Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,105,668	G→A	6.6%	P815L (CCG→CTG)	ydiJ ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,105,668	0	G	A	6.6%	227.8 / 3.0	75	P815L (CCG→CTG)	ydiJ	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (36/34);  new base A (3/2);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACACCAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGCC  >  NC_002947/5105522‑5105811                                                                                                                                                    |                                                                                                                                                acacCAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGgc                                                                                                                                               >  5:106892/1‑149 (MQ=255)   acCAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGgcgc                                                                                                                                             <  2:331736/149‑1 (MQ=255)     cAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGgcgc                                                                                                                                             >  6:116259/1‑147 (MQ=255)      aGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCATTCGGCCAGGTCCTTGAGCTGCGTGTCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAg                                                                                                                                          <  7:190468/149‑1 (MQ=255)      aGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAg                                                                                                                                          <  6:83133/149‑1 (MQ=255)            aTCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATAc                                                                                                                                    >  2:66589/1‑149 (MQ=255)             tCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATAcc                                                                                                                                   >  8:154629/1‑149 (MQ=255)               gCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATAccc                                                                                                                                  <  4:321935/148‑1 (MQ=255)                      ccAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATAccc                                                                                                                                  >  5:354884/1‑141 (MQ=255)                      ccAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATAccc                                                                                                                                  <  6:354884/141‑1 (MQ=255)                        aGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAg                                                                                                                        >  5:275049/1‑149 (MQ=255)                          ccccACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTGCGCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAgg                                                                                                                       <  1:66589/149‑1 (MQ=255)                             caccaGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGt                                                                                                                   >  3:169103/1‑149 (MQ=255)                              accaGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTg                                                                                                                  >  5:212350/1‑149 (MQ=255)                               ccaGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTgg                                                                                                                 <  6:191851/149‑1 (MQ=255)                               ccaGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTgg                                                                                                                 <  3:87693/149‑1 (MQ=255)                                     gCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCCGTACAGCGCCaacg                                                                                                                                        <  3:407704/120‑4 (MQ=255)                                     gCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCCGTACAGCGCCaacg                                                                                                                                        >  4:407704/1‑117 (MQ=255)                                         acCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGa                                                                                                       <  1:1693/149‑1 (MQ=255)                                             cAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATg                                                                                                     >  8:190395/1‑147 (MQ=255)                                             cAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGaa                                                                                                   <  7:306155/149‑1 (MQ=255)                                              aaTCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAg                                                                                                  >  5:295429/1‑149 (MQ=255)                                                   gCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGa                                                                                             >  6:173731/1‑149 (MQ=255)                                                           cTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTgg                                                                                                                 >  3:401136/1‑121 (MQ=255)                                                           cTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTgg                                                                                                                 <  4:401136/121‑1 (MQ=255)                                                           cTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAgcg                                                                                     <  6:106892/149‑1 (MQ=255)                                                                gCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGtt                                                                                <  4:77291/149‑1 (MQ=255)                                                                   gCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTc                                                                              >  3:346210/1‑148 (MQ=255)                                                                    cGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCg                                                                             >  2:318319/1‑148 (MQ=255)                                                                     gTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGaa                                                                           <  6:212350/149‑1 (MQ=255)                                                                     gTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGaa                                                                           <  6:295429/149‑1 (MQ=255)                                                                          gTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGc                                                                      >  3:369011/1‑149 (MQ=255)                                                                            tGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCgg                                                                    <  7:154629/149‑1 (MQ=255)                                                                             gCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGaa                                                                           <  8:162906/141‑1 (MQ=255)                                                                             gCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGaa                                                                           >  7:162906/1‑141 (MQ=255)                                                                             gCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCgg                                                                    <  5:173731/148‑1 (MQ=255)                                                                              cGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCggg                                                                   <  4:346210/148‑1 (MQ=255)                                                                                   cGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAgg                                                             >  3:212826/1‑149 (MQ=255)                                                                                     cTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGc                                                                                                >  4:344073/1‑112 (MQ=255)                                                                                     cTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCg                                                           <  7:190395/149‑1 (MQ=255)                                                                                     cTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCg                                                           >  5:408917/1‑149 (MQ=255)                                                                                        ccTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTcc                                                        >  7:95142/1‑149 (MQ=255)                                                                                        ccTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTcc                                                        >  5:302593/1‑149 (MQ=255)                                                                                          ttGGCACACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGc                                                            <  2:209649/143‑1 (MQ=255)                                                                                          ttGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGc                                                            >  1:209649/1‑143 (MQ=255)                                                                                          ttGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTg                                                      >  8:237801/1‑149 (MQ=255)                                                                                            ggCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTg                                                      <  4:212826/147‑1 (MQ=255)                                                                                             gCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCgg                                                                    >  8:77258/1‑132 (MQ=255)                                                                                             gCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCgg                                                                    <  7:77258/132‑1 (MQ=255)                                                                                                        aaGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGaa                                                                                                   >  6:293521/1‑90 (MQ=255)                                                                                                        aaGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGaa                                                                                                   <  5:293521/90‑1 (MQ=255)                                                                                                          ggAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCAcc                                                  <  7:361381/137‑1 (MQ=255)                                                                                                          ggAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCAcc                                                  >  8:361381/1‑137 (MQ=255)                                                                                                           gAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAgg                                                                                                 <  2:108010/89‑1 (MQ=255)                                                                                                           gAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAgg                                                                                                 >  1:108010/1‑89 (MQ=255)                                                                                                           gAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGt                                                                  <  3:149640/120‑1 (MQ=255)                                                                                                           gAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGt                                                                  >  4:149640/1‑120 (MQ=255)                                                                                                           gAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGaa                                                               <  4:129304/123‑1 (MQ=255)                                                                                                           gAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGaa                                                               >  3:129304/1‑123 (MQ=255)                                                                                                                ccTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTcg                                <  6:275049/149‑1 (MQ=255)                                                                                                                ccTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGATct                                >  7:343335/1‑148 (MQ=255)                                                                                                                       gTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTggg                         >  1:248947/1‑149 (MQ=255)                                                                                                                         gCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGc                                                     >  3:317176/1‑119 (MQ=255)                                                                                                                         gCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGc                                                     <  4:317176/119‑1 (MQ=255)                                                                                                                          cAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCg                      >  8:225156/1‑149 (MQ=255)                                                                                                                            aCGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCggg                    <  3:289369/149‑1 (MQ=255)                                                                                                                                      gTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTc                                                         <  3:128901/102‑1 (MQ=255)                                                                                                                                      gTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTc                                                         >  4:128901/1‑102 (MQ=255)                                                                                                                                        tG‑GCGCCGTACAGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTgg                          >  2:4977/1‑131 (MQ=255)                                                                                                                                        tG‑GCGCCGTACAGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTgg                          <  1:4977/131‑1 (MQ=255)                                                                                                                                        ctcgcgcCGTACAGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATCAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGAGAAGGCGTCCTGCACCAGCACGATGCTGCCCTCGCGCTGGGCCGGGGTCAGCTcgcg         >  4:74792/4‑149 (MQ=255)                                                                                                                                           gcgcTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTcgcg         >  7:317787/1‑146 (MQ=255)                                                                                                                                           gcgcTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTcgcg         <  8:317787/146‑1 (MQ=255)                                                                                                                                               tGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGcc  >  3:392638/1‑149 (MQ=255)                                                                                                                                                 tACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGcc  <  4:169103/147‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                ACACCAGCGTCATCGCCGGGTCCAGCCCCACCAGCGGCACACCGCAATCGGCCAGGGCCTTGAGCTGCGTGGCGTTGCGGATCGCTGCCTTGGCAAACGCGCCAAGGAAACCCTGTACGTGCAACGGCTTGCCGTTG‑GCGCTGTACGGCGCCAGGAATACCCGGTGGCCCAGGCGGTGGGCAAGGTCGATGAAGGCCGACAGCAGCGGCGTTTCGAAGTAGCGGGTGAAGGCGTCCTGCACCAGCACGATGCTGCGCTCGCGCTGGGCCGGGGTCAGCTCGCGCAGGGCC  >  NC_002947/5105522‑5105811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑