Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,740,816	T→G	100%	intergenic (+53/+700)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,740,816	0	T	G	100.0%	181.8 / NA	54	intergenic (+53/+700)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/27);  total (27/27)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATC  >  NC_002947/4740676‑4740956                                                                                                                                              |                                                                                                                                               ctcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGcc                                                                                                                                       >  1:351734/1‑149 (MQ=255)   ccAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGTGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgg                                                                                                                                     >  1:292235/1‑149 (MQ=255)        ccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct                                                                                                                                <  2:375813/149‑1 (MQ=255)            aCAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgc                                                                                                                            >  1:63652/1‑149 (MQ=255)                    ggCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGc                                                                                                                    >  5:344767/1‑149 (MQ=255)                    ggCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGc                                                                                                                    >  5:4907/1‑149 (MQ=255)                     gCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGcc                                                                                                                   >  8:348315/1‑149 (MQ=255)                           cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGC‑‑CGCGaa                                                                                                           <  5:314787/149‑1 (MQ=255)                            gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAACGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGaa                                                                                                           <  1:328402/149‑1 (MQ=255)                            gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAag                                                                                                          <  2:292235/149‑1 (MQ=255)                             cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAaga                                                                                                         <  3:83793/149‑1 (MQ=255)                             cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCACTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAaga                                                                                                         <  5:13022/149‑1 (MQ=255)                              gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGC‑‑CGCGAagag                                                                                                        <  5:58332/149‑1 (MQ=38)                              gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAagag                                                                                                        <  7:108256/149‑1 (MQ=255)                              gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAagag                                                                                                        <  2:69553/149‑1 (MQ=255)                                 aGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGcc                                                                                                     >  4:87251/1‑149 (MQ=255)                                        cacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGcaca                                                                                               <  6:344767/148‑1 (MQ=255)                                         acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGC‑‑CGCGaa                                                                                                           >  7:145575/1‑135 (MQ=38)                                         acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGC‑‑CGCGaa                                                                                                           <  8:145575/135‑1 (MQ=38)                                          ccGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGC‑‑CGCGAagag                                                                                                        >  5:356868/1‑137 (MQ=38)                                          ccGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGC‑‑CGCGAagag                                                                                                        <  6:356868/137‑1 (MQ=38)                                                   aGACGCGCGAAATCGTGGCCGTTAAACCCCCCACTAACGCGGCAGTCCCCCAACCCAAC‑ACGGGTGCCCGCGGGCAG‑CTTTTTTTATTTGGCCTGGGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACaaaa                                                                                   <  2:351734/149‑1 (MQ=255)                                                   aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGcc                                                                                                                        >  2:225692/1‑114 (MQ=255)                                                   aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGC‑‑CGCGAAGAGGc                                                                                                      <  5:374177/130‑1 (MQ=37)                                                   aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGC‑‑CGCGAAGAGGc                                                                                                      >  6:374177/1‑130 (MQ=37)                                                    gAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCCTTTTTTT‑TTTGTCCTGTGCCGGCCTCTTCGCGGCCATGCC‑‑CGCGAAGCGGCCGGCCCCGACACCCCAAAAc                                                                                  >  3:74225/1‑149 (MQ=255)                                                        cgcgACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAACCt                                                                                >  5:419855/1‑147 (MQ=255)                                                           gACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGACAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGtt                                                                           >  1:328995/1‑149 (MQ=255)                                                               tCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGC‑‑CGCGAaga                                                                                                         <  2:386189/115‑1 (MQ=37)                                                               tCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGC‑‑CGCGAaga                                                                                                         >  1:386189/1‑115 (MQ=37)                                                                      ccTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTTCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAACCACAGATTTTATccgccc                                                                >  8:161000/1‑149 (MQ=255)                                                                         gAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcg                                                                                                                             <  4:128222/87‑1 (MQ=255)                                                                         gAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcg                                                                                                                             >  3:128222/1‑87 (MQ=255)                                                                         gAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAAc                                                                                  >  6:195303/1‑128 (MQ=39)                                                                         gAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAAc                                                                                  <  5:195303/128‑1 (MQ=39)                                                                            aGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGGGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCAc                                                          <  7:161000/149‑1 (MQ=255)                                                                                         gCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGCCCCGCACAGACCCCACAAAACCTCac                                                                             >  5:420508/1‑116 (MQ=255)                                                                                           tGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGGCCAGCCACCACAAACCCTCAGTTTTTCTCCGCCCCCTCACGCAGCCCTGTAAggg                                           >  3:300727/1‑149 (MQ=255)                                                                                            gCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCCGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGCCACCCCAAAACCTCAGTTTTTATCCCCCCGCTCACGCAGCCCGTTCAggg                                           >  6:157603/1‑148 (MQ=255)                                                                                               gTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAtt                                       <  6:419855/149‑1 (MQ=255)                                                                                                 aGCCGAACGCAAG‑AAGGCTGCCCTCGGGCATCC‑TTTTTTATTTGTCCTGTGCCGGCATCTTCGCGGCCTTGCCCTCGCTATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAtt                                       <  1:126550/149‑1 (MQ=255)                                                                                                     gAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCGCACGCAGCCCGTTCAGGGAATTGAACgg                                 >  5:374007/1‑149 (MQ=255)                                                                                                     gAACGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACATAGCGGGCATGGC‑‑CGCGAATAGGGCGGGCCCGAAAACCCAAAACCACACTTTTTATACCCCCCCGCACCCCCCCCGTTAAAGGTATTTAAAgg                                 >  2:340994/1‑149 (MQ=255)                                                                                                      aaCGCAAG‑AAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTGGCGGCCTTGCC‑‑CGCGATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgc                                <  7:348315/149‑1 (MQ=255)                                                                                                          taaaaaagGCTGCCCGAGGGCAGCC‑TTCTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCa                             <  2:95878/144‑1 (MQ=255)                                                                                                           aag‑aagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAACCACAGTTTTTATACGCCCGCTCACGCAGCCCTTTCAGGGTATTTAACGGCGCATc                           >  6:363325/1‑149 (MQ=255)                                                                                                                agGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCa                                              <  6:169664/126‑1 (MQ=39)                                                                                                                agGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCa                                              >  5:169664/1‑126 (MQ=39)                                                                                                                  gcggCCCTCGGGAAG‑CTTTTTTTATTTGGCCTGGGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCcaccac                     <  6:374007/146‑1 (MQ=255)                                                                                                                      cccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAATGGCGCATCCACCACGAAc                 >  6:179483/1‑149 (MQ=255)                                                                                                                        cTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAATGGCGCATCCACCACGAACtt               <  5:179483/149‑1 (MQ=255)                                                                                                                          ctatctgCCTTTTTTT‑TTTGTCCTGTGCGGGCCTCTTCGCGGCCTTGCC‑‑CGGGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGTTATTGAACGGCTCATCCACCACGAACttg              <  1:263214/142‑1 (MQ=255)                                                                                                                            ggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg           <  2:63652/149‑1 (MQ=255)                                                                                                                                      ttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATc  >  1:308012/1‑149 (MQ=255)                                                                                                                                              |                                                                                                                                               CTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAG‑AAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCC‑‑CGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATC  >  NC_002947/4740676‑4740956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑