Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,162,661 A→G 8.5% intergenic (+184/+17) PP_4544 → / ← PP_4545 hypothetical protein/beta‑ketodecanoyl‑ACP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,162,6610AG 8.5% 127.1 / 3.5 47intergenic (+184/+17)PP_4544/PP_4545hypothetical protein/beta‑ketodecanoyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base A (24/19);  new base G (2/2);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCC  >  NC_002947/5162548‑5162794
                                                                                                                 |                                                                                                                                     
tCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAAt                                                                                                     <  1:110228/148‑1 (MQ=255)
           tGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAAt                                                                                         <  2:239549/149‑1 (MQ=255)
                              gCTTGCCCGCTCCCACAGTATCACCACATACTTTGATAACTGTGCTGTACCTGTGGGAGCGGGAAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAAc                                                                      <  7:142565/149‑1 (MQ=255)
                                ttGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTTTGGGAGCGGGCAAGCCCGCGAAGAGGCCTTGACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATc                                                                                        >  8:75763/1‑129 (MQ=255)
                                ttGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTTTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTGACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATc                                                                                        >  8:81447/1‑129 (MQ=255)
                                ttGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTTTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTGACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATc                                                                                        <  7:75763/129‑1 (MQ=255)
                                ttGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTTTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTGACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATc                                                                                        <  7:81447/129‑1 (MQ=255)
                                ttGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGa                                                                    >  2:385421/1‑149 (MQ=255)
                                tggCCCGCTCCCACCGTATCACCACAGACCTTGAGAACTGGGCTGTACCTGGGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGa                                                                    <  2:126815/147‑1 (MQ=255)
                                   cccGCTCCCACAGTATCACCACAGACCTTCAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCt                                                                 >  5:20911/1‑149 (MQ=255)
                                           cacaGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGc                                                         >  1:179695/1‑149 (MQ=255)
                                                tATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAAc                                                    >  2:185813/1‑149 (MQ=255)
                                                   caccacAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCAGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAg                                                 >  6:36767/1‑149 (MQ=255)
                                                    accacAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGc                                                >  1:141947/1‑149 (MQ=255)
                                                    accacAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCACCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACCAGCTCAACACGCACAACTAGc                                                >  8:261670/1‑149 (MQ=255)
                                                      cacaGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCaa                                                              >  7:98024/1‑133 (MQ=255)
                                                      cacaGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCaa                                                              <  8:98024/133‑1 (MQ=255)
                                                      cacaGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCt                                              >  5:182658/1‑149 (MQ=255)
                                                        caGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCttt                                            <  1:385421/149‑1 (MQ=255)
                                                         aGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCacac                                                                                                >  6:253008/1‑96 (MQ=255)
                                                         aGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCacac                                                                                                <  5:253008/96‑1 (MQ=255)
                                                         aGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTg                                           <  1:185813/149‑1 (MQ=255)
                                                           aCCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGc                                         <  6:20911/149‑1 (MQ=255)
                                                              ttGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGgc                                                                                                                                 >  6:248896/1‑58 (MQ=255)
                                                              ttGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGgc                                                                                                                                 <  5:248896/58‑1 (MQ=255)
                                                                gagaACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAg                                      <  2:179695/147‑1 (MQ=255)
                                                                gagaACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGt                                    >  5:292786/1‑149 (MQ=255)
                                                                gagaACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATATAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAg                                      <  6:182658/147‑1 (MQ=255)
                                                                  gaACTGTGCTGTACCTGTTGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCa                                  <  5:36767/149‑1 (MQ=255)
                                                                      tgtgCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCGCCAAACGAGCTCAACACGCCCAACGCGCCTTTGGCCAGGTCATCCt                              >  8:383820/1‑149 (MQ=255)
                                                                          cTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGt                           >  6:284444/1‑148 (MQ=255)
                                                                           tGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATAccc                                                                                >  4:222301/1‑94 (MQ=255)
                                                                           tGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATAccc                                                                                <  3:222301/94‑1 (MQ=255)
                                                                              aCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCaa                                                     <  8:9044/118‑1 (MQ=255)
                                                                              aCCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCaa                                                     >  7:9044/1‑118 (MQ=255)
                                                                                cTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTg                    >  1:351957/1‑149 (MQ=255)
                                                                                 tgtgGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCa                                       <  1:315929/129‑1 (MQ=255)
                                                                                 tgtgGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCa                                       >  2:315929/1‑129 (MQ=255)
                                                                                   tgGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGa                  <  2:141947/148‑1 (MQ=255)
                                                                                          gggCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGTCAAt           >  4:303014/1‑148 (MQ=255)
                                                                                           ggCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTg                             >  8:246770/1‑129 (MQ=255)
                                                                                           ggCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTg                             <  7:246770/129‑1 (MQ=255)
                                                                                           ggCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGAACCAAACCAGCTCAACACGCACAACGATCCTTAGGCCACGTAAACCTCGTACAGGTGGAACGAAATCa         >  7:34208/1‑149 (MQ=255)
                                                                                                  ccGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCATACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGCTCATCCTGGTACAGGTGGAAGGCAATCCCCGAccc  >  1:308478/1‑149 (MQ=255)
                                                                                                  ccGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGc                                                                  <  6:401378/85‑1 (MQ=255)
                                                                                                  ccGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGc                                                                  >  5:401378/1‑85 (MQ=255)
                                                                                                  ccGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGAccc  >  6:153695/1‑149 (MQ=255)
                                                                                                                 |                                                                                                                                     
TCTGTGTGAAATGCGCCGGCCCCTTCGCGGGCTTGCCCGCTCCCACAGTATCACCACAGACCTTGAGAACTGTGCTGTACCTGTGGGAGCGGGCAAGCCCGCGAAGAGGCCGTAACAGGCGCCTGATGAATCAGCGCTTGCGCAGAATCACACTACCAATCGAATACCCCGCACCAAACGAGCTCAACACGCCCAACGAGCCTTTGGCCAGGTCATCCTGGTACAGGTGGAAGGCAATCACCGACCC  >  NC_002947/5162548‑5162794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑