Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,530,026 T→A 10.1% intergenic (+32/‑136) PP_4018 → / → topB acetyltransferase/DNA topoisomerase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,530,0260TA10.1% 111.2 / 6.2 50intergenic (+32/‑136)PP_4018/topBacetyltransferase/DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base T (23/22);  new base A (2/3);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.69e-01

GGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATGCGCCTGTTTC  >  NC_002947/4529886‑4530174
                                                                                                                                            |                                                                                                                                                    
ggTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGa                                                                                                                                              <  6:398860/149‑1 (MQ=255)
 gTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCGCCTGAGCCGGCCTCTTCGCGGGTAAACCCGCGaa                                                                                                                                             <  5:248216/149‑1 (MQ=39)
  tGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGTATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAag                                                                                                                                            <  2:152362/149‑1 (MQ=255)
    cacgccacgcCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAagag                                                                                                                                          >  7:2926/1‑149 (MQ=255)
    cacgccacgcCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAaga                                                                                                                                           <  6:192607/148‑1 (MQ=255)
    cacgccacgcCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCAGGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCGCCTGAGCCGGCCTCTTCGCGGGTAAACCCGCGAagag                                                                                                                                          <  7:300793/149‑1 (MQ=38)
         cacgccGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGCTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCCAAGAGGCCgc                                                                                                                                     >  1:226336/1‑148 (MQ=255)
                  cTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGa                                                                                                                            >  5:161268/1‑149 (MQ=255)
                     tGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTGCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGat                                                                                                                           <  5:319592/147‑1 (MQ=255)
                           ggtGGGGTTTCGCTTAGTCAGCGAGTAATTCCTCGAAGCTGGCAGCCCGCATGTCGACATGGTGCGCGACGCGCGCGCCTTATCCATCACCTCCACTGGCCTCTTCGCGGTTTTCCCCGCTAAGAGGCCGGCTCAGGCGATATACATcc                                                                                                                   <  8:118375/147‑1 (MQ=255)
                             tGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTAc                                                                                                                                                    <  2:185877/114‑1 (MQ=255)
                             tGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTAc                                                                                                                                                    >  1:185877/1‑114 (MQ=255)
                                           gTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTAAACCCGCGaa                                                                                                                                             >  1:61646/1‑107 (MQ=255)
                                           gTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTAAACCCGCGaa                                                                                                                                             <  2:61646/107‑1 (MQ=255)
                                                 gAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTAca                                                                                             >  6:142755/1‑149 (MQ=255)
                                                           tCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTAcatccatc                                                                                       >  6:294867/1‑145 (MQ=255)
                                                                  tGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTAGCGGGGTTACCCGCGAAGAGGCCGGCTAAGGCGATATAAATACCCGCAGACAAAAAAACTGTACATGCCTCCAGATAAAAca                                                                            >  1:390852/1‑148 (MQ=255)
                                                                     cATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCTAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGc                                                                         <  5:189162/149‑1 (MQ=255)
                                                                      aTCCGGCTTGTCTACTTGGTGCGCTACTCGCGCGCCTTATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGcc                                                                        <  6:161268/149‑1 (MQ=255)
                                                                      aTCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACa                                                                                                                      >  7:391576/1‑103 (MQ=255)
                                                                      aTCCCGATTGTCGACTTGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACa                                                                                                                      <  8:391576/103‑1 (MQ=255)
                                                                         ccGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTAGCGGGTTTAACCGCGAAGAGGCCGGCTCAGGCGATATACATCACCGCTGACAAAAAAACTGTACCTCCATCCCGATAAAACTTGCctct                                                                     >  8:282209/1‑149 (MQ=255)
                                                                          cGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACATGCCTCTg                                                                    >  1:157672/1‑149 (MQ=255)
                                                                                   aCATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGaa                                                                                                                                             >  3:52433/1‑67 (MQ=255)
                                                                                   aCATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGaa                                                                                                                                             <  4:52433/67‑1 (MQ=255)
                                                                                     aTGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAAACTGTACATCCATCCAGATAAAACATGCCTCTGAGCCCCCCCtt                                                         >  1:5909/1‑149 (MQ=255)
                                                                                        tggcgcGACTCGCGCGCCTGTTCCAGCACCTTCCGTGGCCTCTGCGCGGGTTTACCCGCTATGCGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTgc                                                       <  2:5909/146‑1 (MQ=255)
                                                                                        gTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTgcg                                                      >  1:359375/1‑149 (MQ=255)
                                                                                           cgcgACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCAt                                                   <  2:359375/149‑1 (MQ=255)
                                                                                            gcgACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGc                                                                         <  1:234892/126‑1 (MQ=255)
                                                                                            gcgACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGc                                                                         >  2:234892/1‑126 (MQ=255)
                                                                                            gcgACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACATGCCTCTGCGCCCCCGCTTGCGCATc                                                  >  7:275807/1‑149 (MQ=255)
                                                                                               acgcccgcgcCTGATCCAAGACCTGCAGTGCCCTCTTCGGGGGTTTACCCGCGAATTGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTa                                               <  8:275807/144‑1 (MQ=255)
                                                                                                     gcgcCTGATCCATCACCTGCACTGGCCTCTTCGCCGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGccc                                         <  7:192402/149‑1 (MQ=255)
                                                                                                                aTCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGAt                              >  5:336918/1‑149 (MQ=255)
                                                                                                                 tCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGAt                              >  8:130750/1‑148 (MQ=255)
                                                                                                                    ccTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTTCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAg                          <  5:294867/149‑1 (MQ=255)
                                                                                                                    ccTGCACTGGCCTATTAGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATAAATCCCCCCTGACAAAAAACCAGTACATCCAACCAGATAAAACTTGCCTCTGCGCCCCCCCTTGCGCATCCTATTGCCCCCCACCCCgaagaac                          >  4:393724/1‑148 (MQ=255)
                                                                                                                     cTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGc                         >  1:108083/1‑149 (MQ=255)
                                                                                                                        cACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTc                      <  7:130750/149‑1 (MQ=255)
                                                                                                                           tGGCCTCTTCGCGGGTTTACCCGCGAAGAGCCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCAATGGCCCACCACCCCGATGAAGCGTcccc                   >  1:32954/1‑149 (MQ=255)
                                                                                                                             gCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCgg                 <  7:292021/149‑1 (MQ=255)
                                                                                                                               ctctTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATc                                                  <  3:13598/114‑1 (MQ=255)
                                                                                                                               ctctTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATc                                                  >  4:13598/1‑114 (MQ=255)
                                                                                                                                tctTCGCGGGTAAACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCa              >  6:103765/1‑149 (MQ=255)
                                                                                                                                 ctTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACatccatcc                                                                                      >  6:337701/1‑76 (MQ=255)
                                                                                                                                 ctTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACatccatcc                                                                                      <  5:337701/76‑1 (MQ=255)
                                                                                                                                    ggcgGGTTTACCCGCTATGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATgc           <  6:336918/147‑1 (MQ=255)
                                                                                                                                        ggTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCAccc                                  >  2:194501/1‑121 (MQ=255)
                                                                                                                                        ggTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCAccc                                  <  1:194501/121‑1 (MQ=255)
                                                                                                                                            tACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTccc                    <  1:74732/131‑1 (MQ=255)
                                                                                                                                            tACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTccc                    >  2:74732/1‑131 (MQ=255)
                                                                                                                                            tACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATGCGCCTGTTtc  >  5:152464/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
GGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCATCACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATGCGCCTGTTTC  >  NC_002947/4529886‑4530174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑