| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,705,425 | G→A | 16.9% | intergenic (‑163/+14) | kdpF ← / ← PP_4162 | potassium‑transporting ATPase subunit F/membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,705,425 | 0 | G | A | 16.9% | 114.0 / 15.0 | 53 | intergenic (‑163/+14) | kdpF/PP_4162 | potassium‑transporting ATPase subunit F/membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (26/18); new base A (6/3); total (32/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01 | |||||||||||
ACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCCAGCA > NC_002947/4705282‑4705572 | aCGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCg > 7:386282/1‑149 (MQ=255) ggCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGc > 3:404782/1‑149 (MQ=255) ccGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCt > 7:74091/1‑148 (MQ=255) cGCTTTGGGGAAATTGTCCGA‑GGAGAGGGGTAAAGGGGCGGGCTCGGGGGGTGGGGGTGGGTATAAAGAATGCGTTAAAATCAGCGTTGTGGTCTGCCTGTAAAGGCCTCTTCGCGGGCTTGGCCGCGAAAGGGCCGGTTCAGGGttta > 4:235217/1‑147 (MQ=255) aGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTAttgttg > 1:16176/1‑135 (MQ=255) ccctaggggggCGCGGTGGGGGGGGGGGGGGGTCAATAAAGAATGCGGAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑Gtt < 8:336699/141‑1 (MQ=37) gggCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGgctg > 7:79257/1‑128 (MQ=255) gggCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTATTAGCGGGCTTTCCCGCCAAAAGGCCGGTAAAGGCGTATTGTTTTGGCGGCTTCTGCGCCGCGGTTACgctccc > 6:72168/1‑144 (MQ=255) gagaTCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTATGCATTTAACGGCCTATTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTTTTGTGGCGCCTGATGCGCCCC‑GTTACgg > 6:386824/1‑140 (MQ=255) gagaTCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGAGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTAAAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCtccagtc > 6:146666/1‑148 (MQ=255) gggtgggtATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTTTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTg > 7:377916/1‑146 (MQ=255) ggtgggtATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTa < 2:276731/147‑1 (MQ=255) ggtgggtATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTa > 1:276731/1‑147 (MQ=255) ggcgcgaataAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGc < 3:235217/142‑1 (MQ=255) tataAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTAttgtt > 6:146916/1‑92 (MQ=255) tataAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTAttgtt > 6:153210/1‑92 (MQ=255) aaGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAg > 3:365510/1‑149 (MQ=255) aaGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAg > 6:231839/1‑149 (MQ=255) gTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTAttg < 1:115855/78‑1 (MQ=255) gTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCAGCGAAAGGGCCGTTACAGGCTTAttg > 2:115855/1‑78 (MQ=255) gTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCGGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGaa > 3:234755/1‑147 (MQ=255) ttGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAgg < 8:98739/111‑1 (MQ=255) ttGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAgg > 7:98739/1‑111 (MQ=255) tGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAAt < 6:387009/147‑1 (MQ=255) tGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAAt > 5:387009/1‑147 (MQ=255) ccTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAg < 5:146666/149‑1 (MQ=255) tGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGc > 6:272379/1‑148 (MQ=255) gTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGcc > 5:46499/1‑148 (MQ=255) gTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGcc > 2:125186/1‑148 (MQ=255) cGGCCCTTTCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGt < 5:389127/117‑1 (MQ=37) cGGCCCTTTCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGt > 6:389127/1‑117 (MQ=37) ggccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAgg > 3:391400/7‑148 (MQ=38) ggCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGcc < 6:128596/134‑1 (MQ=255) ggCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGcc > 5:128596/1‑134 (MQ=255) gccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAg > 3:362003/6‑146 (MQ=39) gccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAg < 4:362003/141‑1 (MQ=39) ccctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgc > 8:86916/5‑147 (MQ=39) ccTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaa < 2:267207/149‑1 (MQ=255) ccTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaa < 4:234755/149‑1 (MQ=255) ctttCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaagc < 4:391400/147‑1 (MQ=255) tttCGCGGGCAAGCCCGCGAAGGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGaa > 2:34426/2‑135 (MQ=255) tttCGCGGGCAAGCCCGCGAAGGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGaa < 1:34426/134‑1 (MQ=255) cgcgGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGgcaagcaa < 8:74091/147‑1 (MQ=255) gcgGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAgcc > 6:371416/1‑149 (MQ=255) ggCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAgccgcc < 1:125186/149‑1 (MQ=255) ggCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAgccgcc < 6:46499/149‑1 (MQ=255) aagCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGaa > 5:263118/3‑149 (MQ=255) ccGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGaa < 6:241651/145‑1 (MQ=255) ccGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGaa > 5:241651/1‑145 (MQ=255) ccGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACg > 1:259125/1‑149 (MQ=255) cgAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGccc < 5:371416/149‑1 (MQ=255) aaGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCcagc < 5:72168/149‑1 (MQ=255) aGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCcagca < 5:231839/149‑1 (MQ=255) | ACGGCTTGGGCCGCTTTGGGGAAATTGTCCGAGGGAGAGGCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGC‑GTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCCAGCA > NC_002947/4705282‑4705572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |