Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,503,789 C→T 14.4% intergenic (+38/‑60) xanP → / → tusD xanthine permease/sulfur transfer protein complex subunit TusD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,503,7890CT14.4% 94.9 / 7.7 44intergenic (+38/‑60)xanP/tusDxanthine permease/sulfur transfer protein complex subunit TusD
Reads supporting (aligned to +/- strand):  ref base C (15/21);  new base T (4/2);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 3.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.68e-01

GCGCACATGCCGGCGGCGATTCGCAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGCCGGCGG  >  NC_002947/4503641‑4503937
                                                                                                                                                    |                                                                                                                                                      
gcgcACATGCCGGCGGCGATTCGCAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGc                                                                                                                                                        >  7:337797/1‑149 (MQ=255)
  tctcaTGCCGGCGGCGATTCTCAAGGTGCTGGATGCGGGGGTGGCTTCCGGGGGGTTTTGTGCGCTGGTGCTGATCTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGcac                                                                                                                                                      <  4:60014/146‑1 (MQ=255)
    acaTGCCGGCGGCGATTCGCAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGcac                                                                                                                                                      >  6:398643/1‑147 (MQ=255)
         ccGGCGGCGAGTCGCAATGTGCTGGAGGCGGGTGTTGCTACCGGGGTGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGATGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAgcgg                                                                                                                                                <  4:238959/148‑1 (MQ=255)
            gcggcgATTCGCAATGTGCTGGAGGCGGGTGGGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGTGCAAgcgggcgc                                                                                                                                            <  7:388297/149‑1 (MQ=255)
                      gCAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCttttt                                                                                                                                   <  5:176211/148‑1 (MQ=255)
                       cAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCCCGTGGACAAGCGGGGGGGGGGttttttt                                                                                                                                 >  5:39544/1‑148 (MQ=255)
                        actgtgCTGGAGTCGGGGGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGc                                                                                                                                <  1:371299/147‑1 (MQ=255)
                              cTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTAt                                                                                                                           >  7:283372/1‑148 (MQ=255)
                               tGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGATTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCa                                                                                                                         <  4:6435/149‑1 (MQ=255)
                                ggtgtggggTGTGGCTACGGGGGGTTTTTTTGCGGTGTTGCTGAACTTTTTTTTGCCGGTGGTAAAGTATCTGGTTTTAACTTTTAGACTGAATTATATTAGCCCGCGCCCGCTTGAACACGGGGGGGCGGGCTTTTTTGCTTTATCAt                                                                                                                        <  8:132670/143‑1 (MQ=255)
                                  aGTCGGGTTTGTCTACCGGGGGGATTTGTGCGCTTGTGCTGAAGTGGTTCTTGCCGGAGGGCAAGGAGCAGTCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGATTGCACAAGCTGTCGCTGGCTTTTTTGCTTTATCATg                                                                                                                       <  8:232520/148‑1 (MQ=255)
                                       ggTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGTGCGCGGGCTTTTTTTCTTTATAATTTCAcc                                                                                                                  >  4:110593/1‑148 (MQ=255)
                                              cTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCttt                                                                                                          <  3:331696/149‑1 (MQ=255)
                                                 ccGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTg                                                                                                         <  8:337797/147‑1 (MQ=255)
                                                 ccGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCa                                                                                                       >  3:298687/1‑149 (MQ=255)
                                                            tgtgCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGcc                                                                                             <  5:398643/148‑1 (MQ=255)
                                                                     gTGCTTAACTGGTTCTTGACGGATGGCAAGGATCAGGCTTGAACCTTGATAATGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTc                                                                                    <  3:110593/148‑1 (MQ=255)
                                                                          gAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATc                                                                              >  5:394709/1‑149 (MQ=255)
                                                                             cTGGTTCTTGCCGGAGGGCAAGGAGCAGTCTTGAACGTTGATACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTg                                                                                                         <  1:24395/119‑1 (MQ=255)
                                                                             cTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCCCTTGCACAAGCCGGCGCCGGCTTTTTTGCTTTATCAAGGCACCATAACTTTg                                                                                                         >  2:24395/1‑119 (MQ=255)
                                                                                gTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGgtg                                                                      >  2:98023/1‑149 (MQ=255)
                                                                                 ttCTTGCCGGAGGGCAAGGAGCAGGCTTTAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATcg‑‑cg                                                                         <  5:50963/145‑1 (MQ=255)
                                                                                   cTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTtc                                                                   >  7:301508/1‑149 (MQ=255)
                                                                                    ttGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGtt                                                                    <  3:304149/147‑1 (MQ=255)
                                                                                    ttGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGtt                                                                    >  4:304149/1‑147 (MQ=255)
                                                                                         ggAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCg                                                             <  8:283372/149‑1 (MQ=255)
                                                                                          gAGTGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCGCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCgg                                                            <  7:300890/149‑1 (MQ=255)
                                                                                          gAGGGCAAGGAGCAGGCTTGAACCTTGAGCCTGAATGACAGTAGCCCGCGCCCGCGTGCACCACCGCGCGCGGGCGTTTTTGCTTTAACCTGGACCCATTACTTTGCAAGAGTTAGCCAAGAAATTAGCGATCG‑‑AGTTGGTAGCCCCgg                                                            >  6:102319/1‑149 (MQ=255)
                                                                                                  ggAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTAGCTATCG‑‑CGGTGTTCTCCCCGGCCCATgtg                                                    >  2:121891/1‑147 (MQ=255)
                                                                                                       aGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCT‑CCCGGCCCATGCGCCctct                                              <  4:298687/149‑1 (MQ=255)
                                                                                                           ttGAACCTTGAGACTGAATGAGAGTAGCCCGCCCCCCCATGCACAAGCGGGCGCGGGCTTTTTTGCATTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATAG‑‑AGGTGTTCTCCCCGGCCCATGCGCCCACATcg                                           >  6:180514/1‑149 (MQ=255)
                                                                                                             gAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCGGCCCATGCGCCCTCTTcg                                           <  1:98023/147‑1 (MQ=255)
                                                                                                             gAACCTTGAGACGTAATGAGCGTCGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCGGCCCATGCGCCCTCTTcgc                                          <  5:102319/148‑1 (MQ=255)
                                                                                                                  ttGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGAcgcg                                    >  1:303408/1‑149 (MQ=255)
                                                                                                                    gagaCTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGAcgcg                                    <  5:180514/147‑1 (MQ=255)
                                                                                                                                gagTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGcc                      <  6:394709/149‑1 (MQ=255)
                                                                                                                                 agTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTTTCATGGCACCATTCCTTTGCATGAGTTTGCCATGAAATTAGCTATAG‑‑CGGTGTTGTCCCCCGCCCAAGCGCCCGCTTAGCGACGCGCCCGGCGCGTAGCCg                     >  8:120261/1‑149 (MQ=255)
                                                                                                                                      cccgcgcccgcTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCT‑‑CGGTGTTCTCCCCGGCCCATGCCCCCTCTTCGCCACGCGCCCTGCGCTTCGCCGAGCCg                >  6:127225/1‑149 (MQ=255)
                                                                                                                                       ccgcgcccgcTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCgg               >  2:76431/1‑149 (MQ=255)
                                                                                                                                        cgcgcccgcTTGTGCAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGt              >  5:90807/1‑149 (MQ=255)
                                                                                                                                        cgcgcccgcTTGTGCAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGt              >  2:259999/1‑149 (MQ=255)
                                                                                                                                         gcgcccgcTTGTGCAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTg             >  4:117391/1‑149 (MQ=255)
                                                                                                                                         gcgcccgcTTGTGCAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCAGAGGCGGTg             >  7:263324/1‑149 (MQ=255)
                                                                                                                                            cccgcTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTg          <  7:120261/149‑1 (MQ=255)
                                                                                                                                              cgcTTGTGCAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGc        <  6:90807/149‑1 (MQ=255)
                                                                                                                                              cgcTTGCACAAGCGGGCGCGGGGTTTTTTTCTTTATCAAGGCACCATTCCTTTTCATGAGTTATCCATGAAAATCGCTATCGCCCTGTGTTCTCCCCGGCCCATGTGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTg          <  1:121891/149‑1 (MQ=255)
                                                                                                                                                    cacaAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGCcggcgg  <  2:303408/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
GCGCACATGCCGGCGGCGATTCGCAATGTGCTGGAGTCGGGTGTGGCTACCGGGGGGATTTGTGCGCTGGTGCTGAACTGGTTCTTGCCGGAGGGCAAGGAGCAGGCTTGAACCTTGAGACTGAATGAGAGTAGCCCGCGCCCGCTTGCACAAGCGGGCGCGGGCTTTTTTGCTTTATCATGGCACCATTCCTTTGCATGAGTTAGCCATGAAATTCGCTATCG‑‑CGGTGTTCTCCCCGGCCCATGCGCCCTCTTCGCGACGCGCCCTGCGCTTCGCCGAGGCGGTGCTGGCCGGCGG  >  NC_002947/4503641‑4503937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑