Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,163,787 C→T 7.4% V22M (GTG→ATG)  PP_4545 ← beta‑ketodecanoyl‑ACP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,163,7870CT 7.4% 158.7 / 2.1 54V22M (GTG→ATG) PP_4545beta‑ketodecanoyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base C (25/25);  new base T (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01

GCCGCCTCGACTTCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAA  >  NC_002947/5163651‑5163932
                                                                                                                                        |                                                                                                                                                 
gccgccTCGACTTCACCGCGCTCGATGGCGGCAGCGTTGTGCTGGTTGAACTGCTGCGACCAGGCATTGAAGTAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCAc                                                                                                                                       <  6:258731/149‑1 (MQ=255)
  cgccTCGACTTCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACgg                                                                                                                                     <  4:181904/149‑1 (MQ=255)
    ccTCGACTTCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGATCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTc                                                                                                                                   >  3:66315/1‑149 (MQ=255)
         aCTTCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTcc                                                                                                                              <  4:228016/149‑1 (MQ=255)
           ttCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCac                                                                                                                                                   <  1:17358/126‑1 (MQ=255)
           ttCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCac                                                                                                                                                   >  2:17358/1‑126 (MQ=255)
              aCCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTgg                                                                                                                         >  8:152306/1‑149 (MQ=255)
                   gcTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACTGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCa                                                                                                                      >  1:244767/1‑147 (MQ=255)
                              gcAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCca                                                                                                         <  8:333214/149‑1 (MQ=255)
                               cAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCcac                                                                                                        <  8:106142/149‑1 (MQ=255)
                                    ttGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGgc                                                                                                   <  4:76812/149‑1 (MQ=255)
                                         cTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACTGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCgctg                                                                                               <  2:244767/148‑1 (MQ=255)
                                            gTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCgctggctg                                                                                           <  8:151409/149‑1 (MQ=255)
                                            gTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCgctggct                                                                                            <  6:140434/148‑1 (MQ=255)
                                             ttGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCgctggctg                                                                                           >  5:115266/1‑148 (MQ=255)
                                                 aCTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGaaa                                                                                       <  6:115266/148‑1 (MQ=255)
                                                   tgctgcGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCTGCTGATCATGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGa                                                                                         <  5:372170/144‑1 (MQ=255)
                                                   tgctgcGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCTGCTGATCATGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGa                                                                                         <  5:365793/144‑1 (MQ=255)
                                                   tgctgcGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCTGCTGATCATGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGa                                                                                         >  6:365793/1‑144 (MQ=255)
                                                   tgctgcGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCTGCTGATCATGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGa                                                                                         >  6:372170/1‑144 (MQ=255)
                                                   tgctgcGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACAt                                                                                    >  7:315718/1‑149 (MQ=255)
                                                     ctgcGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGa                                                                                  <  2:266122/149‑1 (MQ=255)
                                                      tgcGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGAc                                                                                 <  8:315718/149‑1 (MQ=255)
                                                              ggCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAAt                                                                         <  7:109900/149‑1 (MQ=255)
                                                              ggCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAAt                                                                         <  3:299011/149‑1 (MQ=255)
                                                               tcATTGAAGTAAGCCAGCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTTATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATg                                                                        <  2:99486/148‑1 (MQ=255)
                                                                 aTTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATgg                                                                       >  3:74456/1‑148 (MQ=255)
                                                                 aTTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATgg                                                                       >  3:189900/1‑148 (MQ=255)
                                                                  ctGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGc                                                                      <  7:341164/147‑1 (MQ=255)
                                                                   tGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCAc                                                                    >  4:166566/1‑149 (MQ=255)
                                                                    gaaggaagCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACt                                                                   >  3:35371/1‑149 (MQ=255)
                                                                     aaggaagCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGAGAGGTTAATGGCACtt                                                                  >  6:333660/1‑149 (MQ=255)
                                                                     aaggaagCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGc                                                                      >  8:341164/1‑145 (MQ=255)
                                                                            ccaccaGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGc                                                            >  1:16060/1‑148 (MQ=255)
                                                                             caccaGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGACGGTGCCGCTGATCACGACGTTATGCACGGACGTTCCTCTGTTCAAAGGACATCGACACAGGGCGCTGGCTGGAAACATGACAGGTTatt                                                                         >  4:384330/1‑132 (MQ=255)
                                                                              accaGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTAAAGTGCCat                                                         <  4:194269/149‑2 (MQ=255)
                                                                                              aaaTGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGtt                                         >  3:319462/1‑149 (MQ=255)
                                                                                               aaTGCTCTGGGCCGGGGTGTACA‑GCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTa                                       >  7:184888/1‑149 (MQ=255)
                                                                                                 tGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTAc                                      >  8:272294/1‑149 (MQ=255)
                                                                                                    tctGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGt                                                                             <  8:255692/107‑1 (MQ=255)
                                                                                                    tctGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGt                                                                             >  7:255692/1‑107 (MQ=255)
                                                                                                         gCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACa                                                                                <  2:71607/99‑1 (MQ=255)
                                                                                                         gCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACa                                                                                >  1:71607/1‑99 (MQ=255)
                                                                                                                tgtACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCgcgccgttc                       <  4:74456/149‑4 (MQ=255)
                                                                                                                tgtACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCgcgccgttc                       <  4:189900/149‑4 (MQ=255)
                                                                                                                    cAGGCCGGTGCCGCTGATCACGACTTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTAt                   >  8:378655/1‑149 (MQ=255)
                                                                                                                     aGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACGGGTTAATGGCAGTTTAGt                                                              >  7:217605/1‑105 (MQ=255)
                                                                                                                     aGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGt                                                              <  8:217605/105‑1 (MQ=255)
                                                                                                                     aGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATc                  <  3:166566/149‑1 (MQ=255)
                                                                                                                             gCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAgg          >  2:211843/1‑149 (MQ=255)
                                                                                                                                gCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGcc       >  5:319307/1‑149 (MQ=255)
                                                                                                                                gCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGcc       >  4:104556/1‑149 (MQ=255)
                                                                                                                                 cTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCg      <  2:16060/149‑1 (MQ=255)
                                                                                                                                  tGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCg      >  6:13875/1‑148 (MQ=255)
                                                                                                                                      cACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCaaa  <  3:114197/148‑1 (MQ=255)
                                                                                                                                      cACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCa    >  4:114197/1‑146 (MQ=255)
                                                                                                                                        |                                                                                                                                                 
GCCGCCTCGACTTCACCGCGCTCGATGGCGGCAGCGTTGTCCTGGTTGAACTGCTGCGACCAGGCATTGAAGGAAGCCACCAGCTCTTCGTTGGAAATGCTCTGGGCCGGGGTGTACAGGCCGGTGCCGCTGATCACGACGTTATGCACGGTCGTTCCTCTGGTCAAAGGCCATCGCCACAGGGCGCTGGCTGGAAACATGACAGGTTAATGGCACTTTAGTGCCAATTCAGGGGCATACGTTTACCCCCGCGCGGCGGGCTTATCCGCCAAGGGCCGCAAA  >  NC_002947/5163651‑5163932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑