Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	493,398	A→G	5.8%	H283R (CAC→CGC)	PP_0405 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	493,398	0	A	G	5.8%	201.6 / 2.7	69	H283R (CAC→CGC)	PP_0405	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (34/31);  new base G (2/2);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

CAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAGCTGGGTGAACTGATTGCC  >  NC_002947/493252‑493543                                                                                                                                                   |                                                                                                                                                   cAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCAcc                                                                                                                                                  >  2:83858/1‑149 (MQ=255)       cGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAgg                                                                                                                                            <  8:390491/149‑1 (MQ=255)        gCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGt                                                                                                                                           <  5:261485/149‑1 (MQ=255)        gCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCCGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGt                                                                                                                                           <  1:132386/149‑1 (MQ=255)           tttGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGAt                                                                                                                                        >  7:323696/1‑149 (MQ=255)              gTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCgg                                                                                                                                     >  2:328005/1‑149 (MQ=255)                      gCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTg                                                                                                                             >  7:356180/1‑149 (MQ=255)                        cGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGcc                                                                                                                           >  4:274953/1‑149 (MQ=255)                             gcgcGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCAt                                                                                                                      <  2:13409/149‑1 (MQ=255)                               gcgGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCt                                                                                                                    >  1:12723/1‑149 (MQ=255)                               gcgGGTCGAAGGTTGGCCGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCt                                                                                                                    >  7:73888/1‑149 (MQ=255)                                     cGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCt                                                                                                                    <  1:104114/143‑1 (MQ=255)                                     cGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCt                                                                                                                    >  2:104114/1‑143 (MQ=255)                                     cGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCAcc                                                                                                              >  3:173265/1‑149 (MQ=255)                                      gAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGAACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCCGAGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACcg                                                                                                             >  4:286740/1‑149 (MQ=255)                                       aaGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCGCCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACcgc                                                                                                            >  4:356852/1‑149 (MQ=255)                                              gCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGc                                                                                                      <  2:12723/148‑1 (MQ=255)                                                  gggtactactGGCAGCTGGCGCTGGCGTGTGGCTTCGCGGTGCACGCGGAGTTGGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGc                                                                                                  <  6:19197/144‑1 (MQ=255)                                                  ggCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCAt                                                                                                                                  >  5:192755/1‑116 (MQ=255)                                                  ggCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCAt                                                                                                                                  <  6:192755/116‑1 (MQ=255)                                                      actactGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCGCCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGt                                                                                             <  3:356852/149‑1 (MQ=255)                                                      actactGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGt                                                                                             <  8:378982/149‑1 (MQ=255)                                                        tactGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCGCCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTa                                                                                           >  4:51004/1‑149 (MQ=255)                                                        tactGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGc                                                                                                      <  4:83547/138‑1 (MQ=255)                                                        tactGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGc                                                                                                      >  3:83547/1‑138 (MQ=255)                                                         actGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTAc                                                                                          >  1:70051/1‑149 (MQ=255)                                                                  aggcgcaggcgGCTGGCATCCCGGTGCACGCCGTGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTa                                                                                           >  5:136104/1‑139 (MQ=255)                                                                  aggcgcaggcgGCTGGCATCCCGGTGCACGCCGTGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTa                                                                                           <  6:136104/139‑1 (MQ=255)                                                                           cggcTGGCATCACGGTGCACGCCGAGTTCGAACCCTTCCACCGCGCCACTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCCCATCTCCCACCGCGACGGCAAGCCGCGTTAACTGGG‑CAACGTGCCGCGt                                                                       >  2:347600/1‑149 (MQ=255)                                                                            ggcTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGtt                                                                      >  1:75709/1‑149 (MQ=255)                                                                                 gCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCGCCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTttcttc                                                                 <  3:51004/149‑1 (MQ=255)                                                                                 gCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTttcttc                                                                 >  3:153019/1‑149 (MQ=255)                                                                                 gCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTttcttc                                                                 >  2:404121/1‑149 (MQ=255)                                                                                  cATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCg                                                                >  5:139538/1‑149 (MQ=255)                                                                                   aTCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGc                                                               <  8:356180/149‑1 (MQ=255)                                                                                   aTCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGc                                                               <  1:404121/149‑1 (MQ=255)                                                                                     cccGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATATGCCACCGCGACGGCAAGCCGCCTTACCTGGG‑CGACGTGCCGCGTTTCTTCGcc                                                              >  6:19788/1‑148 (MQ=255)                                                                                            cACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATaaa                                                      >  7:129131/1‑149 (MQ=255)                                                                                             aCGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAAt                                                     <  4:153019/149‑1 (MQ=255)                                                                                               gCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCt                                                                                        <  2:94322/113‑1 (MQ=255)                                                                                                ccGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGaa                                                  <  6:179161/149‑1 (MQ=255)                                                                                                  gAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCAGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGt                                                >  6:122196/1‑149 (MQ=255)                                                                                                     ttCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGAt                                             <  8:73888/149‑1 (MQ=255)                                                                                                     ttCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGAt                                             >  1:74185/1‑149 (MQ=255)                                                                                                     ttCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGAt                                             <  2:127208/149‑1 (MQ=255)                                                                                                         aaGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGt                                         >  5:301297/1‑149 (MQ=255)                                                                                                            ccTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGAt                                             >  1:15120/1‑142 (MQ=255)                                                                                                            ccTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGAt                                             <  2:15120/142‑1 (MQ=255)                                                                                                            ccTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGGTTCTTCGCCTAGATAAATGAAGTGATCGGTCGc                                      >  3:222031/1‑149 (MQ=255)                                                                                                                      cgcCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGTGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAAt                            <  1:83858/149‑1 (MQ=255)                                                                                                                         cggTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGAACGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACCTGCCGCGTTTCTTCGCCTATATAAATGAAGTCATCGGTCGCCGGCGCGAATTgg                         >  8:164283/3‑149 (MQ=255)                                                                                                                          aGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGc                        >  4:99447/1‑149 (MQ=255)                                                                                                                              aCCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTg                          >  4:275308/1‑143 (MQ=255)                                                                                                                              aCCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTg                          <  3:275308/143‑1 (MQ=255)                                                                                                                              aCCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAg                    <  6:139538/149‑1 (MQ=255)                                                                                                                               ccTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGGCCTACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAg                    <  1:328005/149‑1 (MQ=255)                                                                                                                               ccTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGc                                                               >  1:106110/1‑105 (MQ=255)                                                                                                                               ccTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGc                                                               <  2:106110/105‑1 (MQ=255)                                                                                                                                cTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAGCt                  >  7:121392/1‑149 (MQ=255)                                                                                                                                   aTGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAGCTgg                <  8:129131/148‑1 (MQ=255)                                                                                                                                    tGGGCGTGCAGCGGCACCTGCAGGTGATCGGCAACTTTGGCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAGCTGGGt              <  7:105733/149‑1 (MQ=255)                                                                                                                                        cGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTTGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAGCTGGGTGAAc          <  2:70051/149‑1 (MQ=255)                                                                                                                                        cGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAGCTGGGTGAAc          <  8:323696/149‑1 (MQ=255)                                                                                                                                         gTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAGCAGGGTGAACt         >  2:79176/1‑149 (MQ=255)                                                                                                                                            cAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAGCTGGGTGAACTGa       <  6:301297/148‑1 (MQ=255)                                                                                                                                             aGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAGCTGGGTGAACTGAtt     >  2:370073/1‑149 (MQ=255)                                                                                                                                              gCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAGCTGGGTGAACTGATTg    <  3:274953/149‑1 (MQ=255)                                                                                                                                               cGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAGCTGGGTGAACTGATTGc   <  3:286740/149‑1 (MQ=255)                                                                                                                                                  cACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAGCTGGGTGAACTGATTGcc  >  7:15185/1‑147 (MQ=255)                                                                                                                                                   aCCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAGCTgg                <  7:216661/132‑1 (MQ=255)                                                                                                                                                   aCCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAGCTgg                >  8:216661/1‑132 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CAAGGACGCCTTTGTCAGCTGGCCGCAGGCGCGGGTCGAAGGTTGGCTGGGCGACTACTGGCAGCAGGCGCAGGCGGCTGGCATCCCGGTGCACGCCGAGTTCGAAGCCTTCCACCGCGCCAGTGACCTGATGGGCGTGCAGCGGCACCTGAAGGTGATCGGCATCTTTGCCCGCATCTGCCACCGCGACGGCAAGCCGCGTTACCTGGG‑CGACGTGCCGCGTTTCTTCGCCTATATAAATGAAGTGATCGGTCGCCGGCCCGAATTGGCGGAGCTGGGTGAACTGATTGCC  >  NC_002947/493252‑493543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑