Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,385,793	Δ1 bp	6.9%	coding (85/576 nt)	PP_2093 ←	two‑component system response regulator NasT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,385,793	0	T	.	6.9%	175.1 / 7.5	58	coding (85/576 nt)	PP_2093	two‑component system response regulator NasT
Reads supporting (aligned to +/- strand):  ref base T (26/28);  new base . (2/2);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

AACACGATGGGCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAA  >  NC_002947/2385645‑2385940                                                                                                                                                     |                                                                                                                                                      aacaCGATGGGCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCgat                                                                                                                                                       <  3:16362/149‑1 (MQ=255)  acacGATGGGCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCgat                                                                                                                                                       >  4:317240/1‑148 (MQ=255)  acacGATGGGCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCgat                                                                                                                                                       <  3:317240/148‑1 (MQ=255)    acGATGGGCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCgatgat                                                                                                                                                    <  6:208419/149‑1 (MQ=255)      gATGGGCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAt‑c‑t                                                                                                                                                >  7:157243/1‑149 (MQ=255)           gCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGA‑GAT‑CATCGAAg                                                                                                                                           <  7:147192/149‑1 (MQ=255)           gCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTACATCACATCGCGGTCTGGCGAGTCGGTATCGATAAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAg                                                                                                                                           >  7:258712/1‑149 (MQ=255)              gcgGCTGGTCACGGCTGCCCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCg                                                                                                                                        >  5:337555/1‑149 (MQ=255)               cgGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCgg                                                                                                                                       <  8:293651/149‑1 (MQ=255)                          ggCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAAc                                                                                                                            <  7:339772/149‑1 (MQ=255)                            cTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAG‑C‑TCGACGCCGGCCTCACGCACCGc                                                                                                                          >  6:115496/1‑149 (MQ=255)                             tGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACg                                                                                                                           >  2:43235/1‑147 (MQ=255)                               aCCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAg                                                                                                                        <  4:329361/148‑1 (MQ=255)                               aCCAGCACTACCTGCTCCATCAGATCGCGGTCTGGCGAGTCGGAATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCa                                                                                                                         <  7:48994/147‑1 (MQ=255)                                 cAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCg                                                                                                                                        <  8:318897/130‑1 (MQ=255)                                 cAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCg                                                                                                                                        >  7:318897/1‑130 (MQ=255)                                   gCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTc                                                                                                                   <  6:370351/149‑1 (MQ=255)                                       tACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGc                                                                                                               >  7:226719/1‑149 (MQ=255)                                         ccTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAt‑c                                                                                                                                                  >  5:53146/1‑113 (MQ=255)                                         ccTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAt‑c                                                                                                                                                  <  6:53146/113‑1 (MQ=255)                                         ccTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGa                                                                                                             >  2:101940/1‑149 (MQ=255)                                            gTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAgg                                                                                                                <  2:353482/143‑1 (MQ=255)                                            gTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAgg                                                                                                                >  1:353482/1‑143 (MQ=255)                                             ttCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGAcc                                                                                                           >  1:184879/1‑147 (MQ=255)                                                 aTCACGTCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACAAACCCCTCAGACGACAGATCtt                                                                                                     >  6:290193/1‑149 (MQ=255)                                                 aTCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCtt                                                                                                     <  2:253279/149‑1 (MQ=255)                                                  tCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTc                                                                                                                   <  4:263893/134‑1 (MQ=255)                                                  tCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTc                                                                                                                   >  3:263893/1‑134 (MQ=255)                                                      aTCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGctct                                                                                                >  6:17345/1‑149 (MQ=255)                                                         gcgGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATCC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTg                                                                                               <  1:101940/148‑1 (MQ=255)                                                         gcgGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTgg                                                                                              <  8:226719/148‑1 (MQ=255)                                                             tCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTgtc                                                                                         <  2:184879/149‑1 (MQ=255)                                                               gggCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTgtcgt                                                                                       <  4:283320/148‑1 (MQ=255)                                                                         gTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATcagcag                                                                             >  6:67168/1‑149 (MQ=255)                                                                         gTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGA‑GAT‑CATCGAAGcccca                                                                                                                                      >  2:410172/1‑89 (MQ=38)                                                                         gTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGA‑GAT‑CATCGAAGcccca                                                                                                                                      <  1:410172/92‑4 (MQ=38)                                                                              gATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATgc                                                                        <  8:157243/149‑1 (MQ=255)                                                                                  gtatccacGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCaa                                                                    >  4:130028/5‑149 (MQ=255)                                                                                   gCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCaa                                                                    >  1:41065/1‑148 (MQ=255)                                                                                   gCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAAc                                                                   <  2:41065/149‑1 (MQ=255)                                                                                     accacGTCCGGGCGCACCGTTGCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCTGCCTCAGTCAACGGAGTGCTCAGGCGACTGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACAt                                                                 <  5:290193/149‑1 (MQ=255)                                                                                      ccacGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATg                                                                >  7:42103/1‑149 (MQ=255)                                                                                       cacGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGc                                                                                                       <  4:307760/109‑1 (MQ=255)                                                                                       cacGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGc                                                                                                       >  3:307760/1‑109 (MQ=255)                                                                                       cacGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTgg                                                                                              >  3:290735/1‑118 (MQ=255)                                                                                       cacGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTgg                                                                                              <  4:290735/118‑1 (MQ=255)                                                                                        acGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGtt                                                              <  5:67168/149‑1 (MQ=255)                                                                                          gTCCGGGCGCACCGTTTCGACGCGGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCg                                                            >  5:227499/1‑149 (MQ=255)                                                                                               ggCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTcccc                                                       <  8:42103/149‑1 (MQ=255)                                                                                                        tttCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACgcgc                                              <  8:258712/149‑1 (MQ=255)                                                                                                            gACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGac                                          >  4:323582/1‑149 (MQ=255)                                                                                                                         aGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCaa                                                                    <  2:347159/110‑1 (MQ=255)                                                                                                                         aGGTCGATGGTCAGGGCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCGCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGc                             <  6:337555/149‑1 (MQ=255)                                                                                                                                   tCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTccccc                                                      >  7:79978/1‑114 (MQ=255)                                                                                                                                   tCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTccccc                                                      <  8:79978/114‑1 (MQ=255)                                                                                                                                   tCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGccc                    >  8:174854/1‑148 (MQ=255)                                                                                                                                   tCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCACCGGGCAACTCGccc                    >  3:176959/1‑148 (MQ=255)                                                                                                                                     aGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAg                 <  1:43235/149‑1 (MQ=255)                                                                                                                                         ggccggCTTCGA‑GAT‑CATCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCAt             >  7:224520/5‑149 (MQ=255)                                                                                                                                          tGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATg            >  6:205841/1‑149 (MQ=255)                                                                                                                                            gggCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGc           <  3:130028/148‑1 (MQ=255)                                                                                                                                              gCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAg        <  5:17345/149‑1 (MQ=255)                                                                                                                                                    atgat‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTGCGTCCCCCTCACGCGCCGACACGGGCAGCGGGCAACTCGCCCAAGGCATGCAAGCTGaaa  <  5:115496/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                      AACACGATGGGCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAT‑C‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAA  >  NC_002947/2385645‑2385940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑