Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,162,630 G→T 9.8% intergenic (+153/+48) PP_4544 → / ← PP_4545 hypothetical protein/beta‑ketodecanoyl‑ACP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,162,6300GT 9.8% 119.0 / 4.5 41intergenic (+153/+48)PP_4544/PP_4545hypothetical protein/beta‑ketodecanoyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base G (19/18);  new base T (2/2);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

CTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTG  >  NC_002947/5162494‑5162776
                                                                                                                                        |                                                                                                                                                  
ctTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCggg                                                                                                                                          <  4:108715/147‑1 (MQ=255)
              ggAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCaa                                                                                                                                       <  2:292541/136‑1 (MQ=255)
              ggAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTCTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCaa                                                                                                                                       >  1:292541/1‑136 (MQ=255)
                aaaCGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGc                                                                                                                                     <  8:342987/136‑1 (MQ=255)
                aaaCGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGc                                                                                                                                     >  7:342987/1‑136 (MQ=255)
                                                      tCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAAt                                                                                   <  1:110228/148‑1 (MQ=255)
                                                                 tGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAAt                                                                       <  2:239549/149‑1 (MQ=255)
                                                                                    gCTTGCCCGCTCCCACAGTATCACCACATACTTTGATAACTGTGCTGTACCTGTGGGAGCGGGAAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAAc                                                    <  7:142565/149‑1 (MQ=255)
                                                                                      ttGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTTTGGGAGCGGGCAAGCCCGCGAAGAGGCCTTGACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATc                                                                      >  8:75763/1‑129 (MQ=255)
                                                                                      ttGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTTTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTGACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATc                                                                      <  7:75763/129‑1 (MQ=255)
                                                                                      ttGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTTTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTGACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATc                                                                      <  7:81447/129‑1 (MQ=255)
                                                                                      ttGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTTTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTGACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATc                                                                      >  8:81447/1‑129 (MQ=255)
                                                                                      ttGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGa                                                  >  2:385421/1‑149 (MQ=255)
                                                                                      tggCCCGCTCCCACCGTATCACCACAGACCTTGAGAACTGGGCTGTACCTGGGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGa                                                  <  2:126815/147‑1 (MQ=255)
                                                                                         cccGCTCCCACAGTATCACCACAGACCTTCAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCt                                               >  5:20911/1‑149 (MQ=255)
                                                                                                 cacaGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGc                                       >  1:179695/1‑149 (MQ=255)
                                                                                                      tATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAAc                                  >  2:185813/1‑149 (MQ=255)
                                                                                                         caccacAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCAGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAg                               >  6:36767/1‑149 (MQ=255)
                                                                                                          accacAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGc                              >  1:141947/1‑149 (MQ=255)
                                                                                                          accacAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCACCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACCAGCTCAACACGCACAACTAGc                              >  8:261670/1‑149 (MQ=255)
                                                                                                            cacaGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCaa                                            >  7:98024/1‑133 (MQ=255)
                                                                                                            cacaGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCaa                                            <  8:98024/133‑1 (MQ=255)
                                                                                                            cacaGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCt                            >  5:182658/1‑149 (MQ=255)
                                                                                                              caGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCttt                          <  1:385421/149‑1 (MQ=255)
                                                                                                               aGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCacac                                                                              >  6:253008/1‑96 (MQ=255)
                                                                                                               aGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCacac                                                                              <  5:253008/96‑1 (MQ=255)
                                                                                                               aGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTg                         <  1:185813/149‑1 (MQ=255)
                                                                                                                 aCCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGc                       <  6:20911/149‑1 (MQ=255)
                                                                                                                    ttGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGgc                                                                                                               >  6:248896/1‑58 (MQ=255)
                                                                                                                    ttGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGgc                                                                                                               <  5:248896/58‑1 (MQ=255)
                                                                                                                      gagaACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAg                    <  2:179695/147‑1 (MQ=255)
                                                                                                                      gagaACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGt                  >  5:292786/1‑149 (MQ=255)
                                                                                                                      gagaACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATATAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAg                    <  6:182658/147‑1 (MQ=255)
                                                                                                                        gaACTGTGCTGTACCTGTTGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCa                <  5:36767/149‑1 (MQ=255)
                                                                                                                            tgtgCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCGCCAAACGAGCTCAACACGCCCAACGCGCCTTTGGCCAGGTCATCCt            >  8:383820/1‑149 (MQ=255)
                                                                                                                                cTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGt         >  6:284444/1‑148 (MQ=255)
                                                                                                                                 tGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATAccc                                                              <  3:222301/94‑1 (MQ=255)
                                                                                                                                 tGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATAccc                                                              >  4:222301/1‑94 (MQ=255)
                                                                                                                                    aCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCaa                                   >  7:9044/1‑118 (MQ=255)
                                                                                                                                    aCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCaa                                   <  8:9044/118‑1 (MQ=255)
                                                                                                                                      cTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTg  >  1:351957/1‑149 (MQ=255)
                                                                                                                                       tgtgGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCa                     >  2:315929/1‑129 (MQ=255)
                                                                                                                                       tgtgGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCa                     <  1:315929/129‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                  
CTTCACCTGAAGCGGGAAACGCCGACCCCATCCGGCGTTTCCCGCTTCTTTTTGTCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTG  >  NC_002947/5162494‑5162776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑