| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,845,703:1 | +G | 100% | intergenic (‑70/+28) | PP_3394 ← / ← PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,845,703 | 1 | . | G | 97.3% | 122.8 / ‑3.8 | 39 | intergenic (‑70/+28) | PP_3394/PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (1/0); major base G (21/16); minor base T (1/0); total (23/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑GG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCC > NC_002947/3845558‑3845845 | ctgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑ggg < 1:205772/149‑2 (MQ=255) tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATc > 7:9742/1‑149 (MQ=255) tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATc > 7:131226/1‑149 (MQ=255) gCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCAt > 7:357863/1‑149 (MQ=255) aTCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTc < 8:275993/148‑1 (MQ=255) tCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGtggc > 5:45471/1‑146 (MQ=255) ttGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCAc > 5:396327/1‑149 (MQ=255) gCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGCCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGa > 1:235079/1‑149 (MQ=255) ggCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGCCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATgg < 2:235079/149‑1 (MQ=255) gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGt < 8:251635/136‑1 (MQ=255) gCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGt > 7:251635/1‑136 (MQ=255) tctGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGAt > 6:403242/1‑148 (MQ=255) cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTGGCTGACGATGGATCa < 6:45471/146‑1 (MQ=255) cAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATACCTCGTCAGCCCCGATGGCTCAGcc > 5:61782/1‑149 (MQ=255) cgcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCACGCGCCCTGc > 8:34190/1‑149 (MQ=255) gcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGAATAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGcc < 2:67466/149‑1 (MQ=255) aCTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGt < 5:152865/149‑1 (MQ=255) aCTTCATTCATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACTATGGATCAGCCCGCGCCCTGCCAGGCCGGt < 6:413239/149‑1 (MQ=255) ttCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTg > 5:163400/1‑148 (MQ=255) attgttattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑GGCCTGATCCAAAGTCAGCCACGATTGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgcag > 3:224965/1‑148 (MQ=255) tattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCcg < 3:25002/118‑1 (MQ=255) tattgttGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCcg > 4:25002/1‑118 (MQ=255) tCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGa > 5:388953/1‑99 (MQ=255) tCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGa < 6:388953/99‑1 (MQ=255) cgGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGgc < 5:100660/149‑1 (MQ=255) cgGTCTCTCTGTCTGAAGCGTTGCGCAAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGAGGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGgc < 5:201155/149‑1 (MQ=255) tctctGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCTGG‑CTGATCAATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgcc > 5:296668/1‑147 (MQ=255) aGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCAACATGGATCAGCCCCCGCCCTGCCAGGc > 3:189052/1‑101 (MQ=255) ttGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGc > 7:10832/1‑125 (MQ=255) agagCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTcgc < 6:163400/149‑1 (MQ=255) gCTGGATGACGGGCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCAGACCGCGAGAGCGTGTGCCGGTCgcgcc > 4:260685/1‑148 (MQ=255) gggCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGAc > 6:124530/1‑149 (MQ=255) gggCAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCAAGGCCGGTGCACGCAGCGACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGAc > 3:261802/1‑149 (MQ=255) cAGAAAAGACGTGTTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGaa > 2:256717/1‑149 (MQ=255) aaaacccGTTTTTCGCGGG‑CTGATCCATGGTCAGCCACGATGGACCAGCCCGCGCCCTGCCAGGCCGGTGCACGCATCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGATCGTGTGCCGGTCGCGCATGCAGATGACGAATTc < 7:34190/143‑1 (MQ=255) gtgtTTCGCGGG‑CTGATCCATCGTCCGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagccga < 8:90102/100‑1 (MQ=255) gtgtTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgccgagccga > 7:90102/1‑100 (MQ=255) gtTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTc > 6:414632/1‑80 (MQ=255) gtTTCGCGGG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTc < 5:414632/80‑1 (MQ=255) tttCGCGGG‑CTGATCCTTCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGcc < 6:296668/149‑1 (MQ=255) | CTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTTCGC‑GG‑CTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCAAGCGCC > NC_002947/3845558‑3845845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |