Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,301,579 G→C 6.4% intergenic (+69/‑1231) PP_3774 → / → PP_5627 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,301,5790GC 6.4% 199.6 / 3.0 62intergenic (+69/‑1231)PP_3774/PP_5627hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (29/29);  new base C (2/2);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

GGTCGACTACACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATCTCTGCCTGC  >  NC_002947/4301432‑4301725
                                                                                                                                                   |                                                                                                                                                  
ggTCGACTACACCGACGCAACCCCCGCCGAGAACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCgggg                                                                                                                                                   >  1:250961/1‑149 (MQ=255)
          acCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCAACGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCAAAAGGCGACGCATGAATGAAAAAAACCCACCGCAgggatgggataa                                                                                                                                          >  8:257548/1‑144 (MQ=255)
            cGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTAt                                                                                                                                       <  7:285534/149‑1 (MQ=255)
                gCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGgggctgggc                                                                                                                                             >  5:418057/1‑139 (MQ=255)
                gCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGgggctgggc                                                                                                                                             <  6:418057/139‑1 (MQ=255)
                gCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCCCCGCGCTGGGCttt                                                                                                                                          <  1:207137/142‑2 (MQ=255)
                gCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCCCCGCGCTGGGCttt                                                                                                                                          >  2:207137/1‑141 (MQ=255)
                  aaCCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTg                                                                                                                                 >  7:71597/1‑149 (MQ=255)
                    cccccGCCTAGGACTGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTggg                                                                                                                               <  7:257548/149‑1 (MQ=255)
                     ccccGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGc                                                                                                                              <  3:128106/149‑1 (MQ=255)
                         gccgAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGaa                                                                                                                          <  5:411157/149‑1 (MQ=255)
                              ggACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTa                                                                                                                     <  4:222018/149‑1 (MQ=255)
                                  ggCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTc                                                                                                                 >  1:408230/1‑149 (MQ=255)
                                    cTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCCCCGCGCTGGGCttt                                                                                                                                          <  6:214247/122‑2 (MQ=255)
                                    cTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCCCCGCGCTGGGCttt                                                                                                                                          >  5:214247/1‑121 (MQ=255)
                                            gCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCtt                                                                                                       >  8:262771/1‑149 (MQ=255)
                                            gCATCATAAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCt                                                                                                        <  2:250961/148‑1 (MQ=255)
                                             catcatCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCtt                                                                                                       >  2:295428/1‑148 (MQ=255)
                                             catcatCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCtt                                                                                                       >  4:105602/1‑148 (MQ=255)
                                              atcatcAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTa                                                                                                     >  3:154585/1‑149 (MQ=255)
                                                   caGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTAt                                                                                                                                       >  6:10801/1‑110 (MQ=255)
                                                   caGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTAt                                                                                                                                       <  5:10801/110‑1 (MQ=255)
                                                    aGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACt                                                                                               >  7:355595/1‑149 (MQ=255)
                                                     gCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTg                                                                                              >  5:170071/1‑149 (MQ=255)
                                                           tctcGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGgggctg                                                                                                                                                >  1:138634/1‑93 (MQ=255)
                                                           tctcGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGgggctg                                                                                                                                                <  2:138634/93‑1 (MQ=255)
                                                           tctcGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTg                                                                                         >  5:207525/1‑148 (MQ=255)
                                                             tcGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGaa                                                                                      >  6:59093/1‑149 (MQ=255)
                                                               gCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAg                                                                                     <  3:105602/148‑1 (MQ=255)
                                                                   atccatcGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACt                                                                                >  2:81248/1‑149 (MQ=255)
                                                                    tccatcGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTc                                                                               <  8:71597/149‑1 (MQ=255)
                                                                      catcGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTc                                                                               >  5:272180/1‑147 (MQ=255)
                                                                       atcGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTacaca                                                                                                 <  3:726/128‑1 (MQ=255)
                                                                       atcGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTacaca                                                                                                 >  4:726/1‑128 (MQ=255)
                                                                              aaCGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTAc                                                                                           >  1:153858/1‑127 (MQ=255)
                                                                              aaCGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTAc                                                                                           <  2:153858/127‑1 (MQ=255)
                                                                                 gTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTtg                                                                  <  2:337191/149‑1 (MQ=255)
                                                                                  tCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTTTACTGGAAGTTACTCCAGTGGGCGCTtgt                                                                 <  8:355595/149‑1 (MQ=255)
                                                                                  tCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTtgt                                                                 <  4:253879/149‑1 (MQ=255)
                                                                                    gATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGa                                                               <  2:408230/149‑1 (MQ=255)
                                                                                        cccAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAAc                                                           <  7:262771/149‑1 (MQ=255)
                                                                                         ccAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAAc                                                           >  5:139826/1‑148 (MQ=255)
                                                                                         ccAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACt                                                          >  7:131149/1‑149 (MQ=255)
                                                                                            gCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCCCTTGAGATAACtat                                                        >  4:133798/1‑148 (MQ=255)
                                                                                             cccTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATaa                                                      <  6:207525/149‑1 (MQ=255)
                                                                                                  cTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTc                                                 <  1:81248/149‑1 (MQ=255)
                                                                                                  cTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTc                                                 <  6:170071/149‑1 (MQ=255)
                                                                                                   tATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCg                                                >  8:23723/1‑149 (MQ=255)
                                                                                                        aaaCCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCAtt                                           >  7:226107/1‑149 (MQ=255)
                                                                                                          aCCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTgg                                         <  6:272180/149‑1 (MQ=255)
                                                                                                             ccTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATg                                      >  5:370377/1‑149 (MQ=255)
                                                                                                              cTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTacac                                                                                                  >  4:330819/1‑88 (MQ=255)
                                                                                                              cTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTacac                                                                                                  <  3:330819/88‑1 (MQ=255)
                                                                                                                 aGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGACGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGccc                                   <  8:226107/148‑1 (MQ=255)
                                                                                                                      gTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATg                             >  7:381598/1‑149 (MQ=255)
                                                                                                                       tCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATgg                            <  8:381598/149‑1 (MQ=255)
                                                                                                                         gCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGAt                              <  4:55549/145‑1 (MQ=255)
                                                                                                                         gCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGAt                              >  3:55549/1‑145 (MQ=255)
                                                                                                                          cATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTc                         <  5:59093/149‑1 (MQ=255)
                                                                                                                                tGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCcgc                                 <  3:230617/135‑1 (MQ=255)
                                                                                                                                tGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCcgc                                 >  4:230617/1‑135 (MQ=255)
                                                                                                                                   aaaaaGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGcc                >  2:400468/1‑149 (MQ=255)
                                                                                                                                    aaaaGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCAGGCGGGCCt               >  4:13835/1‑149 (MQ=255)
                                                                                                                                      aaGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGa             >  1:171387/1‑149 (MQ=255)
                                                                                                                                          ccAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGa                               <  1:68595/127‑1 (MQ=255)
                                                                                                                                          ccAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGa                               >  2:68595/1‑127 (MQ=255)
                                                                                                                                             gcgcGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATCTctgc      <  7:173016/149‑1 (MQ=255)
                                                                                                                                               gcgGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATCTctgcct    <  1:295428/149‑1 (MQ=255)
                                                                                                                                                 ggggCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATCTctgcctgc  <  4:154585/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTCGACTACACCGACGCAACCCCCGCCGAGGACGGCTCGATCTGCATCATCAGCGAGCTCTCGCTCATCCATCGCTGAACGTCGATGCCCAGCCCTGCTATCAAAACCCCTCAGGGCGTCGCATGAATGCAAAAAGCCCAGCGCGGGGCTGGGCTTCTATCAGCTGGGCTGAACAGTACTTCCGCCAGCCTTTACACACTGTACTGGAAGTTACTCCAGTGGGCGCTTGTGATAACTATAACGCTCGGCATTGGATGCCCGCGATGGGTCCGGCGGGCCTGATCTCTGCCTGC  >  NC_002947/4301432‑4301725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑