Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,163,778 C→T 7.1% G25S (GGC→AGC)  PP_4545 ← beta‑ketodecanoyl‑ACP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,163,7780CT 7.1% 163.5 / 2.0 56G25S (GGC→AGC) PP_4545beta‑ketodecanoyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base C (27/25);  new base T (2/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-01

ACGCCGCGTCGGAGAGCGGCGCCGCCTCGACTTCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGG  >  NC_002947/5163631‑5163924
                                                                                                                                                   |                                                                                                                                                  
aCGCCGCGTCGGAGAGCGGCGCCGCCTCGACTTCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCg                                                                                                                                                   >  3:228016/1‑149 (MQ=255)
aCGCCGCGTCGGAGAGCGGCGCCGCCTCGACTTCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCg                                                                                                                                                   >  3:194269/1‑149 (MQ=255)
 cgccgcGTCGGAGAGCGGCGCCGCCTCGACTTCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCgg                                                                                                                                                  <  8:164185/149‑2 (MQ=255)
   ccgcGTCGGAGAGCGGCGCCGCCTCGACTTCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGACACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCt                                                                                                                                                 >  8:109900/1‑148 (MQ=255)
    cgcgTCGGAGAGCGGCGCCGCCTCGACTTCACCGCGCTCGATGGCGGCAGCGTTGTCCTGATTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGa                                                                                                                                               >  1:116630/1‑149 (MQ=255)
     gcgTCGGAGAGCGGCGCCGCCTCGACTTCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGa                                                                                                                                               >  7:106142/1‑148 (MQ=255)
                    gccgccTCGACTTCACCGCGCTCGATGGCGGCAGCGTTGTGCTGGTTGAACTGCTGCGACCAGGCATTGAAGTAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCAc                                                                                                                               <  6:258731/149‑1 (MQ=255)
                      cgccTCGACTTCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACgg                                                                                                                             <  4:181904/149‑1 (MQ=255)
                        ccTCGACTTCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGATCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTc                                                                                                                           >  3:66315/1‑149 (MQ=255)
                             aCTTCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTcc                                                                                                                      <  4:228016/149‑1 (MQ=255)
                               ttCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCac                                                                                                                                           >  2:17358/1‑126 (MQ=255)
                               ttCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCac                                                                                                                                           <  1:17358/126‑1 (MQ=255)
                                  aCCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTgg                                                                                                                 >  8:152306/1‑149 (MQ=255)
                                       gcTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACTGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCa                                                                                                              >  1:244767/1‑147 (MQ=255)
                                                  gcAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCca                                                                                                 <  8:333214/149‑1 (MQ=255)
                                                   cAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCcac                                                                                                <  8:106142/149‑1 (MQ=255)
                                                        ttGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGgc                                                                                           <  4:76812/149‑1 (MQ=255)
                                                             cTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACTGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCgctg                                                                                       <  2:244767/148‑1 (MQ=255)
                                                                gTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCgctggctg                                                                                   <  8:151409/149‑1 (MQ=255)
                                                                gTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCgctggct                                                                                    <  6:140434/148‑1 (MQ=255)
                                                                 ttGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCgctggctg                                                                                   >  5:115266/1‑148 (MQ=255)
                                                                     aCTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGaaa                                                                               <  6:115266/148‑1 (MQ=255)
                                                                       tgctgcGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCTGCTGATCATGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGa                                                                                 <  5:372170/144‑1 (MQ=255)
                                                                       tgctgcGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCTGCTGATCATGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGa                                                                                 >  6:365793/1‑144 (MQ=255)
                                                                       tgctgcGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCTGCTGATCATGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGa                                                                                 <  5:365793/144‑1 (MQ=255)
                                                                       tgctgcGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCTGCTGATCATGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGa                                                                                 >  6:372170/1‑144 (MQ=255)
                                                                       tgctgcGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACAt                                                                            >  7:315718/1‑149 (MQ=255)
                                                                         ctgcGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGa                                                                          <  2:266122/149‑1 (MQ=255)
                                                                          tgcGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGAc                                                                         <  8:315718/149‑1 (MQ=255)
                                                                                  ggCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAAt                                                                 <  3:299011/149‑1 (MQ=255)
                                                                                  ggCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAAt                                                                 <  7:109900/149‑1 (MQ=255)
                                                                                   tcATTGAAGTAAGCCAGCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTTATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATg                                                                <  2:99486/148‑1 (MQ=255)
                                                                                     aTTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATgg                                                               >  3:74456/1‑148 (MQ=255)
                                                                                     aTTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATgg                                                               >  3:189900/1‑148 (MQ=255)
                                                                                      ctGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGc                                                              <  7:341164/147‑1 (MQ=255)
                                                                                       tGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCAc                                                            >  4:166566/1‑149 (MQ=255)
                                                                                        gaaggaagCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACt                                                           >  3:35371/1‑149 (MQ=255)
                                                                                         aaggaagCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGAGAGGTTAATGGCACtt                                                          >  6:333660/1‑149 (MQ=255)
                                                                                         aaggaagCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGc                                                              >  8:341164/1‑145 (MQ=255)
                                                                                                ccaccaGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGc                                                    >  1:16060/1‑148 (MQ=255)
                                                                                                 caccaGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGACGGTGCCGCTGATCACGACGTTATGCACGGACGTTCCTCTGTTCAAAGGACATCGACACAGGGCGCTGGCTGGAAACATGACAGGTTatt                                                                 >  4:384330/1‑132 (MQ=255)
                                                                                                  accaGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTAAAGTGCCat                                                 <  4:194269/149‑2 (MQ=255)
                                                                                                                  aaaTGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGtt                                 >  3:319462/1‑149 (MQ=255)
                                                                                                                   aaTGCTCTGGGCCGGGGTGTACA‑GCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTa                               >  7:184888/1‑149 (MQ=255)
                                                                                                                     tGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTAc                              >  8:272294/1‑149 (MQ=255)
                                                                                                                        tctGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGt                                                                     >  7:255692/1‑107 (MQ=255)
                                                                                                                        tctGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGt                                                                     <  8:255692/107‑1 (MQ=255)
                                                                                                                             gCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACa                                                                        <  2:71607/99‑1 (MQ=255)
                                                                                                                             gCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACa                                                                        >  1:71607/1‑99 (MQ=255)
                                                                                                                                    tgtACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCgcgccgttc               <  4:74456/149‑4 (MQ=255)
                                                                                                                                    tgtACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCgcgccgttc               <  4:189900/149‑4 (MQ=255)
                                                                                                                                        cAGGCCGGTGCCGCTGATCACGACTTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTAt           >  8:378655/1‑149 (MQ=255)
                                                                                                                                         aGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACGGGTTAATGGCAGTTTAGt                                                      >  7:217605/1‑105 (MQ=255)
                                                                                                                                         aGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGt                                                      <  8:217605/105‑1 (MQ=255)
                                                                                                                                         aGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATc          <  3:166566/149‑1 (MQ=255)
                                                                                                                                                 gCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAgg  >  2:211843/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACGCCGCGTCGGAGAGCGGCGCCGCCTCGACTTCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGG  >  NC_002947/5163631‑5163924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑