Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,605,508 G→C 5.8% E308D (GAG→GAC) ‡ PP_4077 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,605,5080GC 5.8% 219.1 / 4.3 69E308D (GAG→GAC) ‡PP_4077hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (31/33);  new base C (2/2);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

CCACAGGTGGGCATCGAGTTCCATTTCACGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGACTTTTCGCCCTTTCGAATCGTTC  >  NC_002947/4605368‑4605652
                                                                                                                                            |                                                                                                                                                
ccACAGGTGGGCATCGAGTTCCATTTCACGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGcc                                                                                                                                            >  7:73969/1‑147 (MQ=255)
    aGGTGGGCATCGAGTTCCATTTCACGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTc                                                                                                                                      <  5:121169/149‑1 (MQ=255)
      gTGGGCATCGAGTTCCATTTCACGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGa                                                                                                                                    <  8:357462/149‑1 (MQ=255)
      gTGGGCATCGAGTTCCATTTCACGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGa                                                                                                                                    <  8:351833/149‑1 (MQ=255)
       tGGGCATCGAGTTCCATTTCACGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGGCAGCCTCGCTGGCCAGGTCGat                                                                                                                                   <  3:392944/149‑2 (MQ=37)
         ggCATCGAGTTCCATTTCACGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCt                                                                                                                                 <  7:162571/149‑1 (MQ=255)
            aTCGAGTTCCATTTCACGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGaa                                                                                                                              <  2:197238/149‑1 (MQ=255)
            aTCGAGTTCCATTTCACGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTACACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCCGCCCAGGTCGAGCTg                                                                                                                                >  2:200062/1‑147 (MQ=255)
                    ccATTTCACGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAg                                                                                                                        >  1:329017/1‑147 (MQ=255)
                      aTTTCACGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGc                                                                                                                    >  2:212263/1‑149 (MQ=255)
                       tttCACGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGc                                                                                                                    >  5:45885/1‑148 (MQ=255)
                       tttCACGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGc                                                                                                                    >  5:411064/1‑148 (MQ=255)
                           aCGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGTCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAgg                                                                                                                <  8:24931/148‑1 (MQ=255)
                           aCGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGgc                                                                                                               <  6:45885/149‑1 (MQ=255)
                              cgTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCa                                                                                                            <  1:325764/149‑1 (MQ=255)
                                tCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGt                                                                                                          >  8:227316/1‑149 (MQ=255)
                                   aCTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCg                                                                                                       <  7:227316/149‑1 (MQ=255)
                                       ccAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCtt                                                                                                    <  3:181357/148‑1 (MQ=255)
                                        cAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTAc                                                                                                  <  1:359833/149‑1 (MQ=255)
                                             acCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGgcg                                                                                                              >  4:200308/1‑132 (MQ=255)
                                             acCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGgcg                                                                                                              <  3:200308/132‑1 (MQ=255)
                                               ccTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCa                                                                                                                                          >  6:306486/1‑102 (MQ=255)
                                               ccTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCa                                                                                                                                          <  5:306486/102‑1 (MQ=255)
                                                cTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCTAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCtt                                                                                          >  3:33015/1‑149 (MQ=255)
                                                 ttCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCtt                                                                                          >  8:7935/1‑148 (MQ=255)
                                                   cgcgTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCa                                                                                        <  2:329017/148‑1 (MQ=255)
                                                      gTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCCAGCTGAAGCCCAGAAGCGAGACGCAGTCCGCTTACGAAATCTTCAgtg                                                                                     >  4:207729/1‑148 (MQ=255)
                                                                aagaTTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGt                                                                           >  6:279765/1‑148 (MQ=255)
                                                                    tttCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGtcatc                                                                       <  5:279765/148‑1 (MQ=255)
                                                                             gTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGa                                                              >  2:298859/1‑148 (MQ=255)
                                                                                 tGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGgcgc                                                                                                             <  5:356373/97‑1 (MQ=255)
                                                                                 tGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGgcgc                                                                                                             >  6:356373/1‑97 (MQ=255)
                                                                                  gCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGaacaac                                                         >  5:189002/1‑148 (MQ=255)
                                                                                   cACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGAt                                                                                <  1:369876/124‑1 (MQ=255)
                                                                                   cACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGAt                                                                                >  2:369876/1‑124 (MQ=255)
                                                                                   cACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGaacaac                                                         <  4:353360/147‑1 (MQ=255)
                                                                                   cACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGaacaac                                                         >  3:353360/1‑147 (MQ=255)
                                                                                   cACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGaacaa                                                          <  6:125302/146‑1 (MQ=255)
                                                                                   cACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCa                                                       <  6:411064/149‑1 (MQ=255)
                                                                                    aCCCCGTCTGTCATCTTGTTGAATCACAGTGCCGATCCCATCGACCTGGCCATCGTTGCTGACCATGTCGAGCTGAAGCCCAGAAGCGATGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAAccc                                                       <  3:207729/148‑2 (MQ=255)
                                                                                      cccGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGaa                                                                             >  6:300861/1‑124 (MQ=255)
                                                                                      cccGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACt                                                     >  6:185505/1‑148 (MQ=255)
                                                                                      cccGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACt                                                     >  3:403644/1‑148 (MQ=255)
                                                                                              tCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAg                                             <  4:403644/148‑1 (MQ=255)
                                                                                              tCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCGGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGt                                            <  1:200062/149‑1 (MQ=255)
                                                                                                aaCCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCtt                                                                                          >  2:197129/1‑101 (MQ=255)
                                                                                                aaCCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCtt                                                                                          <  1:197129/101‑1 (MQ=255)
                                                                                                aaCCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTAc                                          >  8:265601/1‑149 (MQ=255)
                                                                                                       tCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCTAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGa                                    <  4:33015/148‑1 (MQ=255)
                                                                                                          aGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTATCGGTGAGCAc                                >  2:186455/1‑149 (MQ=255)
                                                                                                              caGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGc                            <  7:7935/149‑1 (MQ=255)
                                                                                                                   ccGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGa                         <  2:249525/147‑1 (MQ=255)
                                                                                                                   ccGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGa                         >  1:249525/1‑147 (MQ=255)
                                                                                                                   ccGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGAc                        <  1:212263/148‑1 (MQ=255)
                                                                                                                   ccGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGACt                       >  6:331592/1‑149 (MQ=255)
                                                                                                                          cATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGACTTTTCGcc                <  1:298859/149‑1 (MQ=255)
                                                                                                                           aTCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGACTTTTCGccc               >  4:35358/1‑149 (MQ=255)
                                                                                                                            tCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACATGCGGACTTTTCGCCCt              >  5:344991/1‑149 (MQ=255)
                                                                                                                              gACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGACTTTTCGCCCttt            <  8:73969/149‑1 (MQ=255)
                                                                                                                               aCCTGGGCAGCCTCGCTGGCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGtcatc                                                                       <  6:24922/89‑1 (MQ=37)
                                                                                                                               aCCTGGGCAGCCTCGCTGGCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGtcatc                                                                       >  5:24922/1‑89 (MQ=37)
                                                                                                                               aCCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGACTTTTCGCCCTTTc           >  2:323977/1‑149 (MQ=255)
                                                                                                                               aCCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGACTTTTCGCCCTTTc           <  6:189002/149‑1 (MQ=255)
                                                                                                                                ccTGGGCAGCCTCGCTGGCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGACTTTTCGCCCTTTc           >  4:28314/1‑148 (MQ=38)
                                                                                                                                    gCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGACTTTTCGCCCTTTCGAAt       <  5:185505/148‑1 (MQ=255)
                                                                                                                                      cAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGACtt                      <  8:227750/131‑1 (MQ=255)
                                                                                                                                      cAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGACTTTTCGCCCTTTCGAATCGt    >  6:394271/1‑149 (MQ=255)
                                                                                                                                       aGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGACTTTTCGCCCTTTCGAATCGtt   >  2:7158/1‑149 (MQ=255)
                                                                                                                                        gCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGACTTTTCGCCCTTTCGAATCGTTc  <  7:265601/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                
CCACAGGTGGGCATCGAGTTCCATTTCACGCGTCAACTGCCAGACACCCTTCGCGTTGGCACAGAAGATTTCAGCCTGTTCTGCACCCCGGCTGTCAACCTGTTCAAGCACAGTGCCGAGCCCATCGACCTGGCCAGCGAGGCTGCCCAGGTCGAGCTGAAGCCCAGAAGCGAGGCGCAGTCCGCTTACGAAATCTTCAGTGTCGATGAAGTCATCAGCACCCGAACAACCACTGACGGAAGTACCGGTGAGCACCTGCGGACTTTTCGCCCTTTCGAATCGTTC  >  NC_002947/4605368‑4605652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑