Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,069,277:1	+C	5.6%	coding (331/897 nt)	PP_5323 ←	M23/M37 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,069,277	1	.	C	5.6%	288.6 / 11.1	71	coding (331/897 nt)	PP_5323	M23/M37 family peptidase
Reads supporting (aligned to +/- strand):  ref base . (36/31);  new base C (2/2);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

GCGGAACGGCCCGCCAATCCACGGGAACGCATAGCGGAAGCCCTGGGGCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCACCGTCGAAGGTTTTCGCGTT  >  NC_002947/6069131‑6069421                                                                                                                                                    |                                                                                                                                                   gcgGAACGGCCCGCCAATCCACGGGAACGCATAGCGGAAGCCCTGGGGCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGcg‑cg                                                                                                                                                  <  7:227800/149‑1 (MQ=255)  cgGAACGGCCCGCCAATCCACGGGAACGCATAGCGGAAGCCCTGGGGCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCg‑cgg                                                                                                                                                 >  4:2934/1‑149 (MQ=255)   ggAACGGCCCGCCAATCCACGGGAACGCATAGCGGAAGCCCTGGGGCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCg‑cggc                                                                                                                                                >  1:353379/1‑149 (MQ=255)     aaCGGCCCGCCAATCCACGGGAACGCATAGCGGAAGCCCTGGGGCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑cggcgg                                                                                                                                              <  6:161464/149‑1 (MQ=255)         gCCCGCCAATCCACGGGAACGCATAGCGGAAGCCCTGGGGCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGgcagc                                                                                                                                          <  8:377611/149‑1 (MQ=255)                     aCGGGAACGCATAGCGGAAGCCCTGGGGCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCGCCGGCTGCTGCCGCC‑GCGCAcc                                                                                                                              <  3:94235/149‑2 (MQ=255)                         gAACGCATAGCGGAAGCCCTGGGGCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGgcagca                                                                                                                                         <  3:33374/134‑1 (MQ=255)                         gAACGCATAGCGGAAGCCCTGGGGCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGgcagca                                                                                                                                         >  4:33374/1‑134 (MQ=255)                         gAACGCATAGCGGAAGCCCTGGGGCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATAc                                                                                                                          >  6:103861/1‑149 (MQ=255)                             gCATAGCGGAAGCCCTGGGGCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCgg                                                                                                                      >  2:389399/1‑149 (MQ=255)                              cATAGCGGAAGCCCTGGGGCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGGCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGGGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCggg                                                                                                                     <  1:303894/149‑1 (MQ=255)                                           cTGGGGCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCtt                                                                                                        <  5:103861/149‑1 (MQ=255)                                           cTGGGGCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCtt                                                                                                        >  3:122639/1‑149 (MQ=255)                                                gCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCg                                                                                                   >  7:19526/1‑149 (MQ=255)                                                gCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCg                                                                                                   >  1:30787/1‑149 (MQ=255)                                                    tttGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAACAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTtgt                                                                                                      >  3:394874/1‑142 (MQ=255)                                                    tttGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAACAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTtgt                                                                                                      <  4:394874/142‑1 (MQ=255)                                                       gCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGAc                                                                                              >  8:61516/1‑147 (MQ=255)                                                        cccGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCACCGCCTTGTGCGTTGACCAg                                                                                           <  4:298332/149‑1 (MQ=255)                                                         ccGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAgg                                                                                          <  5:252742/149‑1 (MQ=255)                                                            ggTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCGCCTGAGCACAATGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGACGCCTTGTGGGTTGACAAGGTTg                                                                                       >  4:319614/1‑149 (MQ=255)                                                            cgTCACTCAGGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTg                                                                                       <  5:101969/148‑1 (MQ=255)                                                                aCCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCa                                                                                   <  2:353379/149‑1 (MQ=255)                                                                 cccATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGc                                                                                                                   <  4:292888/116‑1 (MQ=255)                                                                 cccATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGc                                                                                                                   >  3:292888/1‑116 (MQ=255)                                                                 cccATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAg                                                                                  >  4:50786/1‑149 (MQ=255)                                                                  ccATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGc                                                                                 >  8:115229/1‑149 (MQ=255)                                                                  ccATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGc                                                                                 >  7:249499/1‑149 (MQ=255)                                                                   cATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTg                                                                                                <  2:142591/133‑1 (MQ=255)                                                                   cATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTg                                                                                                >  1:142591/1‑133 (MQ=255)                                                                   cATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGcc                                                                                <  3:50786/149‑1 (MQ=255)                                                                    agggCCTGCAGCAGGGTCGAACGGTGGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGTATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGc                                                                                 <  4:345115/145‑1 (MQ=255)                                                                    aTGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCg                                                                               >  3:95442/1‑149 (MQ=255)                                                                       gCCTGCAGGAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCaa                                                                            <  1:352739/149‑1 (MQ=255)                                                                        ccTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCaa                                                                            >  4:278573/1‑148 (MQ=255)                                                                            cagcagGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAActcta                                                                       <  4:341575/149‑1 (MQ=255)                                                                            cagcagGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAActct                                                                        <  3:2934/148‑1 (MQ=255)                                                                               cagGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCTTTTTGTCCAGCCGCAActctacct                                                                    <  7:61516/149‑1 (MQ=255)                                                                                        aCGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGca                                                         >  4:91658/1‑149 (MQ=255)                                                                                         cGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGca                                                         >  8:46674/1‑148 (MQ=255)                                                                                          ggTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTTCGTGGTGCG‑CGGCGGAAGCAGCCGGCGCAAGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCAAGc                                                                                 >  7:223526/1‑125 (MQ=255)                                                                                          ggTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGACATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGcaca                                                       <  1:389399/149‑1 (MQ=255)                                                                                              gTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCa                                                   <  4:122639/149‑1 (MQ=255)                                                                                                         gcCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGt                                        >  1:207790/1‑149 (MQ=255)                                                                                                         gcCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGt                                        <  3:278573/149‑1 (MQ=255)                                                                                                          cccGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTCGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTg                                       >  2:122257/1‑149 (MQ=255)                                                                                                                 cgccCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGaaaa                                <  2:30787/149‑1 (MQ=255)                                                                                                                 cgccCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGaaaa                                <  2:207790/149‑1 (MQ=255)                                                                                                                   cccccTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGc                              >  5:2403/1‑149 (MQ=255)                                                                                                                      ccTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCa                           >  7:351396/1‑149 (MQ=255)                                                                                                                         gAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGaaaa                                >  4:394815/1‑141 (MQ=255)                                                                                                                         gAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGaaaa                                <  3:394815/141‑1 (MQ=255)                                                                                                                         gAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGc                        >  4:43514/1‑149 (MQ=255)                                                                                                                         gAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTACACCAGGTGATCCCCCCGCAACTCTACCTC‑T‑ACCCGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGc                        >  1:132255/1‑149 (MQ=255)                                                                                                                           gCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCa                      >  5:300022/1‑149 (MQ=255)                                                                                                                            cacaCTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCg                                    <  3:266953/134‑1 (MQ=255)                                                                                                                            cacaCTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCg                                    >  4:266953/1‑134 (MQ=255)                                                                                                                             acacTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCAcc                    >  2:84313/1‑149 (MQ=255)                                                                                                                              cacTGAGCATCTGCGTGGTTGG‑GGGGGGCAGCAGGCGGCGGACGTTACGCGGGGCATGGCCGGCTTGTTGGTTGTCCAGGTTTTCCATCCGCAACTCTAACTC‑TCACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCAcc                    <  1:84313/149‑1 (MQ=255)                                                                                                                              cacTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGAAGCTCGTTTCGTGCCGAAAAGCTCACGCCAcag                   >  6:270878/1‑147 (MQ=255)                                                                                                                               acTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCACCGt                  >  4:336235/1‑149 (MQ=255)                                                                                                                                   aGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCACCGTCg                >  5:111607/1‑147 (MQ=255)                                                                                                                                   aGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCACCGTCGaa              <  7:115229/149‑1 (MQ=255)                                                                                                                                    gCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACTCCACCGTCGAAg             <  6:2403/149‑1 (MQ=255)                                                                                                                                         tGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCAc                     <  1:364946/136‑1 (MQ=255)                                                                                                                                         tGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCAc                     >  2:364946/1‑136 (MQ=255)                                                                                                                                          gtatcgtgCGCCGGCTGCTGCCGCC‑GCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCACCGTCGAAg             >  8:334783/6‑143 (MQ=255)                                                                                                                                          gtatcgtgCGCCGGCTGCTGCCGCC‑GCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCACCGTCGAAg             <  7:334783/138‑1 (MQ=255)                                                                                                                                          gCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCACCGTCGAAGGTTTTc       >  2:382976/1‑149 (MQ=255)                                                                                                                                          gCGTGGTGCG‑CGGCGGCAACAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCACCGTCGAAGGTTTTc       >  1:392607/1‑149 (MQ=255)                                                                                                                                           cGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCACCGTCGAAGGTTTTc       <  4:95442/148‑1 (MQ=255)                                                                                                                                           cGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCACCGTCGAAGGTTTTc       <  3:91658/148‑1 (MQ=255)                                                                                                                                            gtggtgCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACACCACCGTCGAAGGTTTTcgc     >  1:215042/1‑149 (MQ=255)                                                                                                                                               gtgCGCCGGCTGCTGCCGCC‑GCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCACCGTCGAAGGTTTTCGCGtt  >  6:28729/1‑149 (MQ=255)                                                                                                                                                tgCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGGCGTCCAGCCGCAACTCTACCTCAC‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCACCGTCGAAGGTTTTCGCGtt  <  1:122257/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GCGGAACGGCCCGCCAATCCACGGGAACGCATAGCGGAAGCCCTGGGGCCGTTTGCCCGGGTCACCCATGGCCTGCAGCAGGGTCGAACGGTAGTTCAGCAGCAGCCCGGGCCGCCCCCTGAGCACACTGAGCATCTGCGTGGTGCG‑CGGCGGCAGCAGCCGGCGCACGATACGCGGGGCATCGCCGCCTTGTGCGTTGACCAGGTTGTCCAGCCGCAACTCTACCTC‑T‑ACCGGCACATGCAGCTCGTTTCGTGCCGAAAAGCTCACGCCACCGTCGAAGGTTTTCGCGTT  >  NC_002947/6069131‑6069421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑