Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,888,343 C→G 5.1% H468D (CAC→GAC)  gcl → glyoxylate carboligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,888,3430CG 5.1% 260.2 / 3.9 79H468D (CAC→GAC) gclglyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base C (27/47);  new base G (2/2);  total (30/49)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01

GGCCCGCTGGGCTGGACCATCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCAACTCGACCGACGCTGCCACCTACGGTGTCGACCACGTCGCTGTGGTCG  >  NC_002947/4888196‑4888487
                                                                                                                                                   |                                                                                                                                                
ggccCGCTGGGCTGGA‑CAT‑CCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACg                                                                                                                                               <  4:282273/149‑1 (MQ=255)
 gccCGCTGGGCTGGACCTTCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCAc                                                                                                                                                <  4:50983/149‑1 (MQ=255)
 gccCGCTGGGCTGGACCACCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCAc                                                                                                                                                <  2:79213/149‑1 (MQ=255)
 gccCGCTGGGCGGGACCATCCCTGCTGCGCTGGGCGTTGGCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCAc                                                                                                                                                <  5:115561/149‑1 (MQ=255)
  cccGCTGGGCTGGACCATCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACg                                                                                                                                               >  6:313460/1‑149 (MQ=255)
    cGCTGGGCTGGACCATCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTa                                                                                                                                             <  3:333756/149‑1 (MQ=255)
    cGCTGGGCTGGACCATCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTa                                                                                                                                             <  6:318141/149‑1 (MQ=255)
        gggctggACCATCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTg                                                                                                                                          <  8:289143/148‑1 (MQ=255)
        gggctggACCATCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTg                                                                                                                                          >  7:289143/1‑148 (MQ=255)
         ggctggACCATCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTgg                                                                                                                                         <  2:336818/148‑1 (MQ=255)
         ggctggACCATCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGt                                                                                                                                        >  1:23765/1‑149 (MQ=255)
          gctggACCATCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTGGACGTAcggcag                                                                                                                                       <  5:305287/149‑6 (MQ=255)
          gctggACCATCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTg                                                                                                                                       <  5:282582/149‑1 (MQ=255)
              gACCATCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGaac                                                                                                                                    >  5:142233/1‑148 (MQ=255)
                ccATCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGaacaac                                                                                                                                 <  5:261334/149‑1 (MQ=255)
                ccATCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGaacaac                                                                                                                                 <  2:48375/149‑1 (MQ=255)
                ccATCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGaacaac                                                                                                                                 <  2:54492/149‑1 (MQ=255)
                  aTCCCTGCTGCGCTGGGCTTGGTCAAAGCCGATCCGAAGCGCAAGGGTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGc                                                                                                                               <  8:115894/149‑1 (MQ=255)
                     ccTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCt                                                                                                                             <  3:378871/148‑1 (MQ=255)
                     ccTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTa                                                                                                                            >  1:290809/1‑149 (MQ=255)
                      ctgctgCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTAc                                                                                                                           >  5:228029/1‑149 (MQ=255)
                      ctgctgCGCTGGCCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGATCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTAc                                                                                                                           >  4:328189/1‑149 (MQ=255)
                             gcTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTAc                                                                                                                           <  2:138949/142‑1 (MQ=255)
                             gcTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTAc                                                                                                                           >  1:138949/1‑142 (MQ=255)
                              cTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCt                                                                                                                   <  3:220112/149‑1 (MQ=255)
                                   cGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATcc                                                                                                              >  1:246129/1‑149 (MQ=255)
                                   cGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATcc                                                                                                              <  2:246129/149‑1 (MQ=255)
                                      ggTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGc                                                                                                                     <  4:384495/139‑1 (MQ=255)
                                      ggTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGc                                                                                                                     >  3:384495/1‑139 (MQ=255)
                                             gCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCa                                                                                                    <  7:242662/149‑1 (MQ=255)
                                              ccGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAg                                                                                                   <  5:313460/149‑1 (MQ=255)
                                               cGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGc                                                                                                  <  2:23765/149‑1 (MQ=255)
                                                gATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTGGACGTAcggcag                                                                                                                                       <  5:302352/111‑6 (MQ=255)
                                                gATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTGGACGTAcggcag                                                                                                                                       >  6:302352/1‑106 (MQ=255)
                                                   ccGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTgg                                                                                              >  2:103072/1‑149 (MQ=255)
                                                     gAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCt                                                                                            >  4:109707/1‑149 (MQ=255)
                                                     gAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCt                                                                                            >  6:44480/1‑149 (MQ=255)
                                                       aGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTc                                                                                          <  6:228029/149‑1 (MQ=255)
                                                         cgcAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGa                                                                                        <  5:155177/149‑1 (MQ=255)
                                                          gcAAGGTTGTGGCGCTGTCGGCTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGa                                                                                        >  2:378444/1‑148 (MQ=255)
                                                           cAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACa                                                                                      <  3:318821/149‑1 (MQ=255)
                                                           cAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACa                                                                                      >  4:318821/1‑149 (MQ=255)
                                                            aaGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACAt                                                                                     <  5:231675/149‑1 (MQ=255)
                                                            aaGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACAt                                                                                     >  3:109046/1‑149 (MQ=255)
                                                             aGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATg                                                                                    <  6:100917/149‑1 (MQ=255)
                                                               gTTGTGGCGCTGTCGGGTTACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGa                                                                                  <  7:379425/149‑1 (MQ=255)
                                                                  gtgGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTg                                                                                                                                          <  8:324526/90‑1 (MQ=255)
                                                                  gtgGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTg                                                                                                                                          >  7:324526/1‑90 (MQ=255)
                                                                   tgGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTa                                                                               <  2:341591/148‑1 (MQ=255)
                                                                     gcgcTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACt                                                                             <  2:290809/148‑1 (MQ=255)
                                                                      cgcTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACtgt                                                                           <  2:359260/149‑1 (MQ=255)
                                                                             gggTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAAc                                                                    >  4:215736/1‑149 (MQ=255)
                                                                               gTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTg                                                                                               >  4:197967/1‑120 (MQ=255)
                                                                               gTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTg                                                                                               <  3:197967/120‑1 (MQ=255)
                                                                               gTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACtgt                                                                           <  5:181778/140‑1 (MQ=255)
                                                                               gTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACtgt                                                                           >  6:181778/1‑140 (MQ=255)
                                                                                tGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTgg                                                                 >  8:16845/1‑149 (MQ=255)
                                                                                  aCTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGc                                                                <  5:47805/148‑1 (MQ=255)
                                                                                   cTACGACTTCCAGTTCATGATCGAAGAACTGGCCGTAGGCGCCCACATCAACCTCCCGTACGTCAACCTACTGTTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCCTGGCTACGACATGGATTACTGAGTACAACAGGCg                                                               >  7:153409/1‑148 (MQ=255)
                                                                                    tACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGtt                                                             >  6:131395/1‑149 (MQ=255)
                                                                                       gACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCga                                                          <  1:103072/149‑1 (MQ=255)
                                                                                       gACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCga                                                          <  1:378444/149‑1 (MQ=255)
                                                                                        aCTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCgag                                                         <  3:109707/149‑1 (MQ=255)
                                                                                         cTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCgaga                                                        <  3:215736/149‑1 (MQ=255)
                                                                                          ttCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCACGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGaa                                                       >  1:135411/1‑149 (MQ=255)
                                                                                                      aTCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCAACTcga                                            <  5:44480/148‑1 (MQ=255)
                                                                                                      aTCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCAACTcga                                            <  7:16845/148‑1 (MQ=255)
                                                                                                      aTCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCAACTcga                                            <  4:109046/148‑1 (MQ=255)
                                                                                                         gaagaaCTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCaa                                                 >  2:218906/1‑140 (MQ=255)
                                                                                                         gaagaaCTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCaa                                                 <  1:218906/140‑1 (MQ=255)
                                                                                                          aagaaCTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCAACTcgaccgac                                       >  3:219420/1‑149 (MQ=255)
                                                                                                                            cccAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCAACTCGACCGACGCTGCCACCTACGGTGTc                     <  3:328189/149‑1 (MQ=255)
                                                                                                                             ccAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCAACTCGACCGACGCTGCCACCTACGGTGTCg                    <  6:142233/149‑1 (MQ=255)
                                                                                                                               aGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCAACTCGACCGACGCTGCCACCTACGGTGTCGAc                  <  4:219420/149‑1 (MQ=255)
                                                                                                                                     aCCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCAACTCGACCGACGCTGCCACCTACGGTGTCGACCACGTc            >  6:320608/1‑149 (MQ=255)
                                                                                                                                      ccTGCCGTACGTCCACGTACTGGTGAACAACGCCTCCCTGGGCCTGCTCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCAACTCGACCGACGCTGCCACCTACGGTGTCGACCACGTCg           <  6:199698/149‑1 (MQ=255)
                                                                                                                                        accagTACGTGGACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCAACTCGACCGACGCTGCCACCTACGGTGTCGACCACGTCGCt         >  5:216498/5‑149 (MQ=255)
                                                                                                                                           cGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCAACTCGACCGACGCTGCCACCTACGGTGTCGACCACGTCGCtgtg      <  5:131395/149‑1 (MQ=255)
                                                                                                                                                 tCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCAACTCGACCGACGCTGCCACCTACGGTGTCGACCACGTCGCTGTGGTCg  >  7:69817/1‑147 (MQ=255)
                                                                                                                                                  ccACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCAACTCGACCGACGCTGCCACCTACGGTGTCGACCACGTCGCTGTGGt    <  1:362024/144‑1 (MQ=255)
                                                                                                                                                  ccACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCAACTCGACCGACGCTGCCACCTACGGTGTCGACCACGTCGCTGTGGt    >  2:362024/1‑144 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GGCCCGCTGGGCTGGACCATCCCTGCTGCGCTGGGCGTGGTCAAAGCCGATCCGAAGCGCAAGGTTGTGGCGCTGTCGGGTGACTACGACTTCCAGTTCATGATCGAAGAACTGGCGGTAGGCGCCCAGTTCAACCTGCCGTACGTCCACGTACTGGTGAACAACGCCTACCTGGGCCTGATCCGCCAGGCGCAGCGTGGCTTCGACATGGATTACTGTGTACAACTGGCGTTCGAGAACATCAACTCGACCGACGCTGCCACCTACGGTGTCGACCACGTCGCTGTGGTCG  >  NC_002947/4888196‑4888487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑