Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,730,874 G→A 5.4% G190G (GGC→GGT)  lpdG ← dihydrolipoyl dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,730,8740GA 5.4% 229.6 / 2.1 74G190G (GGC→GGT) lpdGdihydrolipoyl dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (32/38);  new base A (2/2);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TCCAGGCCTTGCTTGGTCAGGGTCTTCTGGGCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGAT  >  NC_002947/4730731‑4731020
                                                                                                                                                |                                                                                                                                                   
tCCAGGCCTTGCTTGGTCAGGGTCTTCTGGGCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTg                                                                                                                                                 <  4:214515/148‑1 (MQ=255)
  cAGGCCTTGCTTGGTCAGGGTCTTCTGGGCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCAc                                                                                                                                              <  3:150778/149‑1 (MQ=255)
   aGGCCTTGCTTGGTCAGGGTCTTCTTGGCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCAcc                                                                                                                                             <  4:168450/149‑1 (MQ=255)
     gCCTTGCTTGGTCAGGGTCTTCTGGGCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCt                                                                                                                                                  >  8:185503/1‑142 (MQ=255)
     gCCTTGCTTGGTCAGGGTCTTCTGGGCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCt                                                                                                                                                  <  7:185503/142‑1 (MQ=255)
     gCCTTGCTTGGTCAGGGTCTTCTGGGCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGa                                                                                                                                           <  8:33189/149‑1 (MQ=255)
     gCCTTGCTTGGTCAGGGTCTTCTGGGCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGa                                                                                                                                           >  6:93181/1‑149 (MQ=255)
          gcttgGTCAGGGTCTTCTGGGCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACg                                                                                                                                      >  1:364425/1‑149 (MQ=255)
              ggTCAGGGTCTTCTGGGCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCCGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGcc                                                                                                                                    <  2:364425/147‑1 (MQ=255)
                tCAGGGTCTTCTGGGCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAgg                                                                                                                                <  3:137834/149‑1 (MQ=255)
                 cAGGGTCTTCTGGGCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGgc                                                                                                                               >  5:129501/1‑149 (MQ=255)
                 cAGGGTCTTCTGGGCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGgc                                                                                                                               >  7:103138/1‑149 (MQ=255)
                     gTCTTCTGGGCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCtt                                                                                                                           <  6:129501/149‑1 (MQ=255)
                          ctGGGCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCg                                                                                                                         <  3:392824/146‑1 (MQ=255)
                            gggCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGa                                                                                                                                           <  3:92983/126‑1 (MQ=255)
                            gggCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGa                                                                                                                                           >  4:92983/1‑126 (MQ=255)
                             ggCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTa                                                                                                                      <  3:52278/146‑1 (MQ=255)
                              gCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGc                                                                                                                  >  7:320345/1‑149 (MQ=255)
                              gCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGc                                                                                                                  <  8:320345/149‑1 (MQ=255)
                               cttcttTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGcc                                                                                                                 >  5:18328/1‑149 (MQ=255)
                                ttcttTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCt                                                                                                                >  3:275903/1‑149 (MQ=255)
                                   tttCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTACAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGc                                                                                                                  <  5:297534/144‑1 (MQ=255)
                                   tttCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTACAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGc                                                                                                                  >  6:297534/1‑144 (MQ=255)
                                      cGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAAtt                                                                                                          >  1:110072/1‑149 (MQ=255)
                                       gACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCACATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑c                                                                                                        >  2:54522/1‑149 (MQ=255)
                                           cggcggTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAgg                                                                                                    >  5:300772/1‑149 (MQ=255)
                                           cggcggTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAgg                                                                                                    <  8:88442/149‑1 (MQ=255)
                                                           cATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAg                                                                                        >  6:216379/1‑145 (MQ=255)
                                                           cATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAg                                                                                        <  5:216379/145‑1 (MQ=255)
                                                           cATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTcga                                                                                    >  4:265816/1‑149 (MQ=255)
                                                             tCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATGACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑cc                                                                                                       >  2:346957/1‑128 (MQ=255)
                                                              cAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTcgacga                                                                                 >  8:58286/1‑149 (MQ=255)
                                                               aGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGAt                                                                                >  3:119143/1‑149 (MQ=255)
                                                                 gAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTgg                                                                                                              <  6:295742/118‑1 (MQ=255)
                                                                 gAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTgg                                                                                                              >  5:295742/1‑118 (MQ=255)
                                                                       gtCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCt                                                                        >  1:334516/1‑149 (MQ=255)
                                                                         ccAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCACATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATTCCCAGGGCGCCGGTGGAGTCGACGATGACGTTCTg                                                                       <  1:54522/149‑1 (MQ=255)
                                                                             ggCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGa                                                                  >  6:95361/1‑149 (MQ=255)
                                                                                ttCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCAGGCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGAcc                                                                >  1:297722/1‑149 (MQ=255)
                                                                                  ccAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCggg                                                             >  3:167383/1‑149 (MQ=255)
                                                                                       aCGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCcgg                                                        >  8:151793/1‑149 (MQ=255)
                                                                                        cGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGATGTTCTGGTCGACCGGGGCcggc                                                       >  5:44212/1‑149 (MQ=255)
                                                                                        cGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCcggc                                                       <  7:151793/149‑1 (MQ=255)
                                                                                           tCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCATGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCcggcgg                                                     >  5:371448/1‑148 (MQ=255)
                                                                                             aCTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAAt                                                  <  7:58286/149‑1 (MQ=255)
                                                                                             aCTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACACCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTCGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAAt                                                  >  1:401915/1‑149 (MQ=255)
                                                                                              cTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATg                                                 <  4:119143/149‑1 (MQ=255)
                                                                                               ttCAGCACCCAGGCGAGCCCATACCGTACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCg                                                                                              >  1:9806/1‑103 (MQ=255)
                                                                                               ttCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGt                                                <  2:334516/149‑1 (MQ=255)
                                                                                               ttCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTAAGGCCTGGAATT‑CCAGGGCGCCg                                                                                              <  2:9806/103‑1 (MQ=255)
                                                                                               ttCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGAACACACCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGTAGTCGACGATGACGTTCTGGTCGCCCGGGGCCGGCGGAATGt                                                >  5:7132/1‑149 (MQ=255)
                                                                                                tCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTc                                               <  3:265816/149‑1 (MQ=255)
                                                                                                 ctgCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCTCACCTGCACCGATAAGGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTc                                               <  6:7132/146‑1 (MQ=255)
                                                                                                 cAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGt                                                <  8:103138/147‑1 (MQ=255)
                                                                                                 cAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTc                                               <  5:93181/148‑1 (MQ=255)
                                                                                                  aGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCg                                              <  4:167383/148‑1 (MQ=255)
                                                                                                  aGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGa                                             >  5:69234/1‑149 (MQ=255)
                                                                                                    cACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATc                                           <  3:49252/149‑1 (MQ=255)
                                                                                                        cAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGgc                                       <  2:297722/149‑1 (MQ=255)
                                                                                                             gAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATg                                                 <  1:40232/134‑1 (MQ=255)
                                                                                                             gAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATg                                                 >  2:40232/1‑134 (MQ=255)
                                                                                                              aGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGc                                 <  6:18328/149‑1 (MQ=255)
                                                                                                                   aTACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTc                                               <  4:200726/130‑1 (MQ=255)
                                                                                                                   aTACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTc                                               >  3:200726/1‑130 (MQ=255)
                                                                                                                      ccGAACCCA‑GCTCCAGGCCGATCACACCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGcc                         <  2:401915/149‑1 (MQ=255)
                                                                                                                       cGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGa                                                                                         >  3:92793/1‑84 (MQ=255)
                                                                                                                       cGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGa                                                                                         <  4:92793/84‑1 (MQ=255)
                                                                                                                            ccA‑GATCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATc                   <  6:69234/149‑1 (MQ=255)
                                                                                                                              a‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCAc                 <  8:349897/149‑1 (MQ=255)
                                                                                                                                    cAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACg                <  8:243742/145‑1 (MQ=255)
                                                                                                                                    cAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACg                >  7:243742/1‑145 (MQ=255)
                                                                                                                                       gCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCgg         <  5:95361/149‑1 (MQ=255)
                                                                                                                                              aCGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGAt  <  1:169280/149‑1 (MQ=255)
                                                                                                                                              aCGCATGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGAt  <  6:371448/149‑1 (MQ=255)
                                                                                                                                                gCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTc     <  8:284584/144‑1 (MQ=255)
                                                                                                                                                gCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTc     >  7:284584/1‑144 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TCCAGGCCTTGCTTGGTCAGGGTCTTCTGGGCTTCTTTCGACACGGCGGTGTCGGCAGCCATCAGGAAGGTGTCCAGGGCTTCCAGGACGGTCACTTCAGCACCCAGGCGAGCCCATACCGAACCCA‑GCTCCAGGCCGATCACGCCTGCACCGATAACGCCCAGGCGCTTCGGTACGGCCTGGAATT‑CCAGGGCGCCGGTGGAGTCGACGATGACGTTCTGGTCGACCGGGGCCGGCGGAATGTCGATCGGGCGCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGAT  >  NC_002947/4730731‑4731020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑