Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,114,318 Δ1 bp 8.3% intergenic (+65/+81) PP_3619 → / ← ycaO hypothetical protein/ribosomal protein S12 methylthiotransferase accessory factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,114,3180T. 8.3% 142.2 / 7.4 48intergenic (+65/+81)PP_3619/ycaOhypothetical protein/ribosomal protein S12 methylthiotransferase accessory factor
Reads supporting (aligned to +/- strand):  ref base T (27/17);  new base . (2/2);  total (29/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

CTGTGTCTGCAAGCTTTTCAATGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCTTGTAGCTGTCGATCAGGCGCAGGTGGCGATCAA  >  NC_002947/4114172‑4114466
                                                                                                                                                  |                                                                                                                                                      
cTGTGTCTGCAAGCTTTTCAATGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTc                                                                                                                                                       <  5:172948/148‑1 (MQ=255)
  gtgtCTGCAAGCTTTTCAATGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCca                                                                                                                                                     >  7:172056/1‑148 (MQ=255)
   tgtCTGCAAGCTTTTCAATGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCca                                                                                                                                                     >  5:310605/1‑147 (MQ=255)
         cAAGCTTTTCAATGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGtt                                                                                                                                               <  2:2318/147‑1 (MQ=255)
          aaGCTTTTCAATGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCgg                                                                                                                                            >  4:389967/1‑149 (MQ=255)
                ttCAATGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGAAAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAg                                                                                                                                       <  7:75020/148‑1 (MQ=255)
                   aaTGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGAtct                                                                                                                                   >  4:87199/1‑149 (MQ=255)
                    aTGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGAtctc                                                                                                                                  <  3:389967/149‑1 (MQ=255)
                       tCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGAtctc                                                                                                                                  >  5:18356/1‑146 (MQ=255)
                         cGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAagag                                                                                                                             <  6:18356/149‑1 (MQ=255)
                              aGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAg                                                                                                                                                  >  8:10841/1‑123 (MQ=255)
                              aGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAg                                                                                                                                                  <  7:10841/123‑1 (MQ=255)
                              aGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTg                                                                                                                        <  8:172056/149‑1 (MQ=255)
                                    accaccGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATAc                                                                                                                  <  8:170462/149‑1 (MQ=255)
                                     ccaccGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCCTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATAcc                                                                                                                 >  5:13094/1‑149 (MQ=255)
                                      caccGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCt                                                                                                                <  3:87199/149‑1 (MQ=255)
                                           gcggAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCG‑CCACAGGTATTGCACCGCTCTTGAGATCTGTGCCGAACCTGTggac                                                                                                          >  7:311347/1‑147 (MQ=21)
                                              gAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCG‑CCACAGGTATTGCACCGCTCTTGAGATCTGTGCCGAACCTGTggacgct                                                                                                       <  8:311347/149‑6 (MQ=18)
                                               aGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTg                                                                                                      <  6:310605/149‑1 (MQ=255)
                                                      cGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGcc                                                                                               >  1:98521/1‑149 (MQ=255)
                                                                    cTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGgc                                                                                 >  6:378922/1‑149 (MQ=255)
                                                                    cTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGgc                                                                                 >  2:238417/1‑149 (MQ=255)
                                                                     tAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCaa                                                                                                                                <  8:357772/102‑1 (MQ=255)
                                                                     tAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCaa                                                                                                                                >  7:357772/1‑102 (MQ=255)
                                                                     tAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGgcg                                                                                >  1:277566/1‑149 (MQ=255)
                                                                            gAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGcc                                                                         >  8:207496/1‑149 (MQ=255)
                                                                                  tCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACTGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAg                                                                   >  1:73704/1‑149 (MQ=255)
                                                                                  tCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAg                                                                   >  2:263020/1‑149 (MQ=255)
                                                                                       agcTGATGTGTTTAGGATGATACCGCTATGGGCGTGGATGCCAACGCGCCACGCCAGGGTCCAGTGTTGCGCCACAGTTCTCAACAGCGGTGAAAAACGTGTGG‑CGGTGGGTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGaa                                                              <  8:244043/147‑1 (MQ=255)
                                                                                       accTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCCGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTCCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGaa                                                              <  5:207919/149‑1 (MQ=255)
                                                                                        ccTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAAc                                                             <  5:378922/149‑1 (MQ=255)
                                                                                         cTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACt                                                            >  5:192215/1‑149 (MQ=255)
                                                                                         cTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGAGATGATTGGGCGCAGCGCCTGTCAGGCGAACt                                                            >  6:290323/1‑149 (MQ=255)
                                                                                                                 tATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGgc                                                                                 >  3:396666/1‑104 (MQ=255)
                                                                                                                   tGCGCCTGGAGGGCAACGCGGCAAGCCAGCGTCCACAGTTTCGCCACATATCTCAAGAGCGTTGCAATACCTGGGG‑CGCGGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGcttctt                                  <  2:98521/148‑1 (MQ=255)
                                                                                                                      gcCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCTTGTAg                              <  6:192215/149‑1 (MQ=255)
                                                                                                                       ccTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCCTG‑CAGCTTATTg                                 >  3:223043/1‑145 (MQ=255)
                                                                                                                          ggATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCTTGTAGCTGt                          >  7:220751/1‑149 (MQ=255)
                                                                                                                                cAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCTTGTAGCTGTCGAt                      >  5:364591/1‑147 (MQ=255)
                                                                                                                                 aaCGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCt                                                                        >  5:161823/1‑97 (MQ=255)
                                                                                                                                  aCGCGGCAACCCAGCGTCCACACGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTCCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCTTGTAGCTGTCGATCAgg                  >  1:180693/1‑149 (MQ=255)
                                                                                                                                    gcgGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCTTGTAGCTGTCGATCAGgcg                >  5:233105/1‑149 (MQ=255)
                                                                                                                                      ggCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCTTGTAGCTGTCGATCAGgcg                >  7:52477/1‑147 (MQ=255)
                                                                                                                                       gCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTGCCAGCTTCTTGTAGCTGTCGATCAGGCGCa              <  1:238417/149‑1 (MQ=255)
                                                                                                                                         aaGCCAGCG‑CCACAGGTATTGCACCGCTCTTGAGATCTGTGCCGAACCTGTGGACGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCTTGTAGCTGt                          <  4:358230/134‑1 (MQ=14)
                                                                                                                                         aaGCCAGCG‑CCACAGGTATTGCACCGCTCTTGAGATCTGTGCCGAACCTGTGGACGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCTTGTAGCTGt                          >  3:358230/1‑134 (MQ=14)
                                                                                                                                            ccAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCTTGTAGCTGTCGATCAGGCGCAGGTGGc        >  8:46713/1‑149 (MQ=255)
                                                                                                                                                cGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCTTGTAGCTGTCGATCAGGCGCAGGTGGCGAt     >  4:119993/1‑148 (MQ=255)
                                                                                                                                                  tCCACAGGTTCGGCACAGATATCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGTCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCTTGTAGCTGTCGATCAGGCGCAGGTGGCGATCaa  <  3:317193/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
CTGTGTCTGCAAGCTTTTCAATGTCCGTGCAGCGCCACCACCGGCGGAGGCTTTCGAAAGTGGGCCTGCTAAGCCCGAATAGTCACCACCTGAAGTGGATAGGAAGAGAACGCTATGCGCCTGGATGGCAACGCGGCAAGCCAGCGTCCACAGGTTCGGCACAGATCTCAAGAGCGGTGCAATACCTGTGG‑CGCTGGCTTGCCGGCGATGATTGGGCGCAGCGCCTGTCAGGCGAACTTGGCCCGGGCCGCGTG‑CAGCTTCTTGTAGCTGTCGATCAGGCGCAGGTGGCGATCAA  >  NC_002947/4114172‑4114466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑