Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 445,107 T→G 7.9% intergenic (+80/‑208) PP_0367 → / → PP_0368 hypothetical protein/acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947445,1070TG 7.9% 152.1 / 5.3 51intergenic (+80/‑208)PP_0367/PP_0368hypothetical protein/acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (25/21);  new base G (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

GCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAA  >  NC_002947/444961‑445251
                                                                                                                                                  |                                                                                                                                                
gCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAAtttt                                                                                                                                                >  4:29504/1‑149 (MQ=255)
    ggCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGt                                                                                                                                              <  2:144513/147‑1 (MQ=255)
    ggCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCt                                                                                                                                            <  3:29504/149‑1 (MQ=255)
    ggCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCCCGAATTCGTCCGATCAGACAAAAATTTTGtgt                                                                                                                                            >  7:27770/1‑147 (MQ=255)
            ggTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGc                                                                                                                                    <  5:194308/149‑1 (MQ=255)
                      gCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCgg                                                                                                                          <  6:83789/149‑1 (MQ=18)
                           cgacgaAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGTATGCGTCCGATCAGACAACAATTTTGTCTGGt                                                                                                                                         >  8:25324/1‑129 (MQ=255)
                           cgacgaAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGTATGCGTCCGATCAGACAACAATTTTGTCTGGt                                                                                                                                         <  7:25324/129‑1 (MQ=255)
                             acgaAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGGATTCCCGCGAATGCGGCCGAAGAGCCAAAAAATTTTTATGGTGGGGGCTCGTAGCGGGGCAGcc                                                                                                                   >  5:226943/1‑149 (MQ=255)
                                     cTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCgg                                                                                                                          >  2:283880/1‑134 (MQ=17)
                                     cTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCgg                                                                                                                          <  1:283880/134‑1 (MQ=17)
                                             gATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGCATGCGTCCGATCAGACAACAATTTTGTGTGGTCGGGCCTCTTCGCGGGCAAGCCCGCGCCTAAAAggggt                                                                                                   >  3:322540/1‑147 (MQ=255)
                                               tcatcGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTTCCCGCGAATGCGTCCGATAAGACAACAATTTTTTCTGGTCGGGCCTCTTAGCGGGCAATCCCGCTCCGACAACGGTTtg                                                                                                 >  6:101027/1‑149 (MQ=255)
                                                     acacCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGTTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGtggcat                                                                                           >  5:88123/1‑149 (MQ=255)
                                                     acacCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCt                                                                                                                                            <  2:342555/100‑1 (MQ=255)
                                                                tGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAAtttt                                                                                                                                                >  2:78828/1‑85 (MQ=255)
                                                                   aTACTTGCGTCATCCCTTATTTCTGCGCCTGCCTTGTTGGTGGGGGCTTGCCGGCTATTGCGTCCTATCACAAAAAATTTTTGTCTGGTCGGGCCTCTTCGCGGGAAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGAttt                                                                             <  2:341892/149‑1 (MQ=255)
                                                                       ttGCGTCATCCACTATTTCAGCGCCTGCCTTGTAGGAGCGGGCATGCCCGCGAATGAGTCCCATAACACAACAAAATTTTCTGGTGGGGCCTCGTACCGGGCAAGCCAGGGCCTACAAGCGTTTGAGGCATTGCATGCCAGCTTTggc                                                                          >  2:263719/1‑146 (MQ=255)
                                                                              aTCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGTTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCg                                                                  <  6:88123/149‑1 (MQ=255)
                                                                                ccccTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTTCCCGCGAATTCGTCCCATAAGACAACAATTTTTTATGGTCGGGCCACTTAGCGGGCCAGCCCCCTCCGAAAAGGGTTTTTGGCATGGCAAGTCTGATTTTGCATTTTTAGTg                                                                >  2:32186/1‑149 (MQ=255)
                                                                                        tCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTcg                                                                                                                             >  4:278652/1‑80 (MQ=255)
                                                                                        tCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTcg                                                                                                                             <  3:278652/80‑1 (MQ=255)
                                                                                             ccctgcctTGTTGCTTCGGGCTTGCCCGCGAATGCGTCCGATCATCAAACAATTTTGGCTGGTCGGGACTCTTCGCGGGCATGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCa                                                    <  1:263719/147‑1 (MQ=255)
                                                                                                           cgCGGGCTGCCCCGCTAAGGCGTCCGATCCCACAAAAATTTTGTCTGGCGGGGCCTCTCCGCGGGAAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCtt                                     <  6:226943/148‑1 (MQ=255)
                                                                                                             cGGGTTTGCCCGCTAATGCGGCCGATCACAAAACAATTTTGTCGGGGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGa                                   <  8:27770/149‑1 (MQ=255)
                                                                                                                    gCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGt                                             >  6:347114/1‑132 (MQ=255)
                                                                                                                    gCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGt                                             <  5:347114/132‑1 (MQ=255)
                                                                                                                     cccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCTTAAATGTCACCATAGTCCATCCTTGACAGAAAGc                           >  2:174557/1‑149 (MQ=255)
                                                                                                                     cccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAg                            <  1:47634/148‑1 (MQ=255)
                                                                                                                     cccGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGc                           >  5:39090/1‑149 (MQ=18)
                                                                                                                      ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCt                                      >  6:327631/1‑137 (MQ=255)
                                                                                                                      ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCt                                      <  5:327631/137‑1 (MQ=255)
                                                                                                                      ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGa                                   <  7:350400/140‑1 (MQ=255)
                                                                                                                      ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGa                                   >  8:350400/1‑140 (MQ=255)
                                                                                                                      ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt                          >  1:229380/1‑149 (MQ=255)
                                                                                                                      ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt                          >  5:134588/1‑149 (MQ=255)
                                                                                                                      ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt                          >  4:21296/1‑149 (MQ=255)
                                                                                                                      ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt                          >  4:178987/1‑149 (MQ=255)
                                                                                                                      ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt                          >  5:275895/1‑149 (MQ=255)
                                                                                                                      ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTACTACAAGGGTTTGTGGCATGGCATGACTGATTTGCAATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAgaa                          >  4:230645/1‑147 (MQ=255)
                                                                                                                        gctaaTGCGTCCGATCAGACAACAATTTTGTCTGGGCGGGCCTATTCGCGGGAAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGa                        <  1:221988/146‑1 (MQ=255)
                                                                                                                        gcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCa                                          <  5:423140/131‑1 (MQ=255)
                                                                                                                        gcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCa                                          >  6:423140/1‑131 (MQ=255)
                                                                                                                        gcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt                          >  2:106113/1‑147 (MQ=255)
                                                                                                                                       cacacaaaaTTTTTGTTTGGCCGGGCCTCTCCGGGGGAATGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGttt         <  2:365036/146‑1 (MQ=255)
                                                                                                                                         gACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTc        >  1:403752/1‑148 (MQ=255)
                                                                                                                                           caacaaTTTTTTCTGTTCGGGCCTCTTCGCGTGCAAGGCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCaaa     <  3:230645/149‑1 (MQ=255)
                                                                                                                                            aacaaTTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCaaa     >  2:27288/1‑148 (MQ=255)
                                                                                                                                            aacaaTTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCaaa     >  2:33581/1‑148 (MQ=255)
                                                                                                                                            aacaaTTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGAATTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCaaa     >  1:101088/1‑148 (MQ=255)
                                                                                                                                              caaTTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAggg                                                                                                     >  6:379266/1‑50 (MQ=255)
                                                                                                                                              caaTTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAggg                                                                                                     <  5:379266/50‑1 (MQ=255)
                                                                                                                                              caaTTTTGTCTGGTCGGGCCGCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAaacaa  <  5:101027/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAA  >  NC_002947/444961‑445251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑