaAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACgaa                                                  <  4:112121/120‑1 (MQ=255)
                                                                                                                 aaacaaaAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACgaa                                                  >  3:112121/1‑120 (MQ=255)
                                                                                                                 aaacaaaAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCa                                <  4:254925/138‑1 (MQ=255)
                                                                                                                 aaacaaaAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCa                                >  3:254925/1‑138 (MQ=255)
                                                                                                                   acaaaAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCt                   >  3:391622/1‑149 (MQ=255)
                                                                                                                    caaaAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCtt                  <  2:379833/149‑1 (MQ=255)
                                                                                                                     aaaaaTGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTg                 <  4:94811/149‑1 (MQ=255)
                                                                                                                        gatgccgatgcgcGCCCAAGGGCGGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTg                 >  8:382471/2‑146 (MQ=255)
                                                                                                                        gatgccgatgcgcGCCCAAGGGCGGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGaa               <  7:382471/147‑1 (MQ=255)
                                                                                                                                  ccgccCTCGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGt    <  1:186810/149‑1 (MQ=255)
                                                                                                                                     gccCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGTGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTaa  >  2:352109/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                         
GCGCCCGCCGGGGCGACAATGGCAAAACATGCGTTGGCCGGCAGCGCGGCTGGCAGGGTGGGTTGGAAGGTTTTGGCGCAGTACATCGCGGCTCCTTGCAGCGCATTGGACACAAACAAAAATGCCGATGCCGCCCTTGGGCGCGCATCGGCATCTGTGGCTCCGCTCTAGGCTAGAGCATAGCTCAGAGCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAA  >  NC_002947/5463264‑5463544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: