Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,576,129 C→T 7.2% P176S (CCG→TCG)  ybhP‑II → phosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,576,1290CT 7.2% 162.7 / 2.3 55P176S (CCG→TCG) ybhP‑IIphosphohydrolase
Reads supporting (aligned to +/- strand):  ref base C (23/28);  new base T (2/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

ACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGCACGAAGCCTTCGAGCGCCACCACGGCCACCTCGCGCGCACCTACCCGGCCCGCCTGCCG  >  NC_002947/4575982‑4576275
                                                                                                                                                   |                                                                                                                                                  
aCCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGcc                                                                                                                                                   >  2:123850/1‑149 (MQ=255)
aCCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGc                                                                                                                                                    <  3:208460/148‑1 (MQ=255)
aCCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGc                                                                                                                                                    <  2:125362/148‑1 (MQ=255)
           aCGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGccc                                                                                                                                        <  3:13014/149‑1 (MQ=255)
               cgcgGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGt                                                                                                                                    <  6:273155/149‑1 (MQ=255)
               cgcgGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGt                                                                                                                                    <  3:406675/149‑1 (MQ=255)
                 cgGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGa                                                                                                                                  <  6:121650/149‑1 (MQ=255)
                   tccTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAACGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACTCCCTGCCGGCCAACGCCCCGGTGatc                                                                                                                                <  3:353001/148‑1 (MQ=255)
                   gCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGatc                                                                                                                                <  3:124/149‑1 (MQ=255)
                   gCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGatc                                                                                                                                <  4:42191/149‑1 (MQ=255)
                    ccTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGTCGGCCAACGCCCCGGTGat                                                                                                                                 >  7:225087/1‑147 (MQ=255)
                    ccTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGTCGGCCAACGCCCCGGTGat                                                                                                                                 <  8:218990/147‑1 (MQ=255)
                    ccTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGTCGGCCAACGCCCCGGTGat                                                                                                                                 <  8:225087/147‑1 (MQ=255)
                    ccTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGTCGGCCAACGCCCCGGTGat                                                                                                                                 >  7:218990/1‑147 (MQ=255)
                        ttGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGatcatc                                                                                                                             <  5:142212/147‑1 (MQ=255)
                          gCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCg                                                                                                                         >  1:92688/1‑149 (MQ=255)
                                            gCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCCCCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTgg                                                                                                        >  7:368529/1‑148 (MQ=255)
                                             ccGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGa                                                                                                       >  2:276075/1‑148 (MQ=255)
                                              cGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGatc                                                                                                                                <  1:148231/122‑1 (MQ=255)
                                              cGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGatc                                                                                                                                >  2:148231/1‑122 (MQ=255)
                                               ggggCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGaa                                                                                                      >  1:152646/1‑147 (MQ=255)
                                                  gCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCgg                                                                                                                                     >  6:244815/1‑113 (MQ=255)
                                                  gCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCgg                                                                                                                                     <  5:244815/113‑1 (MQ=255)
                                                   cAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCa                                                                                                >  1:273572/1‑149 (MQ=255)
                                                     aGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTTatc                                                                                                                                >  6:288469/1‑115 (MQ=255)
                                                     aGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTTatc                                                                                                                                <  5:288469/115‑1 (MQ=255)
                                                      ggCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATgg                                                                                             <  5:382973/149‑1 (MQ=255)
                                                         cAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCaa                                                                                          <  8:81928/149‑1 (MQ=255)
                                                         cAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCaa                                                                                          >  5:99188/1‑149 (MQ=255)
                                                          aGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAAc                                                                                         <  4:17045/149‑1 (MQ=255)
                                                          aGGTGCACGCAGACCGCATGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGatca                                                                                                                               <  5:229327/111‑1 (MQ=255)
                                                          aGGTGCACGCAGACCGCATGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGatca                                                                                                                               >  6:229327/1‑111 (MQ=255)
                                                                 tGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCAccc                                                                                  >  3:337905/1‑149 (MQ=255)
                                                                          cGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACt                                                                                                                   <  5:260509/107‑1 (MQ=255)
                                                                          cGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACt                                                                                                                   >  6:260509/1‑107 (MQ=255)
                                                                          cGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCt                                                                           <  2:152646/147‑1 (MQ=255)
                                                                          cGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTg                                                                          <  1:276075/148‑1 (MQ=255)
                                                                                      gTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGc                                                             <  4:337905/149‑1 (MQ=255)
                                                                                       ttGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCAGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGCa                                                            >  1:305361/1‑149 (MQ=255)
                                                                                           tGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATg                                                                                              >  7:10329/1‑111 (MQ=255)
                                                                                           tGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATg                                                                                              <  8:10329/111‑1 (MQ=255)
                                                                                                  ccACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGCACGAAGCCTTc                                                  >  8:272148/1‑148 (MQ=255)
                                                                                                   cACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGCACGAAGCCTTc                                                  >  7:210494/1‑147 (MQ=255)
                                                                                                     ccGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGCACGAAGCCTTCGAgc                                              >  2:204735/1‑149 (MQ=255)
                                                                                                                agcTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGCACGAAGCCTTCGAGCGCCACCACGGc                                   >  8:40081/1‑149 (MQ=255)
                                                                                                                        ttGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGCACGAAGCCTTCGAGCGCCACCACGGCCACCTcgc                           <  3:331497/149‑1 (MQ=255)
                                                                                                                           ctgcGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGCACGAAGCCTTCGAGCGCCACCACGGCCACCTcgcgc                         <  1:123850/148‑1 (MQ=255)
                                                                                                                              cGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGCACGAAGCCTTCGAGCGCCACCACGGCCACCTCgcgcgc                       <  6:99188/147‑1 (MQ=255)
                                                                                                                                tAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGCACGAAGCCTTCGAGCGCCACCACGGCCACCTCGCGCGCAcc                    >  1:298325/1‑148 (MQ=255)
                                                                                                                                 aaaCTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCg                                                                                                                         >  7:242169/1‑46 (MQ=255)
                                                                                                                                 aaaCTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCg                                                                                                                         <  8:242169/46‑1 (MQ=255)
                                                                                                                                           aCGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGCACGAAGCCTTCGAGCGCCACCACGGCCACCTCGCGCGCACCTACCCGGCCCGc        <  7:272148/149‑1 (MQ=255)
                                                                                                                                               ccTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGCACGAAGCCTTCGAGCGcca                                          <  8:220062/111‑1 (MQ=255)
                                                                                                                                               ccTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGCACGAAGCCTTCGAGCGcca                                          >  7:220062/1‑111 (MQ=255)
                                                                                                                                               ccTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGCACGAAGCCTTCGAGCGCCACCACGGCCACCTCGCGCGCACCTACCCGGCCCGCCTGc    <  1:204735/149‑1 (MQ=255)
                                                                                                                                                 tGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCtt                                                                                >  5:29250/1‑71 (MQ=255)
                                                                                                                                                 tGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCtt                                                                                <  6:29250/71‑1 (MQ=255)
                                                                                                                                                 tGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGCACGAAGCCTTCGAGCGCCACCACGGCCACCTCGCGCGCACCTACCCGGCCCGCCTGCCg  >  7:246286/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCTTGGCCTGCAACGCGACTTGCACGAAGCCTTCGAGCGCCACCACGGCCACCTCGCGCGCACCTACCCGGCCCGCCTGCCG  >  NC_002947/4575982‑4576275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑