Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_002947	4,980,585	+GGC	100%	intergenic (‑24/+44)	PP_4387 ← / ← flgE	hypothetical protein/flagellar hook protein FlgE

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 4980585	2 (0.030)	44 (0.700) +GGC	34/248	NT	95.8%	intergenic (‑24/+44)	PP_4387/flgE	hypothetical protein/flagellar hook protein FlgE
	?	NC_002947	4980586 =	2 (0.030)					intergenic (‑25/+43)	PP_4387/flgE	hypothetical protein/flagellar hook protein FlgE

CGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/4980442‑4980585 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGC  >  NC_002947/4980586‑4980732                                                                                                                                                  |||                                                                                                                                                    CGAACACCGGCAGCGGCCGGTCACCAGGCTTTCCCATTGAAACACCGGGGCGGGGCCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCG                                                                                                                                                   <  3:387365/149‑1      ACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTG                                                                                                                                              <  5:379161/149‑1             cCGGCCGGGGACCAGTCTGTGCCATTGATAGAACGGGGCGTTGGCAGGGGGGGCAGGCAGAACCAGCACCAGCATGGGGACCTAGTCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCC                                                                                                                                        <  8:378123/147‑1             GCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCC                                                                                                                                        <  1:145545/148‑1                      GACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAA                                                                                                                              <  4:386021/149‑1                       ACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAA                                                                                                                              >  5:332672/1‑148                             cttgcCCATTGATCCAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCATCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAA                                                                                                                       <  3:248673/144‑1                                  CCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCA                                                                                                                   >  7:214072/1‑148                                    ATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATA                                                                                                                <  3:142332/149‑1                                     TTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATA                                                                                                                >  4:275993/1‑148                                        ATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACA                                                                                                                            <  4:391513/133‑1                                        ATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACA                                                                                                                            >  3:391513/1‑133                                               GGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGG                                                                                                     <  5:329354/149‑1                                                    GGGGGCAGCGGGGGCAGGTAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATC                                                                                                <  6:162521/149‑1                                                     GGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTGTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCT                                                                                               <  8:214072/149‑1                                                      GGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCT                                                                                               >  6:362003/1‑148                                                        GCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTG                                                                                              >  1:297072/1‑148                                                        GCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAA                                                                                            >  3:359548/1‑149                                                                   AGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGattgta                                                                                 <  4:251517/149‑7                                                                      CAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACCCCAAAGCCAATACAATAATAAGGTCATCTGAATGATGGTCTGCAT                                                                               >  2:347315/1‑148                                                                            CAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCGTTGCCGCCCCAATTACAAACACCAAAGCCAATAGAATCATCAGGTCATCTGAATGATGGTCGGCATGATGGTt                                                                        >  6:424453/1‑148                                                                                ACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTT                                                                    >  2:74896/1‑149                                                                                ACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCATTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTAATAGGAATGATGGTCTGCATGATGGTGCTT                                                                     >  3:113132/1‑148                                                                                  CAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTT                                                                    >  3:126182/1‑147                                                                                     CAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTG                                                               >  8:98277/1‑149                                                                                         GGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAA                                                           <  4:126182/149‑1                                                                                         GGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAA                                                           <  3:275993/149‑1                                                                                         GGGAACAAGGCCA‑GTAACGACAACCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAAACAACAGGTAATCTGAATGATGGTCGGCATGATGGTGCATTCAATTGAA                                                            >  7:63082/1‑148                                                                                          GGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAAT                                                          <  2:297072/149‑1                                                                                          GGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAAT                                                          <  4:359548/149‑1                                                                                               AAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCT                                                     >  1:102961/1‑149                                                                                                AGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATC                                                                                                >  5:150020/1‑105                                                                                                AGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATC                                                                                                <  6:150020/105‑1                                                                                                        TAACGACTGCCTCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTG                                             <  1:347315/149‑1                                                                                                              CAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGG                                       <  5:362003/149‑1                                                                                                               AGCCGCTTCATTCGCTTGAACCTTCACATTCTTGGGTCCGCTTTGCGTCCCCAATTACTAACACCAAAGCCAATACAATCATCATGTCAGCTGAATGATTGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGg                                      <  8:291031/149‑2                                                                                                                 CCGCTTCATTCGCGTGAAACTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATATGAATGATGGTCTGCATTATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTA                                     <  2:144755/148‑1                                                                                                                    CTTCATTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAAAACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTG                                 >  6:263493/1‑149                                                                                                                         TTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTG                            >  6:4570/1‑149                                                                                                                         TTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTG                                                               <  7:315037/114‑1                                                                                                                         TTCGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTG                                                               >  8:315037/1‑114                                                                                                                           CGCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGC                           <  1:74896/148‑1                                                                                                                            GCGTGAACCTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGC                         >  6:108435/1‑149                                                                                                                                    CTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAACGCCAATACAATCATCACGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGGTGCTCTGCGCCGTGAT                   >  8:95819/1‑147                                                                                                                                    CTTCACATTCTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGgt                   >  4:51933/1‑145                                                                                                                                           taaTTGGGGCCGCAAAGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTAC          >  2:126524/4‑149                                                                                                                                             CTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACC         <  5:4570/148‑1                                                                                                                                             CTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTG                                        <  8:393102/117‑1                                                                                                                                             CTTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCGGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTG                                        >  7:393102/1‑117                                                                                                                                              TTGGGGCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTAC          >  3:444/1‑146                                                                                                                                                   GCCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGC  >  1:365918/1‑149                                                                                                                                                  |||                                                                                                                                                    CGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCA‑GTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/4980442‑4980585 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGC  >  NC_002947/4980586‑4980732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.