Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,866,103:1 +G 5.4% coding (608/645 nt) PP_1669 → NLP/P60 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,866,1031.G 5.4% 301.4 / 11.2 74coding (608/645 nt)PP_1669NLP/P60 family protein
Reads supporting (aligned to +/- strand):  ref base . (41/29);  new base G (2/2);  total (43/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.73e-01

TGGCCGCGGCCGGGTAAGCCATGCCGGCATCTACCTGGGTGACAACCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCAACTCTTTTTCGGCTGCA  >  NC_002947/1865960‑1866251
                                                                                                                                                 |                                                                                                                                                    
tGGCCGCGGCCGGGTAAGCCATGCCGGCATCTACCTGGGTGACAACCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTgg                                                                                                                                                 <  5:44419/149‑1 (MQ=255)
    cgcgGCCGGGTAAGCCATGCCGGCATCTACCTGGGTGACAACCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCAt                                                                                                                                             <  4:366022/149‑1 (MQ=255)
                  ccATGCCGGCATCTACCTGGGTGACAACCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAAc                                                                                                                                >  3:73716/1‑148 (MQ=255)
                        cGGCATCTACCTGGGTGACAACCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATcgcga                                                                                                                         <  4:73716/149‑2 (MQ=255)
                         ggCATCTACCTGGGTGACAACCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATcgcgcg                                                                                                                        >  3:201552/1‑149 (MQ=255)
                            aTCTACCTGGGTGACAACCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCaa                                                                                                                     >  5:97324/1‑149 (MQ=255)
                                aCCTGGGTGACAACCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATgg                                                                                                                                           >  2:345951/1‑123 (MQ=255)
                                 ccTGGGTGACAACCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAAc                                                                                                                    <  7:158970/145‑1 (MQ=255)
                                 ccTGGGTGACAACCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACt                                                                                                                   <  2:249849/146‑1 (MQ=255)
                                         aCAACCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATcgcg                                                                                                                          >  4:13675/1‑131 (MQ=255)
                                         aCAACCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATcgcg                                                                                                                          <  3:13675/131‑1 (MQ=255)
                                         aCAACCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCaaaa                                                                                                         <  1:264500/148‑1 (MQ=255)
                                         aCAACCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCaaaa                                                                                                         <  4:57181/148‑1 (MQ=255)
                                           aaCCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑G‑GCGCGCTTGGctt                                                                                                                                              <  3:196280/109‑3 (MQ=255)
                                           aaCCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑G‑GCGCGCTTGGctt                                                                                                                                              >  4:196280/1‑107 (MQ=255)
                                            aCCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAatgat                                                                                                     <  3:189601/149‑1 (MQ=255)
                                                gTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGTGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTAc                                                                                                 >  6:422522/1‑149 (MQ=255)
                                                gTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑G‑GCGCGCTTGGctt                                                                                                                                              >  8:240839/1‑102 (MQ=255)
                                                gTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑G‑GCGCGCTTGGctt                                                                                                                                              <  7:240839/104‑3 (MQ=255)
                                                     tCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTg                                                                                                                                                  >  5:270854/1‑95 (MQ=255)
                                                     tCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTg                                                                                                                                                  <  6:270854/95‑1 (MQ=255)
                                                     tCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGc                                                                                            >  3:297561/1‑149 (MQ=255)
                                                       cACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCcg                                                                                          >  2:127164/1‑149 (MQ=255)
                                                        aCTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCcgc                                                                                         >  2:123412/1‑149 (MQ=255)
                                                           ccAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCgcggc                                                                                      >  5:143822/1‑149 (MQ=255)
                                                            cagcagCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCgcggcg                                                                                     >  5:90840/1‑149 (MQ=255)
                                                            cagcagCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCgcgg                                                                                       <  4:201552/147‑1 (MQ=255)
                                                               cagcCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGTCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGt                                                                                   >  2:45020/1‑148 (MQ=255)
                                                               cagcCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGt                                                                                   >  2:305141/1‑148 (MQ=255)
                                                                      cgcAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCttt                                                                           <  7:227951/149‑1 (MQ=255)
                                                                      cgcAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCttt                                                                           >  3:365584/1‑149 (MQ=255)
                                                                       gcAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTg                                                                          <  1:305141/149‑1 (MQ=255)
                                                                             gtggtgTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGaaaaa                                                                     >  6:167404/1‑148 (MQ=255)
                                                                              tggtgTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGaaaaaa                                                                    >  4:329275/1‑148 (MQ=255)
                                                                               ggtgTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGaaaaaaaa                                                                  <  7:79864/149‑1 (MQ=255)
                                                                                   tgCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTc                                                              <  6:90840/149‑1 (MQ=255)
                                                                                    gcgcATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCa                                                             >  8:21605/1‑149 (MQ=255)
                                                                                        aTCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCAGCGGTGATGCTTTGAAAAAAAAGCTCAccgc                                                         >  6:78447/1‑149 (MQ=255)
                                                                                           gACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCAccgccg                                                       <  5:422522/148‑1 (MQ=255)
                                                                                           gACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGt                                                      >  8:47433/1‑149 (MQ=255)
                                                                                             cAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTgc                                                    <  3:329275/149‑1 (MQ=255)
                                                                                             cAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTgc                                                    <  5:167404/149‑1 (MQ=255)
                                                                                                ccTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCaaa                                                                                                          >  3:189725/1‑92 (MQ=255)
                                                                                                ccTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCaaa                                                                                                          <  4:189725/92‑1 (MQ=255)
                                                                                                ccTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCa                                                 >  7:41761/1‑149 (MQ=255)
                                                                                                  tCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCAtt                                               >  8:272899/1‑149 (MQ=255)
                                                                                                          cGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGa                                         >  5:217666/1‑147 (MQ=255)
                                                                                                           gCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCAtt                                               >  1:379561/1‑140 (MQ=255)
                                                                                                           gCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCAtt                                               <  2:379561/140‑1 (MQ=255)
                                                                                                             tACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCg                                    >  7:135232/1‑149 (MQ=255)
                                                                                                                tGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGtc                                  >  4:212075/1‑148 (MQ=255)
                                                                                                                  gAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGtcta                                <  7:21605/148‑1 (MQ=255)
                                                                                                                     cAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGtctaatc                             >  5:87533/1‑148 (MQ=255)
                                                                                                                     cAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGtctaatc                             >  4:362749/1‑148 (MQ=255)
                                                                                                                      aaGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGtctaatct                            >  3:402413/1‑148 (MQ=255)
                                                                                                                           cTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGaa                                        >  3:255564/1‑131 (MQ=255)
                                                                                                                           cTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGaa                                        <  4:255564/131‑1 (MQ=255)
                                                                                                                           cTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGc                                     <  3:275848/134‑1 (MQ=255)
                                                                                                                           cTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGc                                     >  4:275848/1‑134 (MQ=255)
                                                                                                                           cTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATtc                      >  3:67007/1‑149 (MQ=255)
                                                                                                                            ttCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATtc                      >  2:66265/1‑148 (MQ=255)
                                                                                                                            ttCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATtc                      >  8:31876/1‑148 (MQ=255)
                                                                                                                              cATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATtct                     <  6:87533/147‑1 (MQ=255)
                                                                                                                               aTTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCaa                  <  8:135232/149‑1 (MQ=255)
                                                                                                                               aTTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCaa                  <  4:67007/149‑1 (MQ=255)
                                                                                                                                 catggCCAAGGCGCG‑‑CTTGGCTATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCAAct                >  7:313869/5‑149 (MQ=255)
                                                                                                                                     gCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCTGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCAACTCttt            <  5:78447/149‑1 (MQ=255)
                                                                                                                                     gCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCAACTCttt            >  2:219691/1‑149 (MQ=255)
                                                                                                                                     gCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGAACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCAACTCttt            >  5:89633/1‑149 (MQ=255)
                                                                                                                                       cAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCAACTCttttt          >  6:353540/1‑149 (MQ=255)
                                                                                                                                        aa‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGGAATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCAACTCTTTTTc         >  1:291505/1‑149 (MQ=255)
                                                                                                                                        aa‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGTCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCAACTCTTTTTc         <  2:5924/149‑1 (MQ=255)
                                                                                                                                        aa‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCAACTCTTTTTc         >  4:260987/1‑149 (MQ=255)
                                                                                                                                        aa‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCAACTCTTTTTc         <  6:143822/149‑1 (MQ=255)
                                                                                                                                           gcgcgc‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCAACTCTTTTTCgg       <  1:123412/149‑1 (MQ=255)
                                                                                                                                             gcgc‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAAtt                       >  2:99393/1‑131 (MQ=255)
                                                                                                                                             gcgc‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAAtt                       <  1:99393/131‑1 (MQ=255)
                                                                                                                                             gcgc‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCAACTCTTTTTCGGCt     >  7:164340/1‑149 (MQ=255)
                                                                                                                                             gcgc‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCAACTCTTTTTCGGCt     >  7:379422/1‑149 (MQ=255)
                                                                                                                                                c‑cTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCAACTCTTTTTCGGCTgca  <  6:217666/149‑1 (MQ=255)
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TGGCCGCGGCCGGGTAAGCCATGCCGGCATCTACCTGGGTGACAACCAGTTCATCCACTCCAGCAGCCGCCGCAGTGGTGGTGTGCGCATCGACAGCCTCGGTGATCGCTACTGGAGCAAGACCTTCATTGAAGCCAA‑GCGCGC‑CTTGGCCATGGCGCCGACCAACATCGCGCGCAACTGATATCAAAATGATGTACCCTGCCGCGGCGGTGATGCTTTGAAAAAAAAGCTCACCGCCGTGCGCATTTACAGAAAGCGTCTAATCTAAATTCTCAACTCTTTTTCGGCTGCA  >  NC_002947/1865960‑1866251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑