Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	644,641:1	+G	5.6%	coding (376/978 nt)	acoA ←	acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	644,641	1	.	G	5.6%	291.5 / 11.1	72	coding (376/978 nt)	acoA	acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit alpha
Reads supporting (aligned to +/- strand):  ref base . (37/31);  new base G (2/2);  total (39/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

CTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGCCATCATGCCGTACAC  >  NC_002947/644495‑644779                                                                                                                                                    |                                                                                                                                           ctcGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCAAGCGCCGCCCCGGc‑ca                                                                                                                                          >  3:58531/1‑149 (MQ=255)       cACGAAGATGCAGGGCTGGTTCATGATCGACGCCAGGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCGTAAGAAGGCTACGGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAgtg                                                                                                                                    <  1:102810/149‑1 (MQ=255)       cACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAgtg                                                                                                                                    <  3:74975/149‑1 (MQ=255)        aCGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCGCACCActg                                                                                                                                    >  8:122669/1‑149 (MQ=255)         cGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAgtggt                                                                                                                                  <  4:163963/149‑1 (MQ=255)          gAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAgtggtg                                                                                                                                 >  3:2691/1‑149 (MQ=255)           aaGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCAAGCGCCGCCCCGGC‑CACCAGTGGTgc                                                                                                                                <  4:58531/149‑1 (MQ=255)                  agggcaggTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCGCACCACTGGtg‑gccgggg                                                                                                                         <  7:122669/149‑3 (MQ=255)                  agggcaggTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTgcgccggcg                                                                                                                         >  8:330009/1‑149 (MQ=255)                  agggcaggTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTgcgccggcg                                                                                                                         >  1:48848/1‑149 (MQ=255)                      caggTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAgtg                                                                                                                                    >  1:231087/1‑134 (MQ=255)                      caggTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAgtg                                                                                                                                    <  2:231087/134‑1 (MQ=255)                         gTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCcgacga                                                                                                                  >  6:164712/1‑149 (MQ=255)                         gTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCcgacga                                                                                                                  <  7:163961/149‑1 (MQ=255)                               gATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGt                                                                                                            >  2:20895/1‑149 (MQ=255)                               gATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGt                                                                                                            >  2:14599/1‑149 (MQ=255)                                aTCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGtt                                                                                                           <  7:330009/149‑1 (MQ=255)                                    aCGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAgtggt                                                                                                                                  <  1:152763/122‑1 (MQ=255)                                    aCGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAgtggt                                                                                                                                  >  2:152763/1‑122 (MQ=255)                                       ccATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCAcc                                                                                                     >  7:225890/1‑148 (MQ=255)                                        cATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCAcc                                                                                                     >  1:385826/1‑147 (MQ=255)                                         aTGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGATTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAg                                                                                                  >  7:304695/1‑149 (MQ=255)                                                    cTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCtttt                                                                                       >  7:16777/1‑149 (MQ=255)                                                     tCGAATACGGCGACCTCGTTTGATGCCACATCGACGAAGAAGGCTACCGAAACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGACGCCACGGC‑CCCCAgtg                                                                                                                                    >  5:228933/1‑103 (MQ=255)                                                     tCGAATACGGCGACCTCGTTGTATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCATTGAGCTTGGCAGCCAGCTCCGCCCCGGC‑CACCAgtg                                                                                                                                    <  6:228933/103‑1 (MQ=255)                                                       gAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCCCCGAGCATGCCCGTTTCCa                                                                                    >  4:168668/1‑149 (MQ=255)                                                       gAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTc                                                                                      >  2:347081/1‑147 (MQ=255)                                                        aaTACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATAcc                                                                                                              <  1:344585/122‑1 (MQ=255)                                                        aaTACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATAcc                                                                                                              >  2:344585/1‑122 (MQ=255)                                                        aaTACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAg                                                                                   <  8:21034/149‑1 (MQ=255)                                                             ggCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCgg                                                                              >  7:286123/1‑149 (MQ=255)                                                              gcgcCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCgg                                                                              >  6:121724/1‑148 (MQ=255)                                                                  ccTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCGCACCACTGGtg‑gccggggc                                                                                                                        >  7:114216/1‑102 (MQ=255)                                                                  ccTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCGCACCACTGGtg‑gccggggc                                                                                                                        <  8:114216/102‑1 (MQ=255)                                                                  ccTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAAtg                                                                         >  6:190415/1‑149 (MQ=255)                                                                   cTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAAtgt                                                                        <  5:190415/149‑1 (MQ=255)                                                                         ggATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATc                                                                   <  3:359147/148‑1 (MQ=255)                                                                          gATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGAAATGTGCATCGa                                                                 >  4:132408/1‑149 (MQ=255)                                                                              ccccATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAgccg                                                             <  8:225890/149‑1 (MQ=255)                                                                               cccATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCg                                                                                                             <  5:22161/100‑1 (MQ=255)                                                                               cccATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCg                                                                                                             >  6:22161/1‑100 (MQ=255)                                                                                   tCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCttt                                                        >  6:396993/1‑149 (MQ=255)                                                                                     gCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGTAATGTGCATCGAGCCGCCTTTGc                                                      <  3:132408/149‑1 (MQ=255)                                                                                      ccGAAGAAGGCTACCGACACGTCATTGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCtt                                                                                         <  1:154675/113‑1 (MQ=255)                                                                                      ccGAAGAAGGCTACCGACACGTCATTGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCtt                                                                                         >  2:154675/1‑113 (MQ=255)                                                                                      ccGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGcc                                                     <  1:14599/149‑1 (MQ=255)                                                                                      ccGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGcc                                                     <  1:20895/149‑1 (MQ=255)                                                                                            aagGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCcgacga                                                                                                                  >  5:257733/1‑82 (MQ=255)                                                                                            aagGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCcgacga                                                                                                                  <  6:257733/82‑1 (MQ=255)                                                                                                      acacGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGtc                                     <  1:347081/149‑1 (MQ=255)                                                                                                        acGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGtctt                                   >  4:42901/1‑149 (MQ=255)                                                                                                          gTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGtcttct                                 >  7:253483/1‑149 (MQ=255)                                                                                                           tCATCGCGGCCCTTGAGCGTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTcttctt                                <  8:102004/149‑1 (MQ=255)                                                                                                              tCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGcc                             >  8:142004/1‑149 (MQ=255)                                                                                                                gcgGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGt                           <  8:16777/149‑1 (MQ=255)                                                                                                                 cgGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCg                            >  2:324136/1‑147 (MQ=255)                                                                                                                  ggCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAgcc                                                              >  4:84708/1‑112 (MQ=255)                                                                                                                  ggCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAgcc                                                              <  3:84708/112‑1 (MQ=255)                                                                                                                      cTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCGTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGAtct                     >  7:362657/1‑149 (MQ=255)                                                                                                                          aGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCgg                  <  5:164712/148‑1 (MQ=255)                                                                                                                           gCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGc                 <  2:385826/148‑1 (MQ=255)                                                                                                                            cTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGtc                                     >  1:34398/1‑127 (MQ=255)                                                                                                                            cTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGtc                                     <  2:34398/127‑1 (MQ=255)                                                                                                                               ggCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGCcatca            >  8:342407/1‑149 (MQ=255)                                                                                                                                gccgccAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGCcatcat           >  4:376783/1‑149 (MQ=255)                                                                                                                                 ccgccAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGCCATCATg          >  5:249854/1‑149 (MQ=255)                                                                                                                                  cgccAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAg                         <  3:155696/133‑1 (MQ=255)                                                                                                                                  cgccAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAg                         >  4:155696/1‑133 (MQ=255)                                                                                                                                      aGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCg                                        >  7:27849/1‑114 (MQ=255)                                                                                                                                      aGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCg                                        <  8:27849/114‑1 (MQ=255)                                                                                                                                      aGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGCCATCATGCCGTa     <  8:286123/149‑1 (MQ=255)                                                                                                                                            gccCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGCCATCATGCCGTacac  >  2:224964/1‑146 (MQ=255)                                                                                                                                            gccCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGCCATCATGCCGTacac  <  1:224964/146‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                           CTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGATACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGCCATCATGCCGTACAC  >  NC_002947/644495‑644779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑